Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,894,839	T→C	L12P (CTC→CCC)	pabB →	aminodeoxychorismate synthase subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,894,839	0	T	C	100.0%	38.0 / NA	14	L12P (CTC→CCC)	pabB	aminodeoxychorismate synthase subunit 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/7);  total (7/7)

GGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGG‑CGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCA‑CCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGC  >  NC_000913/1894594‑1895057                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                            ggAGTGGTTGGCTCGCACCTCTTCTGCCGCCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGG‑CGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGATATCTCCCGCTGTGATTACTTTTCCCTGGc                                                                                                                                                                                                                        <  1:73317/251‑1 (MQ=255)                                                                  ccttCAGTTCCGAGATCCGCTTGACATCTTTGTGCTTCTGTTCAGTGGTGATGGAAGGTAAACCTGCAAACATTGTTAACTCCGGCTAAAT‑GTTGGGCGCTAATTATGTCATGTTACCCGTCACTTATCGATGAGAATCAGTACTGATGAAGACGTTATCTCCCGCTGTGATTACTTGACCGTGTCGGCAGTACGTTGCGGAAGTTTAATTCTCCCGCTTAAGCCA‑CCCGCCGTGCGCGATGCGTTTacac                                                                                                                                                      <  2:113089/248‑1 (MQ=255)                                                                                                               gggTGAGTGAAGGTAAAACTGCAAACATTGTTAACTCCTGCTAAATTGTTGG‑CGCTAATTATTTCATGCTACCCGGCACATCGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTACCGCTGTGCTTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCA‑CCTGCCGTGGGCGCTGCTTTTACAATCCGGCTACGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTc                                                                                                         >  1:259572/1‑251 (MQ=255)                                                                                                                     gtgaAGGTAAACCTGCAAATATTGTTAACTCCGGCTAAATTGTTGG‑CGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGGTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTGCTCCCGCTTAAGCCA‑CCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGTTTTGATATTGTGGTCgccgag                                                                                                   <  1:171884/251‑1 (MQ=255)                                                                                                                                 cTGCAAACATTGTTAACTCCTGCTAAATTGTTGG‑CGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCA‑CCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACt                                                                                       >  1:123897/1‑251 (MQ=255)                                                                                                                                    cAAACATTGTTAACTCCTGCTAAATTGTTGG‑CGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCA‑CCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACttt                                                                                     >  2:220844/1‑250 (MQ=255)                                                                                                                                                         aaaTTGTTGG‑CGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGATGTGATTACTTTACCCTGGCGTCAGGCCGCCGCTGAATTTTATTTCTCCCGCTTAAGCCA‑CCTGCCGTGGGAGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGACAcc                                                               >  1:181828/1‑251 (MQ=255)                                                                                                                                                           aTTGTTGG‑CGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCA‑CCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCGCTTTAACCACTTTCGGTAAAGTAACCgt                                                             >  1:14447/1‑251 (MQ=255)                                                                                                                                                              gttgG‑CGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCA‑CCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCgttgt                                                          <  1:50205/251‑1 (MQ=255)                                                                                                                                                                tgG‑CGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCGGGACTGATGAAGACGTTATATCCCGCTGTGATTACTTTACCCTGGCGTCAGGTCGCCTCTGACTTTTATTTCTCCCGCCTAA‑CCATCCTGCCGTGGGCGATGCTTTTACACTCCGGCTCTGCCGATCAGCCGTATTGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCgttgtc                                                         <  2:67021/250‑2 (MQ=255)                                                                                                                                                                       tAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTTAATTGTATGTCTCCCGCTTAAGCCA‑CCTGCCGTGGGCGATGCTTTTACACTCCGGCTATTCCGATCAGCCGTATAGCCGCTTTGATAGGGGGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGaa                                                                  <  1:115141/235‑1 (MQ=255)                                                                                                                                                                                  tGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGCAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCA‑CCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGCTTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGc                                       >  2:65841/1‑251 (MQ=255)                                                                                                                                                                                                         gagTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCA‑CCTGCCTTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCt                <  1:45232/251‑1 (MQ=255)                                                                                                                                                                                                                         aGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCA‑CCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCcagc  >  2:67745/1‑249 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                            GGAGTGGTTGGCACGCAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGG‑CGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCA‑CCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGC  >  NC_000913/1894594‑1895057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑