Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,632,513	C→T	intergenic (‑228/‑377)	ydfJ ← / → ynfT	putative transporter YdfJ/protein YnfT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,632,513	0	C	T	100.0%	14.7 / NA	7	intergenic (‑228/‑377)	ydfJ/ynfT	putative transporter YdfJ/protein YnfT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/3);  total (4/3)

AACTGGAAATCCATAATTCACAGGTGTTTTTTCCCATCCTGTGGTTTCCTTGGCGTTTTCTAGGTTTTTTCAGATAGTTGCATTTTTTTAAAAAGCATCCTAAGTTCGATCTCAGTGTCTATCTGGGGCCTATTTCTGTCCCATATATGCCCCAAAAAAACTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATCGAAAAATTTACCAGCCAATCGCAGCACGTTCTTGCATAAGGTGTGTCTGCGGTTTTTCAACTATTCAGATACATCACTCCCATCACATTCATTCCTCCGCATCAAAGGCATATAGGCTATATCACCTTGATATTTTTC  >  NC_000913/1632272‑1632651                                                                                                                                                                                                                                                  |                                                                                                                                           aaCTGGAAATCCATAATTCACAGGTGTTTTTTCCCATCCTGTGGTTTCCTTGGCGTTTTCTAGGTTTTTTCAGATAGTTGCATTTTTTTAAAAAGCATCCTAAGTTCGATCTCAGTGTCTATCTGGGGCCTATTTCTGTCCCATATATGCCCCAAAAAAACTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATTGAAAAAttt                                                                                                                                   >  2:54963/1‑251 (MQ=255)         aTCCAGAATTCACAGGTGTTGTTTCCCATCCTGGGGTTTCCTTGGCGTTTTCTAGGTTTTTTCAGATAGTTGCATTTTTTTAAAAAGCATCCTAAGTTCGATCTCAGTGTCTATCTGGGGCCTATTTCTGTCCCATATATGCCCCAAAAAAACTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATTGAAAAATTTACCAGCCa                                                                                                                           <  1:124292/251‑1 (MQ=255)                     caGGTGTTTTTTCCCATCCTGTGGTTTCCTTGGCGTTTTCTAGGTTTTTTCAGATAGTTGCATTTTTTTAAAAAGCATCCTAAGTTCGATCTCAGTGTCTATCTGGGGCCTATTTCTGTCCCATATATGCCCCAAAAAAACTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATTGAAAAATTTACCAGCCAATCGCAGCACGt                                                                                                               >  2:93034/1‑251 (MQ=255)                                                                                                   ccTAAGTTCGATCTCAGTGTCTATCTGGGTCCTATTTCTGTCCCATATATGCCCCAAAAAAACACCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATTGAAAAATTTACCAGCCAATCGCATCACGTTCTTGCATAAGGTGTGTCGGCGGTTTTTCAACTATTCAGATACATCACTCCCATCACATTCATTCCTCCGCATCAAAg                                 <  1:10188/251‑1 (MQ=255)                                                                                                            gATCTCAGTGTCTATCTGGGGCCTATTTCTGTCCCATATATGCCCCAAAAAAACTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCATACTATTGAAAAATTTACCAGCCAATCGCAGCACGTTCTTGCATAAGGTGGGTCTGCGGTTTTTCAACTATTCAGATACATCACTCCCATCACATTCATTCATCCGCATCAAAGGCATATAgg                        >  2:205137/1‑251 (MQ=255)                                                                                                                                gCCTATTTCTGTCCCATATATGCCCCAAAAAAACTCCCCAACAGAAAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATTGAAAAATTTACCAGCCAATCGCAGCACGTTCTTGCATAAGGTGTGTCTGCGGTTTTTCAACTATTCAGATACATCACTCCCATCACATTCATTCCTCCGCATCAAAGGCATATAGGCTATATCACCTTGATAtttt    <  2:121952/251‑1 (MQ=255)                                                                                                                                  cTATTTCTGTCACCTCTATGCCCCAAAAAACCTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATTGAAAAATTTACCAGCCAATCGCAGCACGTTCTTGCATAAGGTGTGTCTGCGGTTTTTCAACTATTCAGATACATCACTCCCATCAAATTCATTCCTCCGCATCAAAGGCATATAGGCTATATCACCTTGATAtttttc  >  1:210025/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                           AACTGGAAATCCATAATTCACAGGTGTTTTTTCCCATCCTGTGGTTTCCTTGGCGTTTTCTAGGTTTTTTCAGATAGTTGCATTTTTTTAAAAAGCATCCTAAGTTCGATCTCAGTGTCTATCTGGGGCCTATTTCTGTCCCATATATGCCCCAAAAAAACTCCCCAACAGATAAGTAGTTTTTTCATGGATTTATGCGTAAAATCAAGAACGGCTGGAAATCATTCAATACTCACACTATCGAAAAATTTACCAGCCAATCGCAGCACGTTCTTGCATAAGGTGTGTCTGCGGTTTTTCAACTATTCAGATACATCACTCCCATCACATTCATTCCTCCGCATCAAAGGCATATAGGCTATATCACCTTGATATTTTTC  >  NC_000913/1632272‑1632651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑