Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,955,316	C→T	D230N (GAT→AAT)	recB ←	exodeoxyribonuclease V subunit RecB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,955,316	0	C	T	100.0%	111.1 / NA	34	D230N (GAT→AAT)	recB	exodeoxyribonuclease V subunit RecB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/15);  total (19/15)

GATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCT  >  NC_000913/2955082‑2955558                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   gATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAAtt                                                                                                                                                                                                                                    >  1:167840/1‑251 (MQ=255) gATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCGGGGAGAATGTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATGCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAAtt                                                                                                                                                                                                                                    <  2:165656/251‑1 (MQ=255)    gTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTgc                                                                                                                                                                                                                                 <  1:33343/251‑1 (MQ=255)        cgGGGTTTCCCCCCCGGCCTTCTTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTGGCGCGt                                                                                                                                                                                                                             <  2:54254/251‑1 (MQ=255)                 ccccccGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTGTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTTTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAGCTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCGCCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCTTGACGGGAAg                                                                                                                                                                                                                    <  1:44997/251‑1 (MQ=255)                              gTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTtcatc                                                                                                                                                                                                       >  1:9575/1‑251 (MQ=255)                                          aaGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACGGCGGCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCTGGcggcgg                                                                                                                                                                                           <  2:71296/251‑1 (MQ=255)                                                    gggAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGa                                                                                                                                                                                   >  1:140347/1‑249 (MQ=255)                                                    gggAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGa                                                                                                                                                                                   <  2:140347/249‑1 (MQ=255)                                                                     gACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTg                                                                                                                                                                <  2:9575/251‑1 (MQ=255)                                                                                    tAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATCCCAGAAGCTTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTatt                                                                                                                                                 >  2:112604/1‑249 (MQ=255)                                                                                         gTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATcgc                                                                                                                                            >  1:120428/1‑251 (MQ=255)                                                                                                 cttcttctGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTGGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCgcagca                                                                                                                                      <  1:22472/249‑1 (MQ=255)                                                                                                   tcttctGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGc                                                                                                                                  >  2:196992/1‑251 (MQ=255)                                                                                                     ttctGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCt                                                                                                                                >  2:2121/1‑251 (MQ=255)                                                                                                                 gcTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTcc                                                                                                                    <  1:2121/251‑1 (MQ=255)                                                                                                                  cTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAAca                                                                                                                                                                              <  2:252694/192‑2 (MQ=255)                                                                                                                  cTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAAca                                                                                                                                                                              >  1:252694/1‑191 (MQ=255)                                                                                                                     aTCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGt                                                                                                                >  2:92243/1‑251 (MQ=255)                                                                                                                            cGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCaa                                                                                                           >  1:194358/1‑249 (MQ=255)                                                                                                                                            aTTGCAACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTACAGGTTTAAAAGACGACCTGGGCTAttt                                                                                         >  2:238254/1‑251 (MQ=255)                                                                                                                                                                                  atgcGATCCGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCg                                                   <  2:167840/248‑1 (MQ=255)                                                                                                                                                                                  aTTCGATAAGCGCATCCAGATCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGACGCCCGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAAAGCCTGCGGCCCTTTCCAGGTTTCAAAGACGCCCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCg                                                   >  2:158064/1‑251 (MQ=255)                                                                                                                                                                                   ttCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCgg                                                  >  1:167450/1‑251 (MQ=255)                                                                                                                                                                                          aGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGc                                           <  1:19366/251‑1 (MQ=255)                                                                                                                                                                                           gcgcATCCAGTTCACCCACTGCGTCGCGCCACGGCTGTTTTACCGTATTATTACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGTTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGcc                                          <  1:176044/251‑1 (MQ=255)                                                                                                                                                                                                  cAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGgtagc                                   >  2:267044/1‑251 (MQ=255)                                                                                                                                                                                                    gTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGAAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACAGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTG‑CGCCGCCAGAACTCGGCGCAGGCCTGgaagcgt                                 >  1:12569/1‑250 (MQ=255)                                                                                                                                                                                                     ttCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGgtagcgt                                 >  1:158793/1‑250 (MQ=255)                                                                                                                                                                                                                 gTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGGCAGAAATCGGCGCAGGCCt                                         <  1:210175/230‑1 (MQ=255)                                                                                                                                                                                                                 gTCGCGCCACTGCTGTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCt                                         >  2:210175/1‑230 (MQ=255)                                                                                                                                                                                                                          cgtctgTTTTACCGTATTAATACGCGCCACAATGTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTATCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGCCGCCAGAAATCGGCGCAGGCCTTGTAGCGTAGCATATACTCATCTTCAATc            <  2:230635/247‑1 (MQ=255)                                                                                                                                                                                                                              tgTTTTACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAgctg       >  1:121235/1‑251 (MQ=255)                                                                                                                                                                                                                                   tACCGTATTAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATCACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGGGACGACGCCAGAAATCGGCGCAGGCCTGGTAGCGGAGCCGAGACTCACCTTCAATCAgctgctgcg  >  1:31246/1‑250 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   GATGTCGCGGGGTTTCCCCCCCGGCCTTCGTGCGATCTTCTAAGAAACGCTGGGAGAATTTTTCCAGCGACTCCGGCAACTGATAACTGTTTGTCTCTTCTTCTGCCCAGGCGCTGATCTTGTCGATCCATTTAGCCTGATTGCTACGGTTAAACTTGCGTCGATCAATACCAGAAGATTCGATCAGCGCATCCAGTTCACCCACTGCGTCGCGCCACTGCTGTTTTACCGTATCAATACGCGCCACAATTTGCGCGTGACGGGAAGCCAGCGTTTCATCATCGGGCGGCGGTGCTTTGATAACCGGCGCTTCGCCTTGCAGATAACGATTAATATCGCGCAGCAACGCCTGCGGCCCTTTCCAGGTTTCAAAGACGACCTGGGCTATTTCACGCGGCAGCGGGTAGCAGTGGCGACGCCAGAAATCGGCGCAGGCCTGGTAGCGTAGCAGAGACTCATCTTCAATCAGCTGCTGCT  >  NC_000913/2955082‑2955558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑