Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,204,395	T→A	intergenic (+106/‑201)	yehK → / → yehL	uncharacterized protein YehK/putative AAA(+) MoxR family ATPase YehL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,204,395	0	T	A	100.0%	48.3 / NA	18	intergenic (+106/‑201)	yehK/yehL	uncharacterized protein YehK/putative AAA(+) MoxR family ATPase YehL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (9/9);  total (9/9)

AATAAATGATGCGTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACCGCAGAATAATCATCTGCAGCGTCCGCCTGCTGCTGTG  >  NC_000913/2204157‑2204640                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      aataaATGATGCGTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTgg                                                                                                                                                                                                                                            >  1:103925/1‑250 (MQ=255)  ataaATGATGCGTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGaa                                                                                                                                                                                                                                          <  2:249700/251‑1 (MQ=255)                           gagaTGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGgtgt                                                                                                                                                                                                                 <  1:65885/251‑1 (MQ=255)                              aTGAGTGTATTGATGAGTGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGAAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGGTGCACAGGCTGTGGGGtt                                                                                                                                                                                                                >  2:185597/1‑249 (MQ=255)                                                                                           tAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGAt                                                                                                                                                  <  1:205023/250‑1 (MQ=255)                                                                                                            atatatTCAACCAAGAAATAGAATAATATACTATAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGCTAtttt                                                                                                                                >  1:98129/1‑251 (MQ=255)                                                                                                                        aaGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAaaca                                                                                                                     >  2:149879/1‑250 (MQ=255)                                                                                                                                     gtataCTATATATAATTAAAATTGGTCCGTTCCGGCGCAACCTTCCAATGGCCTGGATTATAAATTTCATTATCTTAATTGCAACGGGGTCCAGCTGTGGTTCTAAATCGTTCGTTGGAACACGATGTTGAACAGGCTGAGGTGTTAGGCCTGGAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATATGCTTTCCAGATATTTTTCGATCAATCAACTAATGCGCCaa                                                                                                        >  2:43791/2‑250 (MQ=255)                                                                                                                                      tataCTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAAc                                                                                                      >  1:161466/1‑251 (MQ=255)                                                                                                                                              aaaTAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGTGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTTTTATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAATCACTTATTg                                                                                              <  2:256112/251‑1 (MQ=255)                                                                                                                                                               ccGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTGTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGttt                                                                             <  1:165539/251‑1 (MQ=255)                                                                                                                                                                cGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTa                                                                            <  1:4460/251‑1 (MQ=255)                                                                                                                                                                              gTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACAt                                                              >  1:79489/1‑251 (MQ=255)                                                                                                                                                                                cccAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACAtt                                                             <  2:79561/250‑1 (MQ=255)                                                                                                                                                                                                                tAATTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACCGCAGaataat                             <  2:161466/250‑1 (MQ=255)                                                                                                                                                                                                                  aTTGCAACGGGGTCCAGCCGTGGTTATAAATCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACCGCAGAATAatcat                          >  1:9581/1‑251 (MQ=255)                                                                                                                                                                                                                                 aGCCGAGGTTATAAATCGTTCGTTGGAACACGTTGTTGCACGGGCTGTGGTGTTAGGCCTGAAAATAGAAAATAAAAGAACATGCCTCACCAGCAGGTTTTTTTATCTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAAAAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATATCTCCGCAGAATAATCATCTGATGCGTCCGccg           >  2:25661/1‑250 (MQ=255)                                                                                                                                                                                                                                           ataAATCGTTCGTTGGAACACGATGTTGCTCAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTGTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTTCGCGTAAAATATCGTTTATTTCAGTAATACATTTCAGGGATGAATATATGTCGCCGCAGAATAAGCATCTGCATCGGCCGCCTGCGGCtgtg  <  2:167320/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      AATAAATGATGCGTTGATTTATATAAGAGATGAGTGTATTGATGAGCGGAAATTACGAGGTGAATCACCTGAAACTGTAATAGCAATTTTTAATCGTGAACTACAGAATATATTCAACCAAGAAATAGAATAATATACTCTAAATAATTCAAATTGGTCCGATCCGGCGCAACGTCCCAATGGCCTGGATTATAAATCTCATTATCTTAATTGCAACGGGGTCCAGCCGTGGTTATAATTCGTTCGTTGGAACACGATGTTGCACAGGCTGTGGTGTTAGGCCTGAAAATAGTAAATAAAAGAACCTGCCTCACCAGCAGGTTTTTTTATTTACTGTGATCTGCTTTCCAGATATTTTTCGCTCAAACAACTAATGCGCCAAACATTTATTGCGCGTAAAATATCGTTTATTTCATTAATACATTTCAGGGATGAATATATGTCACCGCAGAATAATCATCTGCAGCGTCCGCCTGCTGCTGTG  >  NC_000913/2204157‑2204640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑