Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,860,977	C→T	W119* (TGG→TAG)	ydjL ←	putative zinc‑binding dehydrogenase YdjL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,860,977	0	C	T	100.0%	55.6 / NA	19	W119* (TGG→TAG)	ydjL	putative zinc‑binding dehydrogenase YdjL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/10);  total (9/10)

TTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGTGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTGCGCAATACAACCTGCGAACTCATGGCCGCGGATAGAGTTAAACTCATCAGAACCGCTATCGACATTGTAGTGCTTCATGTCTGCGCC  >  NC_000913/1860732‑1861215                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               ttACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGTGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCTAGGt                                                                                                                                                                                                                                            <  2:99498/250‑1 (MQ=255)                     gcCATTTGTACGGAGAACAGCCCGAGTGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCAGCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCTAGGTATTATTATCCAGACCAAGGtt                                                                                                                                                                                                                       <  1:256581/251‑1 (MQ=255)                            gTACGGAGAACAGCCCGAGTGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCTAGGTATTATTATCCAGACCAAGGTTTACtttt                                                                                                                                                                                                                >  1:43998/1‑251 (MQ=255)                                ggAGAACAGCCCGAGTGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCTAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTg                                                                                                                                                                                                            <  2:191247/251‑1 (MQ=255)                                 gagaACAGCCCGAGTGGGCCAGTGCCGATGGCGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTTGAAAAACCACCGCCCTATGTATTATTATCCAGGCCAATGTGGACTGTTTCTGt                                                                                                                                                                                                           <  1:59619/251‑1 (MQ=255)                                                                                        aTTTCGATTGCTGCGCGATGGATTTGTAGGCATTCCAGATAGGGCCAAGTACGGGTGCGTCCTCATAATCAACACCATCCGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAAACAGACACTATTTGGAAACACCACCGCCCTAGGTATCATTATCCAGACCAAGATTAACTCTTTCAGTACACCCAAGCAAATCACCTAGCTCACAGGCTGGACACACACCGCAACCGtcac                                                                                                                                                      >  2:145405/1‑246 (MQ=255)                                                                                                     cgcgATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCTAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGAt                                                                                                                                        >  1:149816/1‑250 (MQ=255)                                                                                                           ggATTTGGAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATGTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCTAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTACCCGATacgacg                                                                                                                                  <  2:46398/250‑1 (MQ=255)                                                                                                                  tAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCTAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGccc                                                                                                                          >  2:70031/1‑251 (MQ=255)                                                                                                                                  ggTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCTAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCa                                                                                                                        >  1:82988/1‑237 (MQ=255)                                                                                                                                  ggTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCTAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCa                                                                                                                        <  2:82988/237‑1 (MQ=255)                                                                                                                                                             cAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCTAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTGCGCAATACaa                                                                               <  1:268263/251‑1 (MQ=255)                                                                                                                                                                                     aCGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCTAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTGCGCAATACAACCTGCGAACTCATGGCCGCGGATa                                                       <  1:165243/251‑1 (MQ=255)                                                                                                                                                                                       gCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCTAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGCCTTTTTCACCAACCTGCGCAATACAACCTGCGAACTCATGGCCGCGGAt                                                        >  1:170242/1‑248 (MQ=255)                                                                                                                                                                                             cGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCTAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCAGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTGCGCAATACAACCTGCGAACTCATGGCCGCGGATAGAGTTaa                                                >  2:263169/1‑250 (MQ=255)                                                                                                                                                                                                    ttttGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCTAGTAATTATTATACAGACAAAGGTTTACTTTTTCAGTACAACACAGTAATTCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTAATCCGATACGACGCGTTGCACCACTTTCCAGTCTTTGACTTTTTCACCAACCTGCGCAATACAACCTGCGAACTCATGGCAGCGGATAGAGTTAAACtcatc                                         >  1:56130/1‑250 (MQ=255)                                                                                                                                                                                                                              aaaTATTTGGAAAAACCACCGCCCTAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTGCGCAATACAACCTGCGAACTCATGGCCGCGGATAGAGTTAAACTCATCAGAACCGCTATCGACAGTGTAGTGCtt              <  2:43998/250‑1 (MQ=255)                                                                                                                                                                                                                                          aaaCCCCCGCCCTAGGTAATATTAACCAGGCCAAGGTTTACTTTTTCTGTACAACACAGAAAATCCCCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTGCGCAATACAACCTGCGAACTCATGGCCGCGGATAGAGTTAAACTCATCAGAACCGCTATCGACATTGTAGTGCTTCATGTCTGcgcc  <  1:95880/251‑1 (MQ=255)                                                                                                                                                                                                                                          aaaCCACCGACCTAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGACTTTGACTTTTTAACCAACCTGCGCAATACAACCTGCGAACTCATGGCCGCGGATAGAGTTAAACTCCTCAGAACCGCTATCGACATTGTAGTGCTTCATGTCTGcgcc  >  1:191317/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               TTACCGCCCCCATAATTCGCGCCATTTGTACGGAGAACAGCCCGAGTGGGCCAGTGCCGATGACGACCACATCCTGACCAGGAAGGAATTTCGATTGCTGCGCGATGGATTTGTAGGCATTACAGATAGGGTCAAGTACGGCTGCGTCCTCATAATCAACACCATCAGGGATTTCCCACAACGCATGACGATGAATTTTGAGAATTTCACCAGGAACCAGACAATATTTGGAAAAACCACCGCCCCAGGTATTATTATCCAGACCAAGGTTTACTTTTTCTGTACAACACAGAAAATCACCTTGTTCACAGGCCGGACAAACACCGCAAACGTGACCGCTGTTATCCGATACGACGCGTTGCCCCACTTTCCAGTCTTTGACTTTTTCACCAACCTGCGCAATACAACCTGCGAACTCATGGCCGCGGATAGAGTTAAACTCATCAGAACCGCTATCGACATTGTAGTGCTTCATGTCTGCGCC  >  NC_000913/1860732‑1861215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑