Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,647,949	C→T	intergenic (‑161/‑114)	dinQ ← / → agrA	membrane toxin DinQ/small RNA AgrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,647,949	0	C	T	100.0%	57.2 / NA	19	intergenic (‑161/‑114)	dinQ/agrA	membrane toxin DinQ/small RNA AgrA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/13);  total (6/13)

GCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCATCCGCTTACTCATCCACTGCCTGTCACGGCGCATGTCTCATTGTTAGATAAGAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGA  >  NC_000913/3647729‑3648188                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                gCGGATCAGTTCCAGCATCTCAATTAACGCCCCTAGATCGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCATCCGCTTACTCATCCACTGCCTGTCACGGCGCATGTCTCATTGTTAGATAAGAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTGTGAAAGCGTTTATGTGCATTGCCAGAGTGAAAATTCCTCAGAGCt                                                                                                                                                                                                                   <  1:204515/251‑1 (MQ=255)        aGTTCAAGCAGCGCAATTAACGCCCCTAGAACGCTGCTTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCATCCGCTTACTCATCCACTGCCTGTCACGGCGCATGTCTCATTGTTAGATAAGAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAACAGTTTATGTGCATTGCCAGAGTTAAAATGCCTCAGAGCTAACTgg                                                                                                                                                                                                             >  2:51190/1‑250 (MQ=255)          ttCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCATCCGCTTACTCATCCACTGCCTGTAACGGCGCATGTCTCATTGTTAGATAAGAACTCTCTCACTACGGCCAGAGCATAAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGTGCATTGCCAGAGTGACAATGCCTAAGAGCTAACTGGAt                                                                                                                                                                                                           >  1:27140/1‑250 (MQ=255)                cagcGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATTCATCCGCTTACTCATCCACTGCCTGTCACGGCGCATGTCTCATTGTTAGATAAGAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGTGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATa                                                                                                                                                                                                    <  1:247911/251‑1 (MQ=255)                           aCGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCATCCGCTTACTCATCCACTGCCTGTCACGGCGCATGTCTCATTGTTAGATAAGAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTTAAAGCGTTTATGTGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGcc                                                                                                                                                                                         <  2:37315/251‑2 (MQ=255)                                     aactatgatgGCTTTATCAATCACCCGTTTGCTGCATGCGATGGAGGGAGAATGCATCCGCTTACTCATCCACTGCCTGTCACGGCGCATGTCTCATTGTTAGATAATAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGTGCATTGCCAGAGTGAAAATGACACAGAGCTAACGGGATAATCATACAGTACATGCAGGTTATaaaa                                                                                                                                                                               <  2:12716/247‑1 (MQ=255)                                              tGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCATCCGCTTACTCATCCACTGCCTGTCACGGCGCATGTCTCATTGTTAGATAAGAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGTGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACg                                                                                                                                                                       <  2:90390/250‑1 (MQ=255)                                                    atcaatcaCCCGTTTTCTCCATGCGATGGAGTGAGAATGCATCCGCTTACTCATCCACTGCCTGTCACGGCGCATGTCTCATTGTTAGATAATAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGTCTTTGAAAGCGTTTATGTGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTg                                                                                                                                                                 <  1:183101/250‑1 (MQ=255)                                                        atcaCCCGTTTTCTCCATGCGATGGAGTGAGAATGCATCCGCTTACTCATCCACTGCCTGTCACGGCGCATGTCTCATTGTTAGATAAGAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGTGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGCTAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCact                                                                                                                                                             >  2:134817/1‑248 (MQ=255)                                                                      ccATGCGATGGAGTGAGAATGCATCCGCTTACTCATCCACTGCCTGTCACGGCGCATGTCTCATTGTTAGATAAGAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCTTACTTCTGACCAGGACTTTGAAAGCGTTTATGTGCATTGCCAGAGTGAAAATGTCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGt                                                                                                                                               <  1:176970/250‑1 (MQ=255)                                                                      ccATGCGATGGAGTGAGAATGCATCCGCTTACTCATCCACTGCCTGTCACGGCGCATGTCTCATTGTTAGATAAGAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTGTATGTGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATg                                                                                                                                                 <  1:57533/248‑1 (MQ=255)                                                                                                       cATCCACTGCCTGTCACGGCGCATGTCTCATTGTTAGATAAGAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGTGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCa                                                                                                             <  1:41760/251‑1 (MQ=255)                                                                                                                           gcATGTCTCATTGTTAGATAAGAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGTGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAAc                                                                                         <  1:83965/251‑1 (MQ=255)                                                                                                                            cATGTCTCATTGTTAGATAAGAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGTGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTAtct                                                                                                               <  1:83156/228‑1 (MQ=255)                                                                                                                            cATGTCTCATTGTTAGATAAGAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGTGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTAtct                                                                                                               >  2:83156/1‑228 (MQ=255)                                                                                                                                                                gCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGTGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCAGACAGTACATGCAGGTTATAAAACCAGCACGTCCGTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGt                                                    <  1:112543/251‑1 (MQ=255)                                                                                                                                                                                                        aGGACTTTGAAAGCGTTTATGTGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGAt            >  1:206196/1‑251 (MQ=255)                                                                                                                                                                                                               tGAAAGCGTTTATGTGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCa      <  1:8415/250‑1 (MQ=255)                                                                                                                                                                                                                   aGCGTTTATGTGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGa  >  2:118901/1‑250 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                GCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAATGCATCCGCTTACTCATCCACTGCCTGTCACGGCGCATGTCTCATTGTTAGATAAGAACTCTCTCACTCCGGCCAGAGCATCAGTTAACGGCACCACCCGTACTTCTGACCAGGACTTTGAAAGCGTTTATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGA  >  NC_000913/3647729‑3648188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑