Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,448	C→T	intergenic (‑575/+834)	gatD ← / ← gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,448	0	C	T	100.0%	45.1 / NA	16	intergenic (‑575/+834)	gatD/gatB	galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/9);  total (7/9)

TTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGAT  >  NC_000913/2173205‑2173690                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   ttAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCg                                                                                                                                                                                                                                           >  1:17728/1‑251 (MQ=255)    aGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCCCGAAGAAGCCGATGGTGGCAAGATCGACAAACGACAGCCACTGATTAACCGGCACACAAACACAAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCACC‑‑GCCGTATGTACCAGCAGCACCGCCGGATCAAGCCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCgctg                                                                                                                                                                                                                                        >  1:33894/1‑248 (MQ=255)                         gATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCatgatg                                                                                                                                                                                                                   >  2:138659/1‑251 (MQ=255)                           gccACGGCGCCCGCCCCCGCCACGAAGAAGCCGATGGTGGCAAGATCGCAAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGAAAAAAACCAGGCTTGCC‑GATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATggg                                                                                                                                                                                                                 <  2:202163/249‑1 (MQ=255)                                          aTCGCCACGAAGAAGCCGAGGGTGGCAAGATCGCCAAACGGCAGCACCTGAGTACCCGGCACACAAACAGCAATAAAAAGGGTGAGGGGGATAAAAATCAGGCTTGCC‑GATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTgg                                                                                                                                                                                                  <  1:24881/251‑1 (MQ=255)                                                           gATGGTGGCAAGCTCGACCAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTg                                                                                                                                                                                 >  1:217614/1‑251 (MQ=255)                                                                            caacgggCACCACCGGATTCCCCGGCACCAAAACAGCAATTAAAAGGGTGAGTGGGAAAAAAAGCAGG‑TTGCCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCCCCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                                                                                                <  2:91191/246‑1 (MQ=255)                                                                                                                                   gaaaaaacacatgtttGCC‑TATACCACCC‑‑CCGTTGGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGAAACCCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCCCCCGTGGCATTAGCAGCATCACTGCCGCCGTGTTTACCGCCAGCTGCAATACACCTTTGACAGCGTAACCCGCGAGGATGCCGATAATCAGCCCCa                                                                                                         <  2:220860/237‑1 (MQ=255)                                                                                                                                        aaaaaaccgggtttgcagatccccccGCCGTATGTCCCAGCAGCAACGCCGGAACAAGGCCAATCAGGACCCCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATc                                                                                                       <  2:51614/229‑1 (MQ=255)                                                                                                                                                                   ccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCa                                                                            <  1:252500/251‑1 (MQ=255)                                                                                                                                                                     gTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCaaa                                                                          <  1:138659/251‑1 (MQ=255)                                                                                                                                                                             cagcagCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCGCGCCAAATGGACCa                                                                   >  1:196189/1‑250 (MQ=255)                                                                                                                                                                                  gcaccGCCGGAGCAAGGCCAATAAGGAACTCCAGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGTTTTAATCACCCGTGGCATTATCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATTCACCTTTGACATCGTAACCCTCGAGGATGCCGATAATCAGCCCCATCTTAAAACCCACGGTGACAGGCTCGCGAAATGGTCCAAATTTg                                                             <  2:91302/247‑1 (MQ=255)                                                                                                                                                                                                                      cgccgAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGtt                          >  1:105223/1‑250 (MQ=255)                                                                                                                                                                                                                                         aCGACTACGATCTTGCTTAGGGATGGGCGTTAAACCACCCATGATGGGTGTAATCACCCGTGGCATAAGCAGCTACACTGCCGCCGTTTTTTCCGCCAGCTGCAATACACCGTTGACATCGTAACCCGCGAGGATGCCTATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGGTGAATATCGTCGGCGCTAAAATTAATTCGGTTAACGCCTGGGATTTTCGCga      <  2:97387/251‑1 (MQ=255)                                                                                                                                                                                                                                             cTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGTGATTTTCTCgatgat  >  1:95357/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   TTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCC‑GATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGAT  >  NC_000913/2173205‑2173690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑