Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,500,129	G→A	G74S (GGC→AGC)	frlA →	fructoselysine/psicoselysine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,500,129	0	G	A	92.0%	60.6 / ‑3.7	25	G74S (GGC→AGC)	frlA	fructoselysine/psicoselysine transporter
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (9/14);  total (10/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

ACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATC‑CGGAAAATGGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGTTTCAGACGC  >  NC_000913/3499882‑3500372                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    aCGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTTGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTTGCGGGTTAAGTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCTCCGCTTATC‑CGGAAAATAgcg                                                                                                                                                                                                                                                  <  2:259610/251‑1 (MQ=255)     aTTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATC‑CGGAAAATAGCGCAg                                                                                                                                                                                                                                               <  1:166230/250‑1 (MQ=255)             aGAAAAAGGTATCGCTATGGGAAGCCAGGAACGCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAAGCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGGCTATGCGGAACTATCCACCGCTTATC‑CGGAAAATAGCGCAGATTATGttt                                                                                                                                                                                                                                      <  1:116748/251‑1 (MQ=255)                          gCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGGTAATTGTGATCCCTCCAATATGGGTCTTAGCGGGACTATCCACCGATTTTC‑CGGACAATAgcggcgcttttgattatccgaaaacatc                                                                                                                                                                                                                         >  1:53649/1‑226 (MQ=255)                             aTGGGAAGCCAGGCACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGGTTATTGTGTTCCCGCCAATGGGCGTCTATGCGGGAATATCCACCGCTTTTA‑CGGCAAAAAACGCAGATTATGtttttttggaaaaagtcgg                                                                                                                                                                                                                      >  2:194024/1‑235 (MQ=255)                                     ccAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATC‑CGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGAc                                                                                                                                                                                                              <  1:132502/251‑1 (MQ=255)                                                        catggATTTGGGGCCGTGCTTGCAAACGCCGTCGGGATAACCGTCTGCTCCGGTATTTTTGGATCTGTTGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGTGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATC‑CGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCgg                                                                                                                                                                                           <  2:250540/248‑1 (MQ=255)                                                                                                              atctttGTATCGGTGGGTGAAGTGGAAAAAGCAGCGGGTTCGCCGTGGCTTACGGTGCCCGCGTTTGTCCTTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATC‑CGGAAAATGGCTCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTGCTGGGCCAACTATGGGCCGTCATTGTCGATTATGCCGCTgg                                                                                                                                      <  2:169186/247‑1 (MQ=255)                                                                                                                     tATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGGTAATTGTGATCCCGCCAATTTGGGTCTATGCGGAACTATCCACCGCCTATC‑CGGGAAAAAGCGCCGATTATGTTTTTCTGAAAAATGCCGGGAGACGACCGCTGGCTTTTCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCCTCATTGTTGCTTATGGCGCTGGCGATTGTc                                                                                                                              >  1:205184/1‑251 (MQ=255)                                                                                                                                 aaGTGGCAAAAGCAGCGGGAACGCCGGGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATC‑CGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGTGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGc                                                                                                                  <  2:147182/251‑1 (MQ=255)                                                                                                                                   gTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCACGCACACGTGCGCCTCTGCGGAACTATCCACCGCTTATCAGGGAAAACGG‑GCAGATTTTGTTTATCTGAACAATACCGGAAGCCGACCGCTGGGTTTCCTCTCCCGCTGGGCCAGCTTCTGGGCCAACGAAGAGCCGTCCTTGTCGCTTATGGCGCTGGCGGATGTCAGCAACCTTGGCtt                                                                                                                >  1:126081/1‑251 (MQ=255)                                                                                                                                        aaaaGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTTTGATCCCGCCAATGTGCGTCTATGCGGGACTATCCACCGCTTTTC‑CGGACAATAGCGCAGGTTTTGTTTTTTTGAAAAATGCCGGAAACCGACCCCTGGGTTTCCTCTCCGGCTGGGCCAGGTTCTGGGCCAACCATGCGCCGTCATTGTCGGTTATGGCGCTGGGGATTGTCACCAATTTTTGCtttttt                                                                                                            >  1:127198/1‑249 (MQ=255)                                                                                                                                                              tttACGGTGCTCGCGTTTGTCATTGGCGGGTTAATGGTTATCCCGCAAATGTGCTTCTATGCGGATCGAACCACCGCTTATC‑CGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAATCCGACCTCTTGCTTTCCTCTCCCGCTGGGCCAGCTTCTTGGCCAACTATGCGTCGTCATTGTCGATGATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTAGCGATCCGTTTCTCg                                                                                      <  1:71376/249‑1 (MQ=255)                                                                                                                                                                           cgTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATC‑CGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATcgcc                                                                        <  1:137400/251‑1 (MQ=255)                                                                                                                                                                               tGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATC‑CGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCTGTAAATTTATCgccgcc                                                                     <  2:135454/250‑1 (MQ=255)                                                                                                                                                                               tGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATC‑CGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCgccgcc                                                                     <  2:205184/250‑1 (MQ=255)                                                                                                                                                                                      ggCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATC‑CGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGattaat                                                             <  1:69150/251‑1 (MQ=255)                                                                                                                                                                                             tAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCCCCGCTTATC‑CGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTGCCGCTCCGGCTGGGCCAGCTTCTGGTCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCCGATTAATTATCGc                                                       <  2:53649/250‑1 (MQ=255)                                                                                                                                                                                                                gtgCGTCTATGCGGACCTATCCACAGCTTATC‑CGGACAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTgc                                   >  2:33044/1‑251 (MQ=255)                                                                                                                                                                                                                   cGTCTATGCGGAACTATCCACCGCTTATC‑CGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACCCCTGCGCt                                >  2:150202/1‑251 (MQ=255)                                                                                                                                                                                                                      cTATGCGGAACTATCCACCGCTTATC‑CGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCg                             >  1:173039/1‑251 (MQ=255)                                                                                                                                                                                                                           cGGAACTATCCACCGCTTATC‑CGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGa                         >  1:232779/1‑250 (MQ=255)                                                                                                                                                                                                                                  aTCCACCGCTTATC‑CGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACTCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGggggcg                 <  1:16534/251‑1 (MQ=255)                                                                                                                                                                                                                                               c‑cGGAAAATAGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGTTTCAGAc    <  1:14583/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cGGAAAATAGCGCAGATTATGTT‑ATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGAGTTTCAGACGc  >  1:45991/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    ACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTATCTGTGGGTGAAGTGGCAAAAGCAGCGGGCACGCCGTGGCTTACGGTGCTCGCGTTTGTCATTGGCGGGTTAATTGTGATCCCGCAAATGTGCGTCTATGCGGAACTATCCACCGCTTATC‑CGGAAAATGGCGCAGATTATGTTTATCTGAAAAATGCCGGAAGCCGACCGCTGGCTTTCCTCTCCGGCTGGGCCAGCTTCTGGGCCAACGATGCGCCGTCATTGTCGATTATGGCGCTGGCGATTGTCAGCAATCTTGGCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGTTTCAGACGC  >  NC_000913/3499882‑3500372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑