Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	78.7 / NA	29	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/13);  total (16/13)

GATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGT  >  NC_000913/3387799‑3388271                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       gATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACcggcgg                                                                                                                                                                                                                                <  1:58674/251‑1 (MQ=255)  aTCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCACTAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCggcggc                                                                                                                                                                                                                               >  1:76125/1‑251 (MQ=255)  aTCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCggcggc                                                                                                                                                                                                                               >  1:144799/1‑251 (MQ=255)                                gCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCg                                                                                                                                                                                                 >  2:227754/1‑251 (MQ=255)                                            tCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCGATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGAACCGCCCAGCGTGGCGTTTcc                                                                                                                                                                                       >  1:104463/1‑249 (MQ=255)                                                          cGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGc                                                                                                                                                                       >  2:50602/1‑251 (MQ=255)                                                                           aaaaaagggCCCCGATACAGCACCCAAGAATCATCAATAATGGAGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATAAAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGACTACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGTAAGCCAACAACCAGCGcc                                                                                                                                                         >  2:247202/8‑248 (MQ=255)                                                                               aCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACaa                                                                                                                                                  <  1:51647/251‑1 (MQ=255)                                                                                      cAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGGACAATGGCGGt                                                                                                                                           <  1:227754/251‑1 (MQ=255)                                                                                       aGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCgggc                                                                                                                                          >  2:8145/1‑249 (MQ=255)                                                                                            cAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGACGTGAAACCCAACAAACAGCGCCAGTACAATGGCGGgcgca                                                                                                                                       >  2:140169/1‑248 (MQ=255)                                                                                                     aGAATTATAAATAATGGGGCGCGGATCATCGCAATGAGGATCACAAGGCCGGCAATACAGCCAAAAAAGGGGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTTGCGGTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCTAGTTTTACCGc                                                                                                                            <  2:226615/251‑1 (MQ=255)                                                                                                           atcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCAGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCg                                                                                                                      <  2:41218/251‑1 (MQ=255)                                                                                                                taatGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCACTAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGACTAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCCGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTAGCCCGTTTTAcccgataggaagcgc                                                                                                                 >  1:259978/1‑238 (MQ=255)                                                                                                                gaatGGTGCGCGGATCATCGCAATGATGATCACCAGGCCGGCAATACAGCCAATAAATGTGCCGATGAGGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGGGTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTGTTAcc                                                                                                                              <  2:259978/237‑1 (MQ=255)                                                                                                                  atGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCACAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACCGCGCCAGTACAATGGAGGATGCCAGTTTTTCACCAAAGCGAATATGt                                                                                                               >  2:93898/1‑251 (MQ=255)                                                                                                                                    cAATGATGATCAACAGTACGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGTCCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGaaa                                                                                             <  1:213972/251‑1 (MQ=255)                                                                                                                                                  aGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGACAGCca                                                                                                                                                                     >  2:221395/1‑165 (MQ=255)                                                                                                                                                  aGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCca                                                                                                                                                                     <  1:221395/165‑1 (MQ=255)                                                                                                                                                        gCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACCGCGCCAGTACAATTGCGGTACCCCGTTTTACCGCAAAGCGGATATGATGGGTAGCAATGGGGAAAAAAACCAACGTGAATAAcc                                                                          >  2:30426/1‑250 (MQ=255)                                                                                                                                                                       aaTGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTAGCCACTTTTACCCCAAAGCGAATATGGTGGTTAGCAATTGAGGAAAAACCCCTCGTGAtatacccaaatccccccggcg                                                           >  2:198616/1‑230 (MQ=255)                                                                                                                                                                                       gcAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTTCCGCAAAGCGAATATTTTGGGTTGCAATGGAGGAAAAACCCACCGGGATTAAACAAACTCACGCAGGGGCTGGGCCCTTTTACggg                                          >  2:88465/1‑250 (MQ=255)                                                                                                                                                                                                     tAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACACACAGCGCCAGTACAATGGCGGTCGCCAGGTTTACCGCAAAACGAATATGTTTGTTAGCAATGGGGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGGTGGGCCATTTTACGGAAGAAAGAATCCTgg                            >  1:242904/1‑251 (MQ=255)                                                                                                                                                                                                      aGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGGCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGc                           <  1:33013/251‑1 (MQ=255)                                                                                                                                                                                                        cGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCtt                         <  2:235842/251‑1 (MQ=255)                                                                                                                                                                                                          aaTAGCGCCAGAATCCGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGGTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTtc                       <  1:140169/251‑1 (MQ=255)                                                                                                                                                                                                                      aTACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTg           <  1:177356/251‑1 (MQ=255)                                                                                                                                                                                                                       tACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTAGCGGTCTTGt          >  1:195433/1‑251 (MQ=255)                                                                                                                                                                                                                               aCCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGt  <  1:8145/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                       GATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCACGCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGT  >  NC_000913/3387799‑3388271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑