Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,410,419	G→A	L312L (CTG→TTG)	yfbS ←	putative transporter YfbS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,410,419	0	G	A	95.5%	66.5 / NA	22	L312L (CTG→TTG)	yfbS	putative transporter YfbS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (7/14);  minor base C (1/0);  total (8/14)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAGTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTC  >  NC_000913/2410178‑2410661                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   aGCATACCTATCAGTTTCCAGTTACCCACAACCAGGAGGATATCGCCCAGCAGTAGAGGCTCATCCGCCTGCGAACCTCCCAGCCCCACGCCATTGCGCTTTAGCCCCTCCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCGCCGATTTACCAATCAGTGCTGACTCAGGAATTAATAAAATCGCGGGCATGCCCACATCAAGCGCCTGGTCGTAAAAATGCTCGCCGCGCAATACAATCgg                                                                                                                                                                                                                                           <  2:119928/251‑1 (MQ=255)                        aCCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCGCACTACaa                                                                                                                                                                                                                     <  2:156736/250‑1 (MQ=255)                                     atgatATCGCCCAGCAGCAGAGTCTCTTCCGCCAGCGAACCGTCCAGCGCCGCGCCAATGCGCTTTAGCCCCACCACATTCAGTCCGTATCGGGTACTAAAGCCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACAACAAGGGCGTGGTCAGAAAAATACTCGCCGCGCAATACCGTCAGCTCCAGCAATTGGTCTCTACAATATTGCCGGAGATc                                                                                                                                                                                                       <  2:138423/251‑1 (MQ=255)                                       gatACCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCGCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGTCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCg                                                                                                                                                                                                      <  2:93138/250‑1 (MQ=255)                                                              cctCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCTCCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCTGAGATCGACATCAGCCGCAGACATATCAAtc                                                                                                                                                                              <  2:93299/249‑2 (MQ=255)                                                              cATCCGCCAGCGAACCGTCCAGCGCCACGCCATTGCGCGTCAGCCCCACCTCATGCAGTCCGTAGCGGGTACGAAAACCAATTTCGCACACCGATTTACCAATCAGTTCTGACTCAGGAATTATAGAAATCCCTGCCATGCCCACATCAAGGGCCTGGCCAGAAAACTACTCGCCGCGCAATACCATCGGCTCCAGCAAGTGCTAACTACAAAATTGCCGGCGATCGTCATCAGCCgtagtaatttcaa                                                                                                                                                                                <  2:150938/249‑13 (MQ=255)                                                                 ccGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGc                                                                                                                                                                           <  1:31768/251‑1 (MQ=255)                                                                   gCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGCCATCAGCCGCAGACATATCAATAAGCa                                                                                                                                                                          >  2:196221/1‑250 (MQ=255)                                                                     cAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCaaa                                                                                                                                                                        >  2:99590/1‑250 (MQ=255)                                                                      aGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAAc                                                                                                                                                                      <  2:146860/251‑1 (MQ=255)                                                                          aaCCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACAGCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCa                                                                                                                                                                  <  1:10881/251‑1 (MQ=255)                                                                                        acgccaTTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTc                                                                                                                                                    >  2:103102/1‑251 (MQ=255)                                                                                              ttGCGCTTTAGCCCAACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCACTTGCTCACTACAAAATTGCAGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAAc                                                                                                                                              >  1:71240/1‑251 (MQ=255)                                                                                                                                                      cACCGATTTACCAATCAGTTCTGACTTAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAAGTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACTCGCGCGAAATTCAGAAACCCCTTTAACGTTCACGATAACGCTACGAAAACGCCGCCAGCGTTCAACACCGATGa                                                                                      <  1:235910/251‑1 (MQ=255)                                                                                                                                                        ccGATTTACCAAACAGGTCTGACTCAGGAATAAATGAAATCGCTGCCATGCCCACATCAAGGGCCTGGGCAGAAAAATCCTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCATCCGCAGACATATCAATAAGCAAACCGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACTCCGATGACg                                                                                    <  2:175437/251‑1 (MQ=255)                                                                                                                                                               aCCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAgcgc                                                                             >  1:188287/1‑251 (MQ=255)                                                                                                                                                                             aCTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGt                                                               <  2:31028/251‑1 (MQ=255)                                                                                                                                                                                        aaTGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGatc                                                    <  1:80551/251‑1 (MQ=255)                                                                                                                                                                                              aTCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCACTACCATCGGCTCCAGCAATTGCTCACTACAAAATAGCCGGAGATCGAAATCAGCCGCAGACAGATCAATAAGAAAAACGTCACGCGCACGAACTTCAGAAACCACATTAACATTGACGATAACGCGACGAACACGACGCCAGCGTTCAACACAGATGAAGTTAGCACCATAACACTCACGTAATTTTAGatcatca                                                >  1:259564/1‑248 (MQ=255)                                                                                                                                                                                                          cccACATCAAGGGCCTGGACAGCAAAATCCTCGCCGCGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATTACTAAGCAACACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGAGCCATAACGCTCACGTCATTTGAGATCATCCAGCCGTTGACCAAt                                  >  2:130466/1‑251 (MQ=255)                                                                                                                                                                                                                                          gccgcGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGCGATCGACATCAGCCGCAGACATATCCATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTc  >  1:17874/1‑251 (MQ=255)                                                                                                                                                                                                                                          gccgcGCAATACCATCGGCTCCAGCAATTGCTCACTACAAAATGGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCATACGTc  <  2:40703/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   AGCATACCGATCAGTTTCCAGTTACCCACAACCAGGATGATATCGCCCAGCAGCAGAGGCTCATCCGCCAGCGAACCTTCCAGCGCCACGCCATTGCGCTTTAGCCCCACCACATTCAGTCCGTAGCGGGTACGAAAACCAATTTCGCGCACCGATTTACCAATCAGTTCTGACTCAGGAATTAATGAAATCTCTGCCATGCCCACATCAAGGGCCTGGTCAGAAAAATACTCGCCGCGCAGTACCATCGGCTCCAGCAATTGCTCACTACAAAATTGCCGGAGATCGACATCAGCCGCAGACATATCAATAAGCAAAACGTCACGCGCGCGAAATTCAGAAACCCCATTAACGTTCACGATAACGCGACGAAAACGCCGCCAGCGTTCAACACCGATGACGTTAGCGCCATAACGCTCACGTAATTTGAGATCATCCAGCCGTTGACCAATCATTGGCGATCCGGGGCGAATAGCCAGACGTC  >  NC_000913/2410178‑2410661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑