Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,622	A→T	E274D (GAA→GAT)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,622	0	A	T	97.1%	86.7 / NA	34	E274D (GAA→GAT)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (9/24);  minor base G (0/1);  total (9/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

CGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTG  >  NC_000913/3386‑3857                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            cgccgcCAGGATTGCATTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGt                                                                                                                                                                                                                               <  1:250570/251‑1 (MQ=255)          gATTGCAGTGCGCACGGGCGACATCTGGCTGGCTTCATTCACGCCGGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCCGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTAGCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCACAGCGCGTTGCCGACtt                                                                                                                                                                                                                      <  2:147270/251‑2 (MQ=255)               cattgcGCACGGGCGCCATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGGTgg                                                                                                                                                                                                                 >  2:262801/1‑251 (MQ=255)                            cGACATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCt                                                                                                                                                                                                    <  1:130786/251‑1 (MQ=255)                              aCATCTGGCTGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGTCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCGCCGGCCCGACCTTGTTCGCTCTGTGGGACAAGCCGTAGACCGCCCAGCGCGTGGCCGACTGGTTGGGTAAGAACTTCCTGc                                                                                                                                                                                                  <  2:94956/251‑1 (MQ=255)                                     gctggCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGCAATCGGCGCGGGAGCGAGCGGTATCTCCGGCTCCGGCCCGCCCCTGTTACCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGGCGACTGGTTGGGTAAGAACTACCTGCCAAATc                                                                                                                                                                                            >  2:80635/4‑250 (MQ=255)                                             cattcaCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCCCCGGCCCGACCTTGGTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTTCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAgggt                                                                                                                                                                                   >  1:82946/1‑249 (MQ=255)                                             cattcaCGCCTGCTATTCCCGGCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGATCCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGtt                                                                                                                                                                                   <  1:151885/251‑1 (MQ=255)                                               ttcaCGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGCTGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGCTGCGGAAATCGGCGCGGGAACGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGCGTGACAAGCCGGGTACCGCCCCGCGCGGTGCCGACTGGGTGGGTAAGAAATCCCTGCAAAATCAAGAAGGtttt                                                                                                                                                                                 >  2:165048/1‑251 (MQ=255)                                                    gCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCa                                                                                                                                                                            <  1:209602/251‑1 (MQ=255)                                                           aTTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATAttt                                                                                                                                                                       <  2:33582/249‑1 (MQ=255)                                                              cccGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCg                                                                                                                                                                   <  1:182071/250‑1 (MQ=255)                                                              cccGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTAGCTCTGTGTGACAAGCCGGATAACGCCCAGCGCGTTGCCGACTGGTTGGGGAAGAACTACCTGCCAAATTAGGAAGGGTTTGTTCATATTTGCCgg                                                                                                                                                                  >  1:121885/1‑251 (MQ=255)                                                                                cagcgAAGCCGATGAAGTATGTTATCGCTGAACCCGGCCTTGAACGGGTACTGCCTGGCTGCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGTCGCGGTAGCGTGCGGTATCTCCGGCCCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCACAGCGCGTTGCCGACTGGTTGGGTAAGAACTCCCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGgcgcc                                                                                                                                                <  2:219285/249‑2 (MQ=255)                                                                                          gatgaAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGGAGCGAGCGGGATCTCCGGCTCCGGCCCGACCTTGTTCCCTCTGTGTGACAAGCCGGGTAACGCCCCGCGCGTTGCCGACCGGTTGGGTAAGAACTCCCTGCAAAATTCGGGAGGTTTTTTTCATATTTGCCGGCTGGATACggcgggggggagcgtgcttt                                                                                                                                      >  2:112347/1‑237 (MQ=255)                                                                                             gaAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGGTTTGTTCATATTTGCCGGCTGGATACggcgggcggcccagtaacggga                                                                                                                                    >  1:210731/1‑236 (MQ=255)                                                                                                        tCGCTGAACCCTACCGTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGGTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGGGCAccggggcatggaaaaaaaaataa                                                                                                                         >  1:255985/1‑228 (MQ=255)                                                                                                         cGCTGAACCCTACCTTGAACGGTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAAGCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAAc                                                                                                                       <  2:210731/251‑1 (MQ=255)                                                                                                                    aCCGTGAACGGTTACTGCCTGGCTGCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTg                                                                                                            <  1:188885/251‑1 (MQ=255)                                                                                                                         gagcGGTTACTGGCTGGCTGCCGGCAGGCGCTGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGGTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCGGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGa                                                                                                       <  1:224754/248‑1 (MQ=255)                                                                                                                             ggTTACTGCCTGGCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATcac                                                                                                   <  1:112347/251‑1 (MQ=255)                                                                                                                                    gcctggCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGTAAGGTTTTGTTCATATTTGCCGGCTGTATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAATGAGc                                                                                            <  2:121885/247‑1 (MQ=255)                                                                                                                                    gcctggCTTCCGGCAGGCGCGGCAGGCGGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAATGAg                                                                                             <  1:262801/246‑1 (MQ=255)                                                                                                                                                  aggcgcggcaggcgGTTGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAATGAGCAGGTCAGCTTTgcc                                                                              <  2:1192/251‑2 (MQ=255)                                                                                                                                                        ggcaggcgGTTGCGGAAACCGGCGCGGTAGCTAGCGGTATCTCCGGTGCCTGCCCGACCTTGTTCGCTCTGTGTGACAAGCCAGATACCGCAAAGCGCGTTGCCGACTGGTTGGTTAAGAACTACCTGCAAAATCTGGAAGCTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCTCGAGTACTGGAAAACTAAATGAAACTCTACAATCTTAAAGATCACAATGAGCAGGTCAGCTTTGCGTAAGcc                                                                        <  1:267657/251‑1 (MQ=255)                                                                                                                                                                                    agcgagcgGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGTTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAg                                                                                                                                            <  1:245003/155‑1 (MQ=255)                                                                                                                                                                                      cgcgcgGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTCCCTGCAAAAGCAGGAAGGTTGTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTCCAATCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAgggg                                          <  1:80635/248‑1 (MQ=255)                                                                                                                                                                                       gagcgGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCGACAATCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAgg                                            <  1:122757/248‑1 (MQ=255)                                                                                                                                                                                                  ccggctccggcCCGACCTTGTGCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTGTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGAGCACAATGAGCAGGTCAGCTGTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTcc                               <  2:28358/250‑1 (MQ=255)                                                                                                                                                                                                             ccGACCTTGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGcc                   <  2:171742/251‑1 (MQ=255)                                                                                                                                                                                                                    tGTTCGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTGGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAAGCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAAt              <  1:60686/249‑1 (MQ=255)                                                                                                                                                                                                                        cGCTCTGTGTGACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGcc        <  1:127454/251‑1 (MQ=255)                                                                                                                                                                                                                           tctGTGTGACAAGCCGGCTACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGGACTGGGAAACTAAATGAAAATCTCAAATCTGAAAAATCACAAAGAGCAGGGCAGCTTTGCGCCAACCGTAACCCAGGGGGTGGGGAAAAAACAAGGGGTGTTTTTTTCGCACGAACTGCCGGGATTCAGActg      >  1:75136/1‑250 (MQ=255)                                                                                                                                                                                                                              gtgtgACAAGCCGGATACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAATGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCctgactg  <  2:76589/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            CGCCGCCAGGATTGCATTGCGCACGGGCGACATCTGGCAGGCTTCATTCACGCCTGCTATTCCCGTCAGCCTGAGCTTGCCGCGAAGCTGATGAAAGATGTTATCGCTGAACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTTGGGCAAAAATCAGGGGCTGTTTTTTCCGCACGACCTGCCGGAATTCAGCCTGACTG  >  NC_000913/3386‑3857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑