Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,329,743	G→T	D261Y (GAT→TAT)	dacB →	peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,329,743	0	G	T	96.9%	96.4 / NA	32	D261Y (GAT→TAT)	dacB	peptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (18/13);  total (18/14)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGG‑TAGTTGCC‑AGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCT  >  NC_000913/3329512‑3329981                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 ccTGGTGATATGGCTGTTATACGCGTGGCATCTTATTACCCCTTTACGATGTTCAGCCAGGTACGCTCCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCggg                                                                                                                                                                                                                               <  1:54967/251‑1 (MQ=255)              cTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAgg                                                                                                                                                                                                                  >  1:262272/1‑250 (MQ=255)                     aCGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGGGCGGAacac                                                                                                                                                                                                           >  1:59481/1‑251 (MQ=255)                                      aCCCCGTTACGAGGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAg                                                                                                                                                                                          <  1:213454/251‑1 (MQ=255)                                          cGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGGGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAGATATGAGTTACAACAGGCGGGTATTACCTGGAGCCGAAAACTGATGAGCCaaaatcaagtg                                                                                                                                                                                       >  1:226983/1‑240 (MQ=255)                                                           aGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAGCAGGCGGGGATCACCTGGAGCGGAACACTGCTGCCCCAAACTCCGGGTTACGAACCTGGTACGG‑t                                                                                                                                                                     >  2:124781/1‑250 (MQ=255)                                                                           ccggggTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGG‑TAGTTGCC‑AGTAAACAGt                                                                                                                                                   <  1:135517/247‑1 (MQ=255)                                                                                  tCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTAACACCTGGTGGGGAAAACAGCTGCGCCAGACTCAGGATAAAGAAACTGGAACGGGTA‑TTTCC‑AGTAAACAGTAGGcccc                                                                                                                                            >  2:64443/1‑251 (MQ=255)                                                                                       cGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGG‑TAGTTGCC‑AGTAAAAAGTCGGCCCCGCTGc                                                                                                                                       >  1:259489/1‑251 (MQ=255)                                                                                              cAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGG‑TAGTTGCC‑AGTAAACAGTCGGCCCCGCTGCACGATCt                                                                                                                                <  2:59481/251‑1 (MQ=255)                                                                                              cAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCATGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGGGGAAAACAGCTGCGCCAGACACAGGTTAACGAAACCGGAACGG‑GAGGTGCA‑AGTAAACAGACGGGCCCGcggccagggct                                                                                                                                >  2:260359/1‑241 (MQ=255)                                                                                               aaTACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTAAGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTAGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTAACAACAGGGGCGGAAAAATGCTGCGCCAGACCCCGGTTAAAGAACCTGGAACGG‑TAGTTGCCCAG‑AAAAAGTCGGCCCCGCTTCACGaattg                                                                                                                               >  1:135412/1‑247 (MQ=255)                                                                                                                 tggtgCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAAACTGGAACGG‑TAGTTGCC‑AGGAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGaa                                                                                                              >  1:243380/1‑250 (MQ=255)                                                                                                                      ccAGGCGACCTGACCCGCGTTACGCTGACGGGTTGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTTAAATATGAGTTAAAACAGGCGGGTATCTCCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTTGATCGG‑TAGTTTCT‑AGTAAACAGGCGGCCCCGCTTCACGATCTTCTTAATATTTTTCTGAAAAAGTc                                                                                                        <  2:265767/251‑1 (MQ=255)                                                                                                                        aGGCGACCGGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGATCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCTCCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTTGAACGG‑TAGTTGCC‑AGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCgg                                                                                                      <  1:254363/251‑1 (MQ=255)                                                                                                                          gCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACTCTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGG‑TAGTTGCC‑AGTAAACAGTCGGCCCCGCTGCACGAGCTGCTTAAGATTATGCTGAAAAAGTCGGac                                                                                                    <  1:117645/251‑1 (MQ=255)                                                                                                                                          tACGCTGTCGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGCACACTGCTGCGCCAGACTCAGGCTAACGAACCTGGAACGG‑TAGTTGCA‑CGGCAACAGTCGGCCCCGCCGCCCGATCTCCTTAAGAATCTGTTGAAAAAATCGGACAACATGATCCCCgct                                                                                     >  2:93230/1‑248 (MQ=255)                                                                                                                                               tGACGGGATGCCTGACACAACGTTCTGAGACGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGG‑TAGTTGCC‑AGGAAACAGTCGGCCCCGCTGCCCGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATAAGGtt                                                                               >  1:219083/1‑251 (MQ=255)                                                                                                                                                gACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGTTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGG‑TAGTTGCC‑AGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGttt                                                                              <  2:259489/251‑1 (MQ=255)                                                                                                                                                            gccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGG‑TAGTTGCC‑AGGAAACAGTCGGCCCCGCTGCACGAACTGCTTAAGATTATGCTGAAAAAGTCGGACAACCTGATCGCCGATACGGTTTTCCGCATGAt                                                                    >  1:79138/1‑249 (MQ=255)                                                                                                                                                                 aaCGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGACAACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGG‑GAGTTTCC‑AGTAAACAGTCGGCCCCGCTGCCCGGTATGCATCAGATTCTGCTGAAAAAGTCGGACAACCTGTTCGCCGCTACGGTTTTCCGCATGATAGGCCAt                                                             >  2:215643/1‑251 (MQ=255)                                                                                                                                                                          aGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGG‑TAGTTGCC‑AGTAAACAGTCGGCCCCGCTGCACGATCTg                                                                                                                               <  2:129434/176‑1 (MQ=255)                                                                                                                                                                          aGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAAACTGGAACGG‑GAGTTGCC‑AGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTTAAAAAGTCGGACAAAATGATCGCCGATACGGGTTTCCGCATGATAGGCCATGCGCGCttt                                                    >  1:206485/1‑250 (MQ=255)                                                                                                                                                                          aGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGGTAACGAACCTGGAACGG‑TAGTTGCC‑AGTAAACAGTCGGCCCCGCTGCACGATCTg                                                                                                                               >  1:129434/1‑176 (MQ=255)                                                                                                                                                                                           tttGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGG‑TAGTTGCC‑AGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACAt                                    <  1:209260/250‑1 (MQ=255)                                                                                                                                                                                                        aTGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTTGAGCGGAAACCTGCTGCGCCAGACTCAGGTTAAAGAACCTGGGACGG‑AAGGTGCC‑AGTAAACAGTCCGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGGTACGGTTTTTCCCATGATAGGCCCTGCGAGCGTCAATGGGCCAGGAACAAGGCGGGcgcggtcg                      >  2:207280/1‑244 (MQ=255)                                                                                                                                                                                                          ggAGCAAGCTAAGCCCGTGCAATTCTGAAATAGGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTCGGCCAGACGCAGGTTAACGAGCCTGGAAATG‑TTGTTGCC‑AGTAAACAGGCGGCCCCGCTGCACGATCTGCTTATGGTTATGCTGAAAAAGTCGGGAAACATGATCGCCGATCCGGTTGTCCGCATGATAGGCCGTGCGCGCTGCAATGTTCCTGGAACAtcgtgggtcgcgtctga                    <  2:71271/251‑17 (MQ=255)                                                                                                                                                                                                           gAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGGGCGGAACAATGCTGCGCAAGACTCAGGTTAACGAACCTGGGACGG‑GAGTTGCC‑AGTTAACAGTCGGCCCCCCCGCCCGAACCGCTTTAGATTATGCTGAAAAAGTCGGGAAACATGATCGCCGATACGGTTTTCCGCATAATAGGCCCTGCGCGCTTAGAAGTAGCTGGTACATGGCGGGCCggggggggg                   >  2:209462/1‑245 (MQ=255)                                                                                                                                                                                                              ccAGCTATGCCGGTGCAATTCTGAAATATGAGTGAAAACGGTCGTGTATCGCCTGGAGCGGAACACTGCGGCGCCAGACGCAGGTTAACGAACCGGGAACGG‑TAGTTGCC‑AGTAAACAGTCTGCCCCGCTGCACAATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCTATACGGGTTTCCGCATGATAGTCCATGCGCGCTTCCAGATGCCTGGATCACGGCGAGCCGGGACGGACGcc                <  2:16861/251‑1 (MQ=255)                                                                                                                                                                                                                 gCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGG‑TAGTTGCC‑AGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGGTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGGCGGACGCCggg             >  1:94592/1‑249 (MQ=255)                                                                                                                                                                                                                 gCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACGCAGGTTAACGAACCTGGAACGG‑TAGTTGCC‑AGTAAACAGTCGTCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACATCATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTg             <  1:18108/251‑1 (MQ=255)                                                                                                                                                                                                                            gCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGG‑TAGTTGCC‑AGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCt  <  2:243380/251‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 CCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGG‑TAGTTGCC‑AGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCTGAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCT  >  NC_000913/3329512‑3329981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑