Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,838,241	G→A	intergenic (‑136/+13)	hydN ← / ← ascG	putative electron transport protein HydN/DNA‑binding transcriptional repressor AscG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,838,241	0	G	A	100.0%	33.5 / NA	12	intergenic (‑136/+13)	hydN/ascG	putative electron transport protein HydN/DNA‑binding transcriptional repressor AscG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/5);  total (7/5)

ATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAAT‑CAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTG  >  NC_000913/2838006‑2838460                                                                                                                                                                                                                                            |                                                                                                                                                                                                                             aTGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTa                                                                                                                                                                                                               <  1:9600/251‑1 (MQ=255)                tttCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATGCATTTACTCGCGTCAGCAAGGATGAACCGGGTCATCAAATTCTCCAGCAATGACAGTTAATGCTCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTTTAATTCAGGAAAAGCGATGGCTTCGACTCTAGAGACTATTAAATACGACGACAATGTTTATCGCGAAgttgcaa                                                                                                                                                                                                 <  2:47117/250‑7 (MQ=255)                                                                                    atgatgAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGc                                                                                                                             >  1:232292/1‑250 (MQ=255)                                                                                                                      aCAGTTCATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATaa                                                                                                                         >  2:112889/1‑220 (MQ=255)                                                                                                                      aCAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATaa                                                                                                                         <  1:112889/220‑1 (MQ=255)                                                                                                                                   cGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAAT‑CAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCggg                                                                            >  1:137030/1‑250 (MQ=255)                                                                                                                                    gATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAAT‑CAGCCGTCCAATAATTTCCTGAATCATCGCAGTCACCGGaa                                                                           <  2:22414/251‑1 (MQ=255)                                                                                                                                                                  tttACTGTTATTTAAGGAACTTAATTTCTGTAATGAAGGAAAATCGGTGTCATAGACACTAGTGACGATGACATATGCTGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAATTTTCAGCTGAAGGTTTTCGGCGGTGAGGCATACCCGCCCTCGAGAATAAAAATACA‑CCGTCCCATAATTTTCTGAATAATCTACGTCACCGGAATTTTTTCCCTGgagcttgccgggacgtggg                                             >  2:36624/1‑235 (MQ=255)                                                                                                                                                                                               gTAATGCAGGAAAAACGAGGTCATCGACACTAGGGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCTCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCTAGCATAAAAAT‑CAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATGTTTACTCTTGAGAGCGCCGGAACGTGGTAGGTGGCAATAGCGATAGCATCGAATCCg                <  2:267561/251‑1 (MQ=255)                                                                                                                                                                                                     cAGGAAAAACGATGTCATCGACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAAT‑CAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGGGTAGGGGGCAATAGCGATATCATCGAATCCGATAACt          >  1:255572/1‑251 (MQ=255)                                                                                                                                                                                                        gAAAAACGATGTCATCGACACTAGTGACGATGACCTATGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGGGAGAAATCCCCGCCATCGAGCATAAAAAA‑CAGCCGTCCAATAATTTTCTGAATCAACTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGGGTAGGGGGCAATAACGATATCATCGAATCCGATAACTGac       >  2:229901/1‑251 (MQ=255)                                                                                                                                                                                                              cgctGTCATCTACACTAGTGACGATGACATATGATGACAATGTTTATCGCGAAGGAGCACTGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGCGAAAAATCCCCGCCCTCGAGCATAAAAAT‑CAGCCGGCCAATAATTTCCTGCATCCTCTCAGTCACCGGAATTTTTACGCTTGAGAGCGCCTGAACGGTGTCGGGGGACATAACGAACTCCTCGAATCCGATAACTGACAcccg  >  2:131004/4‑248 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                             ATGCACAGTCCTGATTTTCCTGATGAGAAACCACGCACGCTACTTCACAGGTACGGCAACCAATACATTTACTCGCGTCAGCAATGATGAAACGGTTCATCAAATTCTCCAGCAATGACAGTTAATGCGCCGATACATTCACAAATCATGCCAGTTTTTAATTTACTGTTATTTAAGGAAATTAATTTCTGTAATGCAGGAAAAACGATGTCATCGACACTAGTGACGATGACATGTGATGACAATGTTTATCGCGAAGGAGCAATGAGTGAGTCGCGGCGGATCAGTTTTCCGCTGAAGGTTTTCGGCGGTGAGAAATCCCCGCCATCGAGCATAAAAAT‑CAGCCGTCCAATAATTTCCTGAATCATCTCAGTCACCGGAATTTTTACGCTGGAGAGCGCCGGAACGGTGTAGGGGGCAATAGCGATATCATCGAATCCGATAACTGACACCTG  >  NC_000913/2838006‑2838460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑