| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,433,665 | C→T | intergenic (‑105/‑25) | smf ← / → def | protein Smf/peptide deformylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,433,665 | 0 | C | T | 95.5% | 64.8 / NA | 22 | intergenic (‑105/‑25) | smf/def | protein Smf/peptide deformylase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (7/14); minor base G (0/1); total (7/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTT > NC_000913/3433421‑3433869 | ccaaaTGTAAGCCCTTTTTGCTGAAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCTTATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCAGCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCATAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGa < 2:168041/248‑1 (MQ=255) aaTACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCAGTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTTTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCa < 1:257839/251‑1 (MQ=255) caccAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAg < 2:204371/248‑1 (MQ=255) atGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCAGCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTAc < 2:84044/250‑1 (MQ=255) cGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCACTCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGACTAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACatat > 1:97477/1‑251 (MQ=255) tGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGCTTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCg > 2:188000/1‑251 (MQ=255) tttGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGa < 1:91513/250‑1 (MQ=255) gATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCCGAGGGGGATTTGTCTAGTATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGt > 1:267838/1‑251 (MQ=255) gACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTa > 2:92918/1‑250 (MQ=255) cATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAAcc < 1:92918/251‑1 (MQ=255) catcGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTa < 1:63115/250‑1 (MQ=255) catcGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATCTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTag < 1:234918/251‑1 (MQ=255) cGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCGTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTCGCTAAACCGGTAGaagaag < 1:269447/251‑1 (MQ=255) aGCTGCTGATACTAATTAAACGCAGCAAAATGTCTGTAGCGACTATCCTTATCTCCCTGTCAGAAGCAGCCTTAGCAATCTTTGCGAGTGGTCAGTGATGCGGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGGTTTGCAAGTGTTACTTATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATg < 1:175142/251‑1 (MQ=255) aCTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTc < 1:32379/250‑1 (MQ=255) cctCCTTATCACCCTGCCGTAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGGCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATTTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGTTAGAAGAAGTGAATGCTGAAATTCAGCGTATCGTCGATGATATTTTCGAGACGATGTa < 2:113248/249‑1 (MQ=255) aTCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTGGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAAGTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCTGTAGAAGAAGTGAATGGAGAAATTCAGCTTATCGTCGATGATATGTTCGAGACGATGTAc < 2:97477/251‑1 (MQ=255) cAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAAccc > 1:2123/1‑251 (MQ=255) ttGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGtt < 1:133343/250‑1 (MQ=255) cAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCa > 1:87158/1‑250 (MQ=255) cAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGCAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGtt > 2:226497/1‑251 (MQ=255) cAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCg < 1:109306/127‑1 (MQ=255) | CGCAATGTAAGCCCTGTTTGCTGCAATACAACCGCATCAATATGCGACTGTTTTGCCACCCAGTGAGCGATACGGACCATATCATCGCCGTACAAGCTGCTGATACTCATTAAACGCAGCCAAATTTCTGTATCGACCATCCTTATCTCCCTGCCATAAGCAGCCTTAGCAATCTTTGCGATTGGTCAGTGATGCTGTCAATCAGAGGGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTT > NC_000913/3433421‑3433869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |