Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,924,310	C→T	noncoding (215/369 nt)	csrB ←	small regulatory RNA CsrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,924,310	0	C	T	100.0%	92.3 / NA	30	noncoding (215/369 nt)	csrB	small regulatory RNA CsrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/11);  total (19/11)

CGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCCGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTA  >  NC_000913/2924066‑2924556                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       cGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGAtgtg                                                                                                                                                                                                                                                  >  2:83195/1‑251 (MQ=255)                                    cATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGAc                                                                                                                                                                                                               >  2:242060/1‑251 (MQ=255)                                     aTGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACCCCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGAcc                                                                                                                                                                                                              >  2:234848/1‑251 (MQ=255)                                                  aaGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATcc                                                                                                                                                                                                 >  2:39699/1‑251 (MQ=255)                                                                              gAAGGATAGCAGGAATAAAAAAAGGGAGCACTGTATTCACCGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGACTCATCCTGACCGGTTCTCATTCTCCATCCTgg                                                                                                                                                                       >  2:83478/1‑249 (MQ=255)                                                                                     aGCAGGAATAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGCGGTGTCCt                                                                                                                                                              >  1:174727/1‑251 (MQ=255)                                                                                           aaTAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGCGGTGTCCTTTaccg                                                                                                                                                        >  1:65943/1‑248 (MQ=255)                                                                                           aaTAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAAcg                                                                                                                                                        >  1:156472/1‑251 (MQ=255)                                                                                                aaaaaGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCCGCTACTTTTGTTGCTCCCTGCTCATACTTGACACCTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTcc                                                                                                                                                   >  1:160052/1‑251 (MQ=255)                                                                                                  aaaGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTg                                                                                                                                                 <  1:169004/251‑1 (MQ=255)                                                                                                                   cacaGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCt                                                                                                                                 <  1:34327/250‑1 (MQ=255)                                                                                                                            cccGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGaa                                                                                                                       <  1:199887/251‑1 (MQ=255)                                                                                                                                    gTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTcc                                                                                                                                                                                                                   <  1:147899/151‑1 (MQ=255)                                                                                                                                    gTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTcc                                                                                                                                                                                                                   >  2:147899/1‑151 (MQ=255)                                                                                                                                    gTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTcc                                                                                                               <  1:234848/251‑1 (MQ=255)                                                                                                                                          cagcaTCCCAGCTAATTTAGTTGCACCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGCACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTACCTCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCCTCCAGAAGCCTTTCCCTGaa                                                                                                          >  2:167146/1‑250 (MQ=255)                                                                                                                                              aTTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACAcc                                                                                                     >  2:105259/1‑251 (MQ=255)                                                                                                                                                tCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCCTCCTGAACTATTGCTTCCTGCTCACACCACCCTGCTGTGATACTTCCTCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGCCCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAACCACCAt                                                                                                   >  1:141331/1‑251 (MQ=255)                                                                                                                                                       aCTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACTCCATCCTGgt                                                                                             <  1:188298/250‑1 (MQ=255)                                                                                                                                                                                         ttCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCa                                                           <  1:39699/250‑1 (MQ=255)                                                                                                                                                                                         ttCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCATGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGCTGTTCAc                                                          >  1:235600/1‑251 (MQ=255)                                                                                                                                                                                                        ggCCAATAGTTCATCATGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGGGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAAGCGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTCTCCTGCAGAAGTGACATCATCCTGATGTTcccctcgtattctgacac                                           >  2:178961/1‑234 (MQ=255)                                                                                                                                                                                                           cAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCt                                        >  2:241310/1‑251 (MQ=255)                                                                                                                                                                                                            aaTCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGCAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTg                                       >  1:40581/1‑251 (MQ=255)                                                                                                                                                                                                                   cATCCTGAACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACTCGTCCTGCGTCATCCGCTTCGCTTCATCCAGAAGCCTTTCCCGGAAACACCATCCTGGTGTGTCCGTCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTcgacg                                 <  2:140250/250‑1 (MQ=255)                                                                                                                                                                                                                      ccTGTACTATTGCTTCCTGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCGTTCCCTGAAACACCATCCTGGTGGGTCCTGCAGAAGTGTCATCTTCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAg                              <  2:139736/250‑1 (MQ=255)                                                                                                                                                                                                                                tGCTTCCTGCTCACACCCCCCTGATGTGATACTTCACCCTGAAGTGTCCCTGGCCTTCCTGAACCACCGAATCATGCTGACCGGTTCTCATTCGCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTAACTTCGTTGTCTGACTCCTTGTCGACGAAGATAGAATCgc                    <  2:72635/250‑2 (MQ=255)                                                                                                                                                                                                                                 gCTTCCGGCTCACACCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGAGAGAATGGTCt                  >  2:151335/1‑251 (MQ=255)                                                                                                                                                                                                                                           cacaCCACCCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGa         >  1:123526/1‑250 (MQ=255)                                                                                                                                                                                                                                                 accCTGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTa  <  1:242060/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       CGCTTCAAGCGCCTGCAACTGGAGACGAACGCGGTCATGAGTGGTCATAAAGCAACCTCAATAAGAAAAACTGCCGCGAAGGATAGCAGGAATAAAAAAAGGGAGCACTGTATTCACAGCGCTCCCGGTTCGTTTCGCAGCATTCCAGCTACTTTTGTTGCTCCCTGCTCATCCTTGACAACTTTTCCTCTGGCCTTGCGGCCAATCGTTCATCCTGAACTATTGCTTCCTGCTCACACCACCCCGATGTGATACTTCATCCTGAAGTGTCCCTGGCCTTCCTGACCCACCGAATCATCCTGACCGGTTCTCATTCTCCATCCTGGAGGTGTCCTTTAACGCGTCCTGCGTCATCCTCTTCGCTTCATCCAGAAGCCTTTCCCTGAAACACCATCCTGGTGTGTCCTGCAGAAGTGTCATCATCCTGATGTTCACTTCGTTGTCTGACTCCCTGTCGACGAAGATAGAATCGTCTTTTTCGCGAAGTCTTA  >  NC_000913/2924066‑2924556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑