Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,965,617	C→T	A141T (GCC→ACC)	lgt ←	phosphatidylglycerol‑‑prolipoprotein diacylglyceryl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,965,617	0	C	T	100.0%	39.1 / NA	14	A141T (GCC→ACC)	lgt	phosphatidylglycerol‑‑prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/9);  total (5/9)

ACAGGTTGAGGATAATAAACAGCACCACACCTTCCAGCAGCAGCTCGTAAAGCTGTGATGGGTGGCGCGGCAGCACACCGTAAGTGTCGAAAATGGATTGCCACTGCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCG  >  NC_000913/2965389‑2965850                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          aCAGGTTGAGGATAATAAACAGCACCACACCTTCCAGCAGCAGCTCGTAAAGCTGTGATGGGTGGCGCGGCAGCACACCGTAAGTGTCGAAAATGGATTGCCACTGCGGGTTGGTTTGCAGCAGCAAAATATCTTCGGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGTACCAAGACCAAACGGAATGAgt                                                                                                                                                                                                                     <  2:187678/251‑1 (MQ=255)     gTTGAGGATAATAAACAGCACCACACCTTCCAGCAGCAGCTCGTAAAGCTGTGATGGGTGGCGCGGCAGCACACCGTAAGTGTCGAAAATGGATTGCCACTGCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGATCGGGTCAACGCGGCCCCACAATTCACCGTTAATAACGTTGCCCAGACGCCCGGTACCAAGACCACACGGAATGAgtggtg                                                                                                                                                                                                                 >  1:190486/1‑251 (MQ=255)                                   cagcagcagCTCGTAAAGCTGTGATGGGTGGCGCGGCAGCACACCGTAAGTGTCGAAAATGGATTGCCACTGCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGTACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGATGGAAc                                                                                                                                                                                   >  1:69116/1‑251 (MQ=255)                                          agcTCGTAAAGCTGTGATGGGTGGCGCGGCAGCACACCGTAAGTGTCGAAAATGGATTGCCACTGCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGTACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGt                                                                                                                                                                            <  1:169174/251‑1 (MQ=255)                                                 aaaGCTGTGATGGGTGGCGCGGCAGCACACCGTAAGTGTCGAAAATGGATTGCCACGGCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGTACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCATAGACCTGGAAGAAGGAACGTTTAGTAcggcgg                                                                                                                                                                     <  1:188967/251‑1 (MQ=255)                                                                            cacCGTAAGTGTCGAAAATGGATTGCCACTGCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGTACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACg                                                                                                                                          <  1:171459/251‑1 (MQ=255)                                                                                ggaaGTGTCGAAAATGGAGTGCCACTGCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGTACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTTGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCac                                                                                                                                      <  2:18988/249‑2 (MQ=255)                                                                                    gtgtCGAAAATGGATTGCCACTGCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGTCCCACAATGCACCGTTAATAAAGTTGCCCAGACGCCCGGTACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAg                                                                                                                                  <  1:137360/251‑1 (MQ=255)                                                                                           aaaTGGATTGCCACTGCAGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGTACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGGAGATAATCATcacccacagaaccccaacaaagccgccg                                                                                                                           >  1:94719/1‑227 (MQ=255)                                                                                              tGGATTGCCACTGCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGTACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGGAGATAATCATCACCACGATAAAGcccatacgggcgccgggg                                                                                                                        >  1:68299/1‑235 (MQ=255)                                                                                                    gCCACTGCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGTACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGGAGAAAATCATCCACACGATAAAGCCAAACCAGCCGCCGTgggaaaac                                                                                                                  >  1:90119/1‑245 (MQ=255)                                                                                                                                                                                           ggCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGTACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATa                                                                                     <  1:33131/193‑1 (MQ=255)                                                                                                                                                                                           ggCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGTACCAAGACCAAACGGAATGAGTGGTGCGATAAAAACAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGGCCCAGACACGGAACAGATACAGCGGATCGGCCATATACTGCGGGAAATTGTAGAACAGAACATAACCATTCCGTccc                           <  2:48292/251‑1 (MQ=255)                                                                                                                                                                                                                    gTTGCCCAGACGCCCGGTACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCg  <  1:29804/251‑1 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          ACAGGTTGAGGATAATAAACAGCACCACACCTTCCAGCAGCAGCTCGTAAAGCTGTGATGGGTGGCGCGGCAGCACACCGTAAGTGTCGAAAATGGATTGCCACTGCGGGTTGGTTTGCAGCAGCAAAATATCTTCTGTACGGGAGCCAGGGAACAGCATGGCAAACGGGAAGTTCGGGTCAACGCGGCCCCACAATTCACCGTTAATAAAGTTGCCCAGACGCCCGGCACCAAGACCAAACGGAATGAGTGGTGCGATAAAATCAGAGACCTGGAAGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCG  >  NC_000913/2965389‑2965850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑