Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,660,102	C→T	N678N (AAC→AAT)	ynfE →	putative selenate reductase YnfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,660,102	0	C	T	100.0%	32.6 / NA	13	N678N (AAC→AAT)	ynfE	putative selenate reductase YnfE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/7);  total (6/7)

ACGACGAACTGAAAAAAATGGGTATCTATAAGCGTAAAGATCCCAATGGCCATTTTGTCGCCTACAAAGCATTTCGTGACGACCCCGAGGCAAATCCACTTAAAACGCCTTCCGGTAAGATTGAAATTTATTCCAGCAGGCTGGCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTG‑GTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTC  >  NC_000913/1659873‑1660330                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      acgacgAACTGAAAAAAATGGGTATCTATAAGCGTAAAGATCCCAATGGCCATTTTGTCGCCTACAAAGCATTTCGTGACGACCCCGAGGCAAATCCACTTAAAACGCCTTCCGGTAAGATTGAAATTTATTCCAGCAGGCTGGCGGAAAGTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAATTCCCCTGAACGTAGAACCtt                                                                                                                                                                                                                   <  1:20846/250‑1 (MQ=255)  cgacgaACTGAAAAAAATGGGTATCTATAAGCGTAAAGATCCCAATGGCCATTTTGTCGCCTACAAAGCATTTCGTGACGACCCCGAGGCAAATCCACTTAAAACGCCTTCCGGTAAGATTGAAATTTATTCCAGCAGGCTGGCGGAAATTGCCCGTACCTGGGAACTGGAAAAATATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAATTCCCCTGAACGTAGAACCTTcc                                                                                                                                                                                                                 <  1:26272/251‑1 (MQ=255)                    gggTATCTATAAGCGTAAAGATCCCAATGGCCATTTTGTCGCCTACAAAGCATTTCGTGACGACCCCGAGGCAAATCCACTTAAAACGCCTTCCGGTAAGATTGAAATTTATTCCAGCAGGCTGGCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAATTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTG‑Gtt                                                                                                                                                                                              <  2:222827/251‑1 (MQ=255)                                                                aCAAAGCATTTCGTGACGGAACTGAGGCAAATCCACGTAAAACGCCGTCCCGTAAGATTTAAAATTATTCCAGCATGCAGGCGGAAATTGCCCATACCTGGGAACTGGAAAAAGAGGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGTCTGGAATGCCCCTTAACTTAGACCCTTCCCACTGCAACT‑TTTGTGTTTCCATTACAAATCCAGTACTCACTCGACCTAcatcaacattgac                                                                                                                                                  <  2:36423/251‑13 (MQ=255)                                                                               acgacCCCGAGGCAAATCCACTTAAAACGCCTTCCGGTAAGATTGAAATTTATTCCAGCAGGCTGGCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAATTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTG‑GTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGctgcc                                                                                                                                   >  1:141244/1‑251 (MQ=255)                                                                                 gacCCCGAAGCAAATCCACTTAAAACGCGTTCCGGTAAGATTGAAATTTATTCCAGCAGGCTGGCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAATCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAATTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTG‑GTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTTATCCCCTGAAGGctgcctc                                                                                                                                 <  2:35058/251‑2 (MQ=255)                                                                                                                  ggTAAGATTGAAATTTATTCACGCAGGCTGGCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAATTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTG‑GTTTCCATTACAAATCCCGTACTCACTCGACATACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGACAGGAGGTGTGGATCAACCCTATAGATgc                                                                                                >  2:20615/1‑251 (MQ=255)                                                                                                                                             cTGGCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAATTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTG‑GTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCg                                                                      >  1:220103/1‑250 (MQ=255)                                                                                                                                               ggCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAATTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTG‑GTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGata                                                                   <  1:145721/251‑1 (MQ=255)                                                                                                                                                  ggAAATTTCCCGTACCTGGGAACTTGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAATTCCCCTTAACGTAGAACCTTCCCACTGCAACTGTTTG‑GTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCATCCCTATAGATGCGCAGAAACGTGGGAGTGCCAACGGCGATATgg                                                                <  1:236666/251‑1 (MQ=255)                                                                                                                                                                                             aGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAATTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTG‑GTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCa                      >  1:106422/1‑250 (MQ=255)                                                                                                                                                                                                             aTGCCTCAACCTTTGAAGGCTGGAATTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTG‑GTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGCTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTAtt     >  2:8996/1‑251 (MQ=255)                                                                                                                                                                                                                gcTCAACCTTTGAAGGCTGGAATTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTG‑GTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGCGTGGATCTACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGCGGCGAAGTTCGGCTACAAGCCAAAGTAACACCACGTATtctc  >  2:150747/2‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      ACGACGAACTGAAAAAAATGGGTATCTATAAGCGTAAAGATCCCAATGGCCATTTTGTCGCCTACAAAGCATTTCGTGACGACCCCGAGGCAAATCCACTTAAAACGCCTTCCGGTAAGATTGAAATTTATTCCAGCAGGCTGGCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTG‑GTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTC  >  NC_000913/1659873‑1660330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑