Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,384,808	C→T	K305K (AAG→AAA)	yfbK ←	IPR002035/DUF3520 domain‑containing protein YfbK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,384,808	0	C	T	100.0%	39.5 / NA	14	K305K (AAG→AAA)	yfbK	IPR002035/DUF3520 domain‑containing protein YfbK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/5);  total (9/5)

TACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCA‑TTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATA  >  NC_000913/2384568‑2385018                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                   tACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCA‑TTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGCTGCCGCCCTTAATAAACCCTTTCGTCGCct                                                                                                                                                                                                          >  1:185060/1‑251 (MQ=255)            cTTCAGAGAGGGTATCAATGTAGCTGTAGCTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAATAACACCAGACTCCCGCTGTTTTTTGACCA‑TTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTAACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGcc                                                                                                                                                                                               >  1:60907/1‑251 (MQ=255)                                 aGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCA‑TTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGcc                                                                                                                                                                           >  1:184423/1‑250 (MQ=255)                                                    aaCATCGGAAATTCGCCCCATCATTGCCTCGTTTGAATTGCTATTCCCCACGCCAAACGCCGACAGATTAACACCAGACTCCCGCTGTTTTTTGACCA‑TTGATTCAATCGATTTTGGATCGTCAATGCAAACGTAAAAGTCACCGTCAGTGGCTAATAAAAGGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCAt                                                                                                                                                       <  2:3854/251‑1 (MQ=255)                                                             aaTTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCA‑TTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAAt                                                                                                                                              >  1:59685/1‑251 (MQ=255)                                                                                              aTTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCA‑TGAATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGAGGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTcc                                                                                                             <  1:132678/251‑1 (MQ=255)                                                                                                   ccccGCCAAACGCCGCCAGGTTAACCCCAGACCCCCGCTGTTTTTTG‑CCACTGGATTCAATCGATTGTGTAGCTTCAATGCAAACTTTAAAGCCACCGTCAGTTGCTAATAAAATGCAATTGATTCCCCCCTTAATAACCCCTTTCTCCTCCTGCTTATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTTCCTTCGGCATCCAGCGAATCAATTGCGGTATTAATTTCCGCCTTATGACTCCCTgag                                                                                                        <  2:220301/248‑1 (MQ=255)                                                                                                                                       gCTGTTTTGAGACCA‑ATGATTCCATCGATTTTGGAGCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGATAATAACATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGACAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGt                                                                      >  2:118320/1‑249 (MQ=255)                                                                                                                                                             cAATCGATTTTGGATCGTCAATGCCAACGTTAAATTCACCGTCAGTGGCTAATAAAATGCGATTGAGGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATcc                                               <  1:233501/251‑1 (MQ=255)                                                                                                                                                                                         gTTAAAGTCACCTTCAGTGGCTAATAAAAGGCGATTGATTCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAAtt                   <  1:27407/251‑1 (MQ=255)                                                                                                                                                                                           tAAAGACACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTc                 >  2:243545/1‑251 (MQ=255)                                                                                                                                                                                            aaaGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACACTTTCGTCGCCTGCTGATAAGCCAGTTACAGACCGGCACCGCCCTTGGTACTGCGTTCGGCATCCAGCGAATCAATTGCGGCATTACTTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGAAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTc                 >  1:259536/1‑250 (MQ=255)                                                                                                                                                                                                         gTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCATCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGCGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGAt   >  1:203066/1‑251 (MQ=255)                                                                                                                                                                                                             cTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCTTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGACTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGACGTTCTTTGACCAATAATTTCAACGAAGACTGGATa  >  1:186591/1‑248 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                   TACTTTCTGCGCTTCAGAGAGGGTATCAATGTAGCTGTAGTTGCCGTTACCAACATCGGCAATTCGCACCATCATTGCCTCGTTGTAATTGCTATTCCCCACGCCAAACGTCGACAGAGTAACACCAGACTCCCGCTGTTTTTTGACCA‑TTGATTCAATCGATTTTGGATCGTCAATGCCAACGTTAAAGTCACCGTCAGTGGCTAATAAAATGCGATTGATGCCGCCCTTAATAAACCCCTTCGTCGCCTGCTGATAAGCCAGTTCCAGCCCGGCACCGCCATTGGTACTGCCTTCGGCATCCAGCGAATCAATTGCGGCATTAATTTCCGCCTTATGACTCCCGGAGATAGAAGGCAATGCAATACGGGAGTCGCCAGCGTAGGTCACGATGGCAATGTTATCCTGCTCACGAAGTTCTTTGACCAATAATTTCAACGAAGACTGGATA  >  NC_000913/2384568‑2385018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑