Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,511,466	C→T	V293V (GTG→GTA)	yhfZ ←	putative DNA‑binding transcriptional regulator YhfZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,511,466	0	C	T	100.0%	120.8 / NA	40	V293V (GTG→GTA)	yhfZ	putative DNA‑binding transcriptional regulator YhfZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/21);  total (19/21)

CTCGTTATCCAGCGGCTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAA  >  NC_000913/3511219‑3511706                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 cccGTTATCCAGCGTCTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCcacttact                                                                                                                                                                                                                                               <  2:226726/249‑1 (MQ=255)                 tctATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATg                                                                                                                                                                                                                                 <  2:222271/249‑1 (MQ=255)                 tctATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATg                                                                                                                                                                                                                                 >  1:222271/1‑249 (MQ=255)                               ccGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCgtgt                                                                                                                                                                                                                 <  2:48442/250‑1 (MQ=255)                               acGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCgtgt                                                                                                                                                                                                                 <  1:66080/251‑1 (MQ=255)                                   cGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTAt                                                                                                                                                                                                             >  1:254070/1‑251 (MQ=255)                                       tGCGCCTCAGTGCCCTCCCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCATTACGGTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGACTCTGTTTTCCATATCAGATGCCTTGCGTGTAATAACTCTGTTCCGGTTCCCCACGTACTACCCGTTGTGGATGGGCGATCAGGGc                                                                                                                                                                                                                     <  2:249396/239‑1 (MQ=255)                                       tGCGCATAAGTGAACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCAATGAGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGAAGATGTCATTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCCGGGc                                                                                                                                                                                                                     >  1:249396/1‑239 (MQ=255)                                               aGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGCCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATCACGCCTGCCTCGCAAAGCAGGATGAGTCTGGTTTCCATATCAGCTGCCTTTCGTTTAATAACTCCGTTCCTGTTCACCACTTACTACCCGTTGTTGATGGGCGCGCAGGGCGTGTTTATCTACAACGGCAc                                                                                                                                                                                                 >  1:17906/1‑251 (MQ=255)                                                               gtgtCATCGCCATCGGTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGAAGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCAt                                                                                                                                                                                 >  2:111748/1‑251 (MQ=255)                                                                    aTCGCCATCGTTCCTTGCTCCCTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGt                                                                                                                                                                            >  2:131522/1‑251 (MQ=255)                                                                    aTCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGt                                                                                                                                                                            <  1:113424/251‑1 (MQ=255)                                                                                         cTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAgg                                                                                                                                                        >  1:80905/1‑250 (MQ=255)                                                                                           gcgcACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGa                                                                                                                                                       <  2:100727/249‑1 (MQ=255)                                                                                              cACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTACTCATCGCCTCGCGTCAGGCCCACg                                                                                                                                                  >  1:248286/1‑251 (MQ=255)                                                                                                  ggCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTAGCAAAGCAGGTTGAGTCTGGTTTTCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCtt                                                                                                                                              <  2:88020/251‑1 (MQ=255)                                                                                                                 gTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCGCTTACGACCCGTTGTTGATGGGCGAGCAGGGCGTGGTTATCTACAACGGCACGCAGCAGTTGTTGCATGGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTaaaa                                                                                                                               <  1:249946/251‑1 (MQ=255)                                                                                                                              tGACGACCAGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGATAATCATCGACTCGCGTCCGGACCACGGCTTCGGTGGCCTGTACAAATCGCGGACCGTc                                                                                                                  >  2:207110/1‑251 (MQ=255)                                                                                                                               gacgacCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCt                                                                                                                 <  1:187548/251‑1 (MQ=255)                                                                                                                                acgacCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGGAATCATCGACTCGCGTCAGGACCACGGCTTCGGTTGCCTGTAAAAATCGCGGATCGTCtg                                                                                                                <  2:254070/251‑1 (MQ=255)                                                                                                                                  gacCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCtgtg                                                                                                              >  2:196631/1‑251 (MQ=255)                                                                                                                                            atGCCTTTGCAGATGTCCTTATCACTAACGCCTGCCTCGCAAAGCACGTTGAGTCTGGTTTCCATGTCAGTTGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTTTCTACAACGGCACGCAGCAGTTGTTGCATTTGGTAATCATCGCCTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGCTCGTCGGTGAgcggcctcc                                                                                                    >  2:29236/1‑247 (MQ=255)                                                                                                                                             tGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGATTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGc                                                                                                   >  2:39597/1‑251 (MQ=255)                                                                                                                                              gacgtggtgctcttccgatctaaTAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGcc                                                                                                  <  1:136301/230‑1 (MQ=255)                                                                                                                                                   tGCAGATGTCCTCATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCAGCGACTCGCGTCAGGTCCACGGCGGCGGTGGCCTGTAAAAATCGCGGATCGTCTGTTAGCCGCGTCGCCTCTa                                                                                              <  1:223730/250‑1 (MQ=255)                                                                                                                                                     cAGAAGTCCTTCTCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTCTCTACAACGGCACGCAGCAGTTGATGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCTTCGCCTCTAATc                                                                                           >  2:223319/1‑251 (MQ=255)                                                                                                                                                             cTTATCAATAACGCCTGCCTGGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGCGCGGCGTCGCCTCTAATCCCAGCATg                                                                                   >  2:197160/1‑251 (MQ=255)                                                                                                                                                                   aaTAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGcc                                                                                                  >  2:136301/1‑230 (MQ=255)                                                                                                                                                                               ctCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGcc                                                                 >  1:161628/1‑251 (MQ=255)                                                                                                                                                                                      aGCAGGCTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCCCTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTCATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGt                                                          >  2:116452/1‑251 (MQ=255)                                                                                                                                                                                        cAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTc                                                        <  2:79266/251‑1 (MQ=255)                                                                                                                                                                                              gAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGAt                                                   <  1:69600/250‑1 (MQ=255)                                                                                                                                                                                                                aGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCt                                <  1:131237/251‑1 (MQ=255)                                                                                                                                                                                                                          cGTTTAATAACTCGGTTCCGGTTCCCCACTTACTACCCGTTGTGGAGGGGCGAGCAGGGCGGGTTTAGCTACAACGGCACGCAGCAGTTGTTGCAGTGGGTAATCATCGCCGCGCGTCAGGTCCACGGCTTCGGTGGCCTGGAAAAATCGCGGATCGTCTGGGAGCGGCGGCGCCGCTAATCCCAGCATGGGCAGTTCGTTTTCCGCCACCACGTGCCAGAGCACCGCATCGACATCGCCTTTAACAATGc                      <  2:264284/251‑1 (MQ=255)                                                                                                                                                                                                                             ttAATAACTCGGTTCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGt                    <  1:97783/250‑1 (MQ=255)                                                                                                                                                                                                                             ttAATAACGCGTTTCCTGTTCCCCACTTCCTACCCGTTGTTGATGGGCCAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCACCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGt                    <  2:80905/250‑1 (MQ=255)                                                                                                                                                                                                                               aataaCTCGGTTCCTGTTCCCCCCTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTCCAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGCTGt                 >  2:131988/1‑251 (MQ=255)                                                                                                                                                                                                                                       ggTTCCTGTTCCCCACTTACTACCCGTTGGTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACtctc         <  1:116452/251‑1 (MQ=255)                                                                                                                                                                                                                                       ggTTCCTGTCCCCCCCTTACTCCCCGTTGTTGAGGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACtctc         <  2:156377/251‑1 (MQ=255)                                                                                                                                                                                                                                          tCCTGTTCCCCACTTACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAAAGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTg      >  2:113491/1‑251 (MQ=255)                                                                                                                                                                                                                                              gTTCCCCACTTACTTCCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATaa  <  2:209776/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 CTCGTTATCCAGCGGCTCTATTTCTTCACCACGGCGACTGCGCATCAGTGCACTCGCCATATGTGTCATCGCCATCGTTCCTTGCTCACTGCGCACCGGCAGATGGCACTCTGTTTCCAGTACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAA  >  NC_000913/3511219‑3511706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑