Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,613,763	G→A	E622K (GAA→AAA)	hyfR →	DNA‑binding transcriptional activator HyfR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,613,763	0	G	A	100.0%	89.0 / NA	28	E622K (GAA→AAA)	hyfR	DNA‑binding transcriptional activator HyfR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/16);  total (12/16)

TGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAGAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGACTTGCAGTTGAGCGCC  >  NC_000913/2613518‑2613972                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                  tGAATAGCGCAATTGACGCCATCCCGACCGCGGCACTACGCCAGTTGATGTCGTGGGCTTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGACGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACgaaaaaga                                                                                                                                                                                                               >  2:127029/1‑250 (MQ=255)  gAATCGCGCCATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAAc                                                                                                                                                                                                             >  1:59643/1‑251 (MQ=255)                          ggccgaGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACCTGCGCGAGCTGGAAAACGTGATGGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAATTGTCCGACAAAGCCGTTTACTGCCGACTCTAAATGAAGATTCAGCGCTTCTCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGTCGAAAAAGAACGTCAGCGCATTGGTCAGGTATTGc                                                                                                                                                                                     <  2:170299/249‑1 (MQ=255)                            ccgaGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCg                                                                                                                                                                                    >  1:87289/1‑250 (MQ=255)                                      aCGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATGTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCaa                                                                                                                                                                           <  1:179386/249‑1 (MQ=255)                                                                         tGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACCTCTAAATATCCGACAAAGCCGTTTACTGCCGACGATAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGCGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGAcgcgt                                                                                                                                      >  1:253322/1‑250 (MQ=255)                                                                                        aCGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAgcgc                                                                                                                       >  2:52263/1‑251 (MQ=255)                                                                                                gAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGGATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACgct                                                                                                               <  1:162965/251‑1 (MQ=255)                                                                                                              gTTGACTCGTGGTAACAGTCTGACTTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCCCGAATGc                                                                                                 >  2:63271/1‑251 (MQ=255)                                                                                                                     cGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCgg                                                                                            <  2:91827/249‑1 (MQ=255)                                                                                                                              aGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGAAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGGTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGAtctc                                                                                 <  2:207050/251‑1 (MQ=255)                                                                                                                                         aCATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGAGTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCTGTTCGCGAg                                                                       <  2:238590/250‑1 (MQ=255)                                                                                                                                               caaTGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGATTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGCTTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGtgttgt                                                                >  2:257529/2‑251 (MQ=255)                                                                                                                                                 aTGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTa                                                               >  1:244724/1‑250 (MQ=255)                                                                                                                                                       gACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGAGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGTTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGATGTGTTGTAATCTGCt                                                        <  2:202009/251‑1 (MQ=255)                                                                                                                                                       gACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTACAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCt                                                        <  2:59643/251‑1 (MQ=255)                                                                                                                                                         cAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCACGAGAATGACGAAAAAGAACGTCAGCGCATTGATCAGGAATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAAGGCAGCGGCTGGGGCTCTCGGGTCGCGAGGTGTTGTAATCTGCttt                                                      >  1:165786/1‑251 (MQ=255)                                                                                                                                                          aaaGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCtttt                                                     <  2:229364/251‑1 (MQ=255)                                                                                                                                                           aaGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTGGCCGGGCCCCGTGGCGCAGCGACGCGATGAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTg                                                    <  1:248314/251‑1 (MQ=255)                                                                                                                                                                       gCCGACTCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTTTAATCTGCTTTTGCAGGAGTATg                                          <  1:74239/249‑1 (MQ=255)                                                                                                                                                                        ccGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCGCGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCAt                                       <  2:249561/251‑1 (MQ=255)                                                                                                                                                                         cGACGCTAAAGGAAGATGCAGCGCTGCGCAGTGCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAATAACGTCAGCGCAGTGTTCAGGTATAGCGAAAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGTATGAAGCGCACCACGCGGCTTTCACGAATGCAGCGGCTGGGGATCGCGGGTCGCGAGGTGTTGTAATTTGCGTGTGCAGGAGTATGCAtt                                      <  2:129089/251‑2 (MQ=255)                                                                                                                                                                                    gAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGACAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGCGAAACAAAct                           >  1:181212/1‑251 (MQ=255)                                                                                                                                                                                     aaGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGAAGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAActc                          >  2:268375/1‑251 (MQ=255)                                                                                                                                                                                         ttCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGACCGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCtt                      >  1:245413/1‑251 (MQ=255)                                                                                                                                                                                           cAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATGGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTc                    <  1:257529/251‑1 (MQ=255)                                                                                                                                                                                               gcTTCGCAGTTCACTGGCGCAGTTACTGCCCCTGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGAGCAGCGACGCGCTTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGGGAAACAAACTCTCTTTCGACt                >  2:21556/1‑251 (MQ=255)                                                                                                                                                                                               gcTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAAAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCGCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGACt                <  2:87289/251‑1 (MQ=255)                                                                                                                                                                                                              ggCGCAGTTACTGCACCCGAAGACGCCAGAGAATGACGAAAAAGAACGTCAGAGCATTGTTCAGGGATTGCGAGAAACCAATGTCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGGGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGACTTGCAGTTGTGCGcc  <  2:217528/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                  TGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCGGGCGGTACTGTTGACTCGTGGTAACAGTCTGAATTTACATCTAAATGTCCGACAAAGCCGTTTACTGCCGACGCTAAATGAAGATTCAGCGCTTCGCAGTTCAATGGCGCAGTTACTGCACCCGACGACGCCAGAGAATGACGAAGAAGAACGTCAGCGCATTGTTCAGGTATTGCGAGAAACCAATGGCATTGTTGCCGGGCCCCGTGGCGCAGCGACGCGATTAGGGATGAAGCGCACCACGCTGCTGTCACGAATGCAGCGGCTGGGGATCTCGGTTCGCGAGGTGTTGTAATCTGCTTTTGCAGGAGTATGCATGAGAAACAAACTCTCTTTCGACTTGCAGTTGAGCGCC  >  NC_000913/2613518‑2613972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑