Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,411,689	G→T	E13* (GAA→TAA)	yhdJ →	DNA adenine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,411,689	0	G	T	100.0%	61.9 / NA	26	E13* (GAA→TAA)	yhdJ	DNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/18);  total (8/18)

GAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTAACCAGACCCGTGCTGCGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATG‑CGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATC  >  NC_000913/3411445‑3411934                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       gAACAGCCCCTGTTGGACATGGTGAAGCAATACACCAGTTGTAACCAGACCCGTGCTGCGTTGATGATGGGCATCAACCGTTGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGGATGTTAACCGACCCGGTTTGGTAATTAAGCTa                                                                                                                                                                                                                                                  <  2:228060/251‑1 (MQ=255)  aaCAGCCCCTGTGTGACATGTTGATGCAATACACCCGTGGTAACCAGACCCGTGCTGCGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAATTAAGTTaa                                                                                                                                                                                                                                                 <  2:151373/251‑1 (MQ=255)               ggACATGGTGATGCAATACACCCGTGGTAACCAGACCCGTGCTGCGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGTATGTGAACCGACCCGGTTTGGTAATTAAGCTAAGACCATTATTCAc                                                                                                                                                                                                                                    <  1:42536/251‑1 (MQ=255)                                        ggTAACCAGACCCGTGCTGCGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACACGGTTTGGTAATTAAGCTAAGACCATTATTCACGGTGATG‑CGCTTGCCGAACTTaaa                                                                                                                                                                                                           >  2:147110/1‑250 (MQ=255)                                           aaccagctccGTGCTGCGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAATTAAGCTAAGGCCATTAGTCACGGTGATG‑CGCTTGCCGAACTTAAAAAg                                                                                                                                                                                                        <  1:211675/242‑1 (MQ=255)                                              cAGACCCGTGCTGCGCTGGTGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAATTAAGCTAAGACCATTATTCACGGTGATG‑CGCTTGCCGAACTTAAAAAGATcc                                                                                                                                                                                                    >  1:186913/1‑251 (MQ=255)                                              cAGACCCGTGCTGCGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAATTAATCTAAGACCATTATTCACGGTGATG‑CGCTTGCCGAACTTAAAAAGATcc                                                                                                                                                                                                    <  1:143583/251‑1 (MQ=255)                                                               gaggatgGGAATAAACCGAGGGACGCTGGGTAAAAAAGTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATAAAAAAGGCGATACGCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGGATGAGAACCGACCCGGTTTGGTAATTAAGCTAAGACCATTATTCACGTTGAGG‑CGCTTGCCGAACTTAAAAAGAGCCCCGCCGAAAGTGTCg                                                                                                                                                                                     <  2:66158/246‑1 (MQ=255)                                                                atgatgGGAATCAACCGTGGAACGATGCGTAAAAAATTGCAAAAATAAGGCATGAACTCATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCAAAAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAATTAAGCTAAGACCATTATTCACGGTGATG‑CGCTTGCCGAACTTAAAAAGATCCCAGCCAAAAGTGTCgatc                                                                                                                                                                                  >  2:27862/1‑251 (MQ=255)                                                                  gatgGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAATTAAGCTAAGACCATTATTCACGGTGATG‑CGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCgatctg                                                                                                                                                                                >  1:36360/1‑251 (MQ=255)                                                                              ccGTGGTACTCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATGCAGGTTAGCTAAATGTTTGATTAAAAAGGCGTTCCTCGGCATGGGTAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTTACCATG‑AGAACAGGATTTGAACCGACCCGGTTTGGTAATTAAGCTAAGACCATTATTCACGGTGATG‑CGTTGGCCGAACGTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGAc                                                                                                                                                                    <  2:228405/251‑1 (MQ=255)                                                                                         ggCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCAAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGCCACAAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAATTAAGCTAAGACCATTATTCACGGTGATG‑CGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATaa                                                                                                                                                         <  1:120124/250‑1 (MQ=255)                                                                                             aaaaaaaTTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACCCGGCATGGGGAAGCGCTTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAATTAAGCTAAGACCATTATTCACGGTGATG‑CGCTGGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATc                                                                                                                                                     <  1:55471/250‑1 (MQ=255)                                                                                                           aaTACGGCATGAACTAATTCAGGTTAGCTAAATGTTTTATTAAAAAGGCGTTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTTACCACG‑AGAACATGATGTGACCCGTCCCGTTTTGGTAATTAAGCAAAGACCATTATTCACGGTGATG‑CGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGa                                                                                                                                       <  2:228108/251‑1 (MQ=255)                                                                                                                               aGGTTAGCAAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAATTAAGCTAAGACCATTATTCACGGGGATG‑CGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGa                                                                                                                   <  1:182278/251‑1 (MQ=255)                                                                                                                                                    aaaaaGGCTCTGCTCGGCTGGGGGGAGTGCCTTTTTGATAGGTGTCACAAAGGGAGTTAC‑ATGGAGAACAGGATGTGAGCCGACCCGGTTGTGTAATTAAGCTAGGACCTTTTTTCACGG‑GTTGGCGCGTGGCGAGCTTAAAAAGACCCCCGTCGAAAGTGTCGATCTGATCTTTGCCGGCCCACCGTATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCAGGAAACAAGATCGGTTTATTGACt                                                                                              <  2:102725/251‑1 (MQ=255)                                                                                                                                                                    gCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAATTAAGCTAAGACCATTATTCACGGTGATG‑CGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGAt                                                                              >  1:70619/1‑251 (MQ=255)                                                                                                                                                                      agggggAAGCGCCTTTTTTATAGTGGTCACAAAGGGAGTGCCCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAAGTAAGCTAAGACCTTTATTCACGGTGATG‑CGCTTGCCGAGTTTAAAAATATCCCCGCCGAAAGTGTCGATCGGATCTTTGCCGACCCACCATATAACATCTGTAAAAATTTTGATGGTCTGATGGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTg                                                                            <  1:264591/249‑1 (MQ=255)                                                                                                                                                                              gcgcCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAATTAAGCTAAGACCATTATTCACGGTGATG‑CGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGc                                                                    >  2:86788/1‑251 (MQ=255)                                                                                                                                                                                gcCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACCGGATGTGAACCGACCCGGTTTGGTAATTAAGCTAAGACCATTATTCACGGTGATG‑CGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGccc                                                                  >  2:201796/1‑250 (MQ=255)                                                                                                                                                                                                   caAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAATTAAGCTAAGACCATTATTCACGGTGATG‑CGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCGGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAg                                               <  1:83290/251‑1 (MQ=255)                                                                                                                                                                                                                 aTG‑AAAACAGGAGGGGAACCGACCCTGTTTGGAAATTAAGCTAAGACCATTATTCACGGTGATG‑CGCTGGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTTGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTAca                                  <  2:150382/250‑1 (MQ=255)                                                                                                                                                                                                                             aTGTGCACCGACAAGGTTTGGTAATTACGCTAAGACCATTATTCACGGCGATC‑AGATCGCCGAGCATAACAAGATACCCACCGAAAGTGTCGATCTGCTCTTTCCCGCCCCACCAACTAACATCGGTACAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGCTTGCAGAGTGGAACCGCGTTCTGAAAAAGCAGGGCAGCAAGAACATCATGaccc                         >  2:139972/1‑245 (MQ=255)                                                                                                                                                                                                                               gtgAACCGCCCCGGTTTGGTAATTAAGCTAAGACCATTATTCCCGGTGATG‑CGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACg                    <  1:27558/251‑1 (MQ=255)                                                                                                                                                                                                                               gtgAACCGACCCGGTTTGGTAATTAAGCTAAGACCATTATTCACGGTGATG‑CGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACg                    <  1:86788/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gTAATTAAGCTAAGACCATTATTCACGGTGATG‑CGCTTTCCGAACTTAAAAAGTTCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTTGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATc  <  2:36360/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       GAACAGCCCCTGTTGGACATGGTGATGCAATACACCCGTGGTAACCAGACCCGTGCTGCGCTGATGATGGGCATCAACCGTGGTACGCTGCGTAAAAAATTGAAAAAATACGGCATGAACTAATTCAGGTTAGCTAAATGCTTGATTAAAAAGGCGCTACTCGGCATGGGGAAGCGCCTTTTTTATAGGTGTCACAAAGGGAGTGACCATG‑AGAACAGGATGTGAACCGACCCGGTTTGGTAATGAAGCTAAGACCATTATTCACGGTGATG‑CGCTTGCCGAACTTAAAAAGATCCCCGCCGAAAGTGTCGATCTGATCTTTGCCGACCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGCCCTTTATC  >  NC_000913/3411445‑3411934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑