Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,481,131	G→A	L11L (CTG→TTG)	kefG ←	glutathione‑regulated potassium‑efflux system ancillary protein KefG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,481,131	0	G	A	100.0%	35.1 / NA	13	L11L (CTG→TTG)	kefG	glutathione‑regulated potassium‑efflux system ancillary protein KefG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/3);  total (10/3)

CCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCTGGG  >  NC_000913/3480904‑3481369                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               ccGGTCCAGCCCCTCTTTCAGTAGCGCCGGGCTGCTATAGGCATAAAGGGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTCATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGCGCTGCGTGGCCGGATTAAGCAGTACACGGTTTGCCACCGAGTCCTGCGATTCCGGATGGGCATACAACAGCAAAACTTTCgctggctg                                                                                                                                                                                                                           >  2:16427/1‑249 (MQ=255)          ccACTCTTTCCGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTACAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATcc                                                                                                                                                                                                                >  2:210906/1‑251 (MQ=255)                      tAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTAt                                                                                                                                                                                                    >  1:2405/1‑251 (MQ=255)                                         gTATAAAGCGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGc                                                                                                                                                                                 >  2:162549/1‑251 (MQ=255)                                                                                                  cGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGATTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCAt                                                                                                                        <  1:165629/251‑1 (MQ=255)                                                                                                             aaTAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGtt                                                                                                             >  1:171682/1‑251 (MQ=255)                                                                                                                      aTCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATCATGATAAGTTAACATAGTc                                                                                                    >  1:95362/1‑251 (MQ=255)                                                                                                                                    cgTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGGATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTGTGGTTGTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGGCCATACAGTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCAc                                                                                      <  1:22219/251‑1 (MQ=255)                                                                                                                                       aaaGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCtt                                                                                   >  2:256653/1‑251 (MQ=255)                                                                                                                                                   cGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTACTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTtc                                                                       <  1:268845/251‑1 (MQ=255)                                                                                                                                                                                                    cACCGAGTCCTGAGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACCTTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATgcc                       >  2:185456/1‑250 (MQ=255)                                                                                                                                                                                                            ccTGCGATTCCGGATGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATgccaccgccacc              >  1:210291/1‑251 (MQ=255)                                                                                                                                                                                                                         aTGGGCATACAACAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCACCCGGAATGGATTTTGTGTATAGTCGCTGGTTTGGGCTACCATTGCGCCCGGTTAGGCAGCTCGCCCATACATTACATTATCATAATGCTAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGCTTTCTCCTCGTTAAAAATTCGTCGCGGCGAGCACGTCCTGCTGTCTAATGCCACCGCCACCCTCAACCCTgtg  >  2:195475/1‑248 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               CCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGATGCTGAAAGACAATCACCTCGTGCTCGCGCAGTAATGCCTGCTCACGGGGGATATCAATAAAAAAATCGGGATAGTGCGCGTAAAGGTCGTGCACGGTAACATTGCTGAGCTGCGTGGCCGGTTTAAGCAGTACCCGGTTTGCCACCGAGTCCTGAGATTCCGGATGGGCATACAGCAGCAAAACTTTCGCTGGCTGAGACATCATCCCCCTCCCGGTATGGTTTTTGTGTATAGTCGCTGTTTTGGGCTACCATTGCGCCCGGTGCGGCAGCTCGCCCATACATTACATTATCATAATGATAAGTTAACATAGTCTGAACATACGGCACCTTATGATTGTTTTCTCCTCGTTACAAATTCGTCGCGGCGTGCGCGTCCTGCTGGATAATGCCACCGCCACCATCAACCCTGGG  >  NC_000913/3480904‑3481369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑