Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,279,276	C→G	F121L (TTC→TTG)	kbaZ →	putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,279,276	0	C	G	100.0%	93.5 / NA	30	F121L (TTC→TTG)	kbaZ	putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (13/17);  total (13/17)

AACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTG‑AACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGGCGGTCACCACGCCGGATGCCGCCCGCGCCACGCTGGAAGC  >  NC_000913/3279031‑3279521                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       aaccaaacGCCGTAATTGATTGAAGCACCCTCCAAGCAGGTAGACCAGTTCGGGGGTTATACCGGAATGCCGCCCGCCGATTTTCGCGGCGTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGAGGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGaaaaa                                                                                                                                                                                                                                                   <  2:195959/251‑1 (MQ=255)    caaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGGATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGACTTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCCAAACCTGCCGGCCGCTCAGGCAAAGGCCCATGCCGATGATTTGCTTACAAACTACGTTTCGGCAGGATTGaaaaaaa                                                                                                                                                                                                                                                 >  1:101403/1‑250 (MQ=255)         gCCGTTACTGATTGAAGCAACCCCCAATCGGGTAGACCAGTTCGGCGGTTATACCGGAAGGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCAcc                                                                                                                                                                                                                                           <  1:222518/251‑1 (MQ=255)              tccggATTGAAGCACCCCCCAACAAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCTGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGa                                                                                                                                                                                                                                       <  2:188959/246‑1 (MQ=255)                     tgagccatccTCCAATCAGGTGTACCAGTCCGGTGTTTAGTCCGGAATGACCCCCGCCTATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGTCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTgcagc                                                                                                                                                                                                                                <  2:118178/242‑1 (MQ=255)                       aaccaccccccAATCAGGTAGACCAGTTCGGCGGTTATCCCGGAAGGCCCCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATg                                                                                                                                                                                                                             <  2:204996/242‑1 (MQ=255)                          cAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTcc                                                                                                                                                                                                                          >  1:89292/1‑251 (MQ=255)                                     cAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCCTTGTTTGTCAGCTCGCCGGCTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGGTTGAAAAAAATCCCCCCTGATTGCCGCatgtcatgtctgggcg                                                                                                                                                                                                                >  2:122347/1‑244 (MQ=255)                                                gTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCACTGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAAACCACCCTGATTTCAGGATGTCCTGTACGGGCGTTCCGATTCCCt                                                                                                                                                                                                    >  2:211160/1‑251 (MQ=255)                                                 gccggggttttccccGGAAGGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCAGCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGGTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCtt                                                                                                                                                                                                   <  2:168774/238‑1 (MQ=255)                                                   gggcggTTATACCGGAAGGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCATGTCAGGACGATCCGATTCCCTTaa                                                                                                                                                                                                 <  1:122347/250‑1 (MQ=255)                                                       ggTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAACACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCCCCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTaaatgt                                                                                                                                                                                             >  2:173053/1‑247 (MQ=255)                                                               cGGAATGACGCAAGCCGATTTTCGCGGCTTTGTTTGTCAGATCGCCGACTCGTTGAATTTCCCGCAGGCTGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACATGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCAAGTCCTGTAAGGACGATCCGATTCCCTTAACTGCTGACATCg                                                                                                                                                                                     >  2:194419/1‑251 (MQ=255)                                                                     gACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTg                                                                                                                                                                               <  1:44719/251‑1 (MQ=255)                                                                        gcccgccGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTAAACTGATGACATCGTGGCTGAAc                                                                                                                                                                            <  1:77093/251‑1 (MQ=255)                                                                                             tgtttgtCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTTATTATGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGCTGCTTTGATTAAAAGCTACGTTGCGGCCGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGCTTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGaa                                                                                                                                                         >  1:113672/1‑249 (MQ=255)                                                                                                       cTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGga                                                                                                                                                >  1:16103/1‑248 (MQ=255)                                                                                                            cGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAAGGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACAGCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCt                                                                                                                                        <  2:101403/251‑1 (MQ=255)                                                                                                                tCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGCACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAAAAACCTGTCt                                                                                                                                    >  2:64673/1‑251 (MQ=255)                                                                                                                           cccGAAGGAGGCGGTGGTTCTGGGTGGTGACCATCTGGGGCCAAACCGCGGGTAAAACCTGGCGGCCGCTCAGGCAAGGGCCAATGCCGATGATTTGATTAAAAGCTACGTGGCGGCAGGATTGAAAAAAAGCCACCTTGATTGCAGGATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAAGGCGCCGCCGGTCTGGCGAAAGTGGCGgaagaagtccggcttgagcgctttg                                                                                                                         <  2:113672/251‑20 (MQ=255)                                                                                                                                aGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTG‑AACACTTTGGCGaa                                                                                                                   <  1:32852/251‑1 (MQ=255)                                                                                                                                                          cATCTGGGGCCAAACCGCTGGCAAAACATGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGGTTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGACAGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGGAACCTGTCTTGGAAC‑CTTTGGCGAAGACGGTCTGGGGAATGTCCTTgggat                                                                                         >  2:231267/1‑248 (MQ=255)                                                                                                                                                                               cAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGCTTTGATTAAAAGCTACGTTGCGGCAGGATTGCAAAAAATCCACCTTGCTTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGCTGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTT‑GACACTTTTGCGAAGCCGCTCTggcggtttt                                                                                                  >  2:35852/1‑214 (MQ=255)                                                                                                                                                                               cAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTG‑AACACTTTGGCGAAGCCGATCTGGAGTATGt                                                                                                  <  1:35852/221‑1 (MQ=255)                                                                                                                                                                                                           ccAATGCCGATGAGTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGGGGCTGAACGCGCCGCCCGGCTGGCGAAAGTGGCGGAAGAAACCTGTCTTG‑AACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGGCg                                        <  2:98402/251‑1 (MQ=255)                                                                                                                                                                                                                   gatgatTTGATTAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTG‑AACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGGCGGTcaccac                                <  2:89292/251‑1 (MQ=255)                                                                                                                                                                                                                       atttgattAAAAGCTACGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTG‑AACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAATCGAGCTGGCGGTCACCACGCCg                            <  1:215444/251‑1 (MQ=255)                                                                                                                                                                                                                                       cGTTGCGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCCGTCTTG‑AACACTTTGGCGAAGCCGATCTGGGGGATGTCAATGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGGCGGGCACCACGCCGGATGCCGCCCCCGccc            >  1:204469/1‑250 (MQ=255)                                                                                                                                                                                                                                            cGGCAGGATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTG‑AACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGGCGGTCACCACGCCGGATGCCGCCCGCGCCACGCTg       <  1:64673/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ggATTGAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTG‑AACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGGCGGTCACCACGCCGGATGCCGCCCGCGCCACGCTGGAAGc  >  1:54289/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       AACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTGGGTGGTGACCATCTGGGGCCAAACCGCTGGCAAAACCTGCCGGCCGCTCAGGCAATGGCCAATGCCGATGATTTGATTAAAAGCTACGTTGCGGCAGGATTCAAAAAAATCCACCTTGATTGCAGCATGTCCTGTCAGGACGATCCGATTCCCTTAACTGATGACATCGTGGCTGAACGCGCCGCCCGTCTGGCGAAAGTGGCGGAAGAAACCTGTCTTG‑AACACTTTGGCGAAGCCGATCTGGAGTATGTCATTGGTACCGAAGTGCCGGTACCTGGCGGCGCGCATGAAACCTTAAGCGAGCTGGCGGTCACCACGCCGGATGCCGCCCGCGCCACGCTGGAAGC  >  NC_000913/3279031‑3279521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑