Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,577,937	T→C	E147E (GAA→GAG)	maeB ←	malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,577,937	0	T	C	100.0%	53.3 / NA	17	E147E (GAA→GAG)	maeB	malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

GCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCT  >  NC_000913/2577694‑2578141                                                                                                                                                                                                                                                    |                                                                                                                                                                                                             gCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATCCCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAatat                                                                                                                                                                                                       >  1:116513/1‑251 (MQ=255)         agataACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACa                                                                                                                                                                                                <  1:226621/250‑1 (MQ=255)           ataACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTc                                                                                                                                                                                             >  1:157704/1‑251 (MQ=255)             aaCGCCTTTGGAACCGCAAACCACTATGTTATGTTTGTGAAGCCCCAGCCCTACCAGCAGTTTCATACATGCGATTGCGGCGGCAACCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTGTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTTCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTg                                                                                                                                                                                           <  2:259405/251‑1 (MQ=255)                                aCCACGATGTTATGTTGTTGCAGGCCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCCCGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGTAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAg                                                                                                                                                                        <  2:108221/251‑1 (MQ=255)                                        gttatgttTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTACTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATgc                                                                                                                                                                <  2:195360/251‑1 (MQ=255)                                          tatgttTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATgcc                                                                                                                                                               <  2:70122/250‑1 (MQ=255)                                                                                cAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATaaa                                                                                                                        <  1:194783/251‑1 (MQ=255)                                                                                                        ccGGAAACCGCCATCCGCACGTCGGGGATGTTTTGCTCCCCCACGCGCAAGCCTTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATGCCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATAAAGAAACATTCTGGCGCGTTAATGTCTTCGAGTTTGATGCCGCCGAAGGTTGGGTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGGCGAGTTCGTCAAc                                                                                                <  2:83812/251‑1 (MQ=255)                                                                                                               ccaccaTACACACGCCGAAGAAGTTTTTCTCCAACACATTCAAGCCGTTGAGGATGGAGGCAGTGCTGATACTTGCCGTGCCGTGCTGAACGTAGTGGAATACAGGAATATTCATACGCTCGCCCCATTTCTGCTCACTATAGAAACATTATGGCGCTTTACTGTCTTCGCGGATGATGCCGCCGAAGGATGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGTGTCGAGTTCGTAAACTTCAATg                                                                                         >  2:245418/1‑251 (MQ=255)                                                                                                                                             ccaccaCGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCTTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCGCAATATAGAAACATTCTGGCGCTGTAATGTCTTCGAGGTTGATGCCTCCGAAGGGTGGTTCGAGCGCGGCGACAACTGCAATAAAGGTGTCCGGGTCGAGTTCGTCAACGTCAATGTCAAATACATCAAGCCCGTCGAATTACtt                                                            <  2:44688/250‑1 (MQ=255)                                                                                                                                                               tGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTATGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCt                                         >  2:117015/1‑251 (MQ=255)                                                                                                                                                                gAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCt                                         <  1:112609/250‑1 (MQ=255)                                                                                                                                                                     tGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATc                                   >  2:50556/1‑251 (MQ=255)                                                                                                                                                                            aGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGtt                            >  2:95698/1‑251 (MQ=255)                                                                                                                                                                                   aTAATTGCCGTGCCGAGATAATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCACCTTCAATGTCACATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTtgcctgc                     >  2:223369/1‑251 (MQ=255)                                                                                                                                                                                                       aTCGTCGTGGATTACAGGCATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTAGAGCGAGGCGCCAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCACATACATCAATCCCGGCGAATTGCTTAAACAGAACGCACTTGCCTTCCATCACAGGCTTGACTGCCAGCGCGCCAATGTTGCCt  >  2:29232/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                             GCCCTGATAGATAACGCCTTTTGAATCGCAAACCACGATGTTATGTTTTTGCAGACCCAGCGCTACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGCCTTCCATCACCGGTTTGCCTGCCAGCGCGCCAATGTTGCCT  >  NC_000913/2577694‑2578141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑