Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,582,272	C→T	G169E (GGG→GAG)	ypfG ←	DUF1176 domain‑containing protein YpfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,582,272	0	C	T	96.0%	60.7 / NA	25	G169E (GGG→GAG)	ypfG	DUF1176 domain‑containing protein YpfG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (10/14);  minor base G (0/1);  total (10/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

AAATCAATGGTGTTATAGGCCCCTGCCTCACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCCCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCCACTCAGAGAAATGGTCTGATCGCCATCGCGTAAGGTGATTGCCTTCCCTTCCTGAATCATCTGCAAAAACGCGGTGATGGTTGCCGTATCATCGGTTACTAATAACCATGGTGAAATCCGCCACTTGTCACCACTTAACGCCAACGGCTC  >  NC_000913/2582026‑2582498                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   aaaTCAATGGTTTTATAGGCCCCTGCCTCACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCTCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCttt                                                                                                                                                                                                                                <  2:47053/251‑1 (MQ=255)        tGGTGTTATAGGCCCCTGCCTCACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACAACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCTTCCTCTTTCTTGATc                                                                                                                                                                                                                         >  1:243497/1‑251 (MQ=255)                 aGGCCCCTGCCTCACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCTACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCTTCTTGCTTGTTCCACGCGGtt                                                                                                                                                                                                                <  2:19068/251‑1 (MQ=255)                    ccccTGCCTCACATCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCTCCTCACGACGCAAGGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCCTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCCACGCGGTTTCa                                                                                                                                                                                                             <  2:115696/251‑1 (MQ=255)                                    aaTCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCtttt                                                                                                                                                                                             <  2:85804/251‑1 (MQ=255)                                    aaTCACCATCAGCGCTTTAGCATCAGGCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGGGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCCACGCGGTTTCACTGCCAACgcgcgtt                                                                                                                                                                                              <  2:148828/250‑4 (MQ=255)                                                      atcatcAGTCAGCGCAGTGACATTCTCCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCGCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAaac                                                                                                                                                                            <  2:203983/250‑1 (MQ=255)                                                                                                                     aTTCATCCGCCCGTTTCCACAATCCAGAAAATCGTTGCGTTCTTACACTGAGCGTGGCGTCTGCGTTGGCTTAACCACCGCGACCTCTTTCCGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGCTCCTTCTCTTTCTTGATCCACCCGGTTTCCCTGCCAACGCGCTTTTGATGTGCATCAATAAACAACAACGCTGATTTTAAGCCACTCAGAGACATGGTTTGATCACCACAGCGTAAGGTGATTGcct                                                                                                            >  2:19776/1‑251 (MQ=255)                                                                                                                           ccGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCCACTCAGAGAAATGGTCTGATCGCCATCGCGTAAGGTGATTGccttccctt                                                                                                      <  1:149463/251‑1 (MQ=255)                                                                                                                             gCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCCACTCAGAGAAATGGTCTGATCGCCATCGCGTAAGGTGATTGCcttcccttcc                                                                                                    <  1:73741/251‑1 (MQ=255)                                                                                                                                aGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCCACTCAGAGAAATGGTCTGATCGCCATCGCGTAAGGTGATTGCCTTCCCTTCCTGa                                                                                                 <  1:215315/251‑1 (MQ=255)                                                                                                                                   ttCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCCACGCGGTTTCACTGCCCACGCGCTTTTGCTGAGCATCAATTAACAACAACGCTGCTTTTAAGCCACTCAGAGAAATGGTCTGTTCGCCATCACGTAAGGTGATTGCCTTCCCTTCCTGAatc                                                                                              >  1:3337/1‑251 (MQ=255)                                                                                                                                      cATAATCCAGCAAATCGATGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGATCGTCCTCTTTCTTGGTCCACGCGGTTTCCCTGCCAACACCCTTTTGCTGAGCCTTCATAAACAACAACCCTGCTTTTATGCCACTCCGAGAAATGGTCTGAGCGCCATCTCGTAAGGTGAATGCCATCCCTTCCTGAatcatc                                                                                           >  2:208513/1‑251 (MQ=255)                                                                                                                                                 aaaTCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCCCCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCCTCCTCTTTCTTCACCCACCCGGCTTCCCTGCCAACGCGATTTTGCTTGGCATCAATAAACACCAACGCTGCTTTTAACCCACTAAGAGGAATGGTCTGTTCGGCATCGCGTAAAGTGAATGCCTTCCCTTCCTGAATCATCCGCaaaaatgc                                                                                >  1:254013/1‑248 (MQ=255)                                                                                                                                                    tCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCCCCGCGGGTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCCACTCAGAGAAATGGTCTGATCGCCATCGCGTAAGGTGATTGCCTTCCCTTCCTGAATCATCCGCAAAAACGCggg                                                                             >  2:205138/1‑250 (MQ=255)                                                                                                                                                     cGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCCACTCAGAGAAATGGTCTGATCGCCATCGCGTAAGGTGATTGCCTTCCCTTCCTGAATCATCTGCAAAAACGCGGTg                                                                            <  1:169202/251‑1 (MQ=255)                                                                                                                                                                            ggCGTCGGCGTTGGGTGGACCACCGCGACCTCTTTCAGCGCCGGCGCGGGCTGTTCGTTGGGCTGCGGTTCGTCCTCTTTCATGATCCACGCGGGTTCACTGCCAACGCGCTATTTCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCCACTCAGAGATATGGTCTGATGGCCATCGCGTAATGTGATTGCCTTGCCTTCCTGAAACAGCTGCAAAAGCGCtgtgttgtccgccgtgttttcggttac                                                     <  2:243602/251‑24 (MQ=255)                                                                                                                                                                                    cGTTGGGTTAACCACCGCGACCTCGTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCCACTCAGAGAAATGGTCTGATCGCCATCGCGTAAGGTGATTGCCTTCCCTTCCTGAATCATCTGCAAAAACGCGGTGATGGTTGCCGTATCATCGGTTACTAATAAcc                                             <  1:64351/251‑1 (MQ=255)                                                                                                                                                                                      ttgggctcacccccGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCAACGCGGTTTCCCTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCAACTCAGAGAAATGGTCTGATCGCCATCGCGGAAGGGGGTTGCCTTCCCTTCCTGAATCGTCTGCAAAAACGCGGGGATGGGTGCAGTAACATCGGTTACTAAAAACCa                                            >  2:176107/13‑250 (MQ=255)                                                                                                                                                                                             aaCCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCCACTCAGAGAAATGGTCTGATCGCCATCGCGTAAGGTGATTGCCTTCCCTTCCTGAATCATCTGCAAAAACGCGGTGATGGTTGCCGTATCATCGGTTACTAATAACCATGGTGaaa                                    <  2:198489/251‑1 (MQ=255)                                                                                                                                                                                             aaCCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCCACTCAGAGAAATGGTCTGATCGCCATCGCGTAAGGGGATTGCCTTCCCTTCCTGAATCATCTGCAAAAACGCGGTGATGGTTGCCGTATCATCGGTTACTAATAACCATGGTGaa                                     >  1:31138/1‑250 (MQ=255)                                                                                                                                                                                                 accGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTTATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCCTCAATAACCAACAACGCTGCTTTTAAGGCACTCAGAAAAATGGTCTGATCGCCATCGCGGAAGGAGATTGACTTCCCTTCCTACATTATATGCAAAAACGCGGTGATGGTTGCCGTATCATCGGTTACTAATAACCATGGTGAAATCCg                                >  1:265071/1‑251 (MQ=255)                                                                                                                                                                                                   cgcgACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCCACTCAGAGAAATGGTCTGATCGCCATCGCGTAAGGTGATTGCCTTCCCTTCCTGAATCATCTGCAAAAACGCGGTGATGGTTGCCGTATCATCGGTTACTAATAACCATGGTGAAATCCGcc                              <  1:247053/251‑1 (MQ=255)                                                                                                                                                                                                                          cgggcggTACGCTGAGCGGCGGTTCGTCCTCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCCACTCAGAGAAATGGTCTGATCGCCATCGCGTAAGGTGATTGCCTTCCCTTCCTGAATCATCTGCAAAAACGCGGTGATGGTTGCCGTATCACCGGTTACTAATACCCATGGGGAAATCCGCCTCTTGTCACCACTTAACGCCAAc       <  2:82402/251‑1 (MQ=255)                                                                                                                                                                                                                               ggTACGCTGAGCGGCGGTTCGTCCTCTTTTTTGATCCACGCGGTTTCACTGCCCACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCCACCCAGAGAAATGGTCTGATCGCCATCGCGGAAGGTGATTGCCTTCCCTTCCTGAATCATCTGCAAAAACGCGGTGATGGTTGCCGTATCATCGGTTACTAATAACCATGGAGAAATCCGCCCCTTGTCACCACTTAACGCCAACGGccc  >  2:87527/1‑249 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   AAATCAATGGTGTTATAGGCCCCTGCCTCACAGCTAATCATCATCAGCGCTTTATCATCAGTCAGCGCAGTGACATTCACCTCACGACGCAATGGATCAAGCGAGCAGCGCAGACCATTCATCCGCCAGTTTCCATAATCCAGCAAATCGTTGCGTTCTTCGAGTGAGAGTGGCGTCGGCGTTGGGTTAACCACCGCGACCTCTTTCAGCGCAGGCGCGGGCGGTACGCTGAGCGGCGGTTCGTCCCCTTTCTTGATCCACGCGGTTTCACTGCCAACGCGCTTTTGCTGAGCATCAATAAACAACAACGCTGCTTTTAAGCCACTCAGAGAAATGGTCTGATCGCCATCGCGTAAGGTGATTGCCTTCCCTTCCTGAATCATCTGCAAAAACGCGGTGATGGTTGCCGTATCATCGGTTACTAATAACCATGGTGAAATCCGCCACTTGTCACCACTTAACGCCAACGGCTC  >  NC_000913/2582026‑2582498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑