| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,629,247 | C→T | I11I (ATC→ATT) | ydfZ → | putative selenoprotein YdfZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,629,247 | 0 | C | T | 93.3% | 35.4 / ‑3.8 | 15 | I11I (ATC→ATT) | ydfZ | putative selenoprotein YdfZ |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (8/6); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.86e-01 | |||||||||||
CTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACC‑TATGACGACCTACGATCGTAACCGTAACGCAATCACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCAT > NC_000913/1629084‑1629493 | cTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACC‑TATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCa > 1:73313/1‑250 (MQ=255) aTGGCACATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACC‑TATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATc < 2:73313/251‑1 (MQ=255) ggCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAAGATTTCTCTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGTTTCGACTGTAAATCAGAAATGAGAAAACACC‑TATGACGACCTTCGAGCGTAACCTTAACGCAATTGCCACTGGCTGCCTGGTGATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATcc < 1:252980/250‑1 (MQ=255) aGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACC‑TATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGt > 1:14501/1‑251 (MQ=255) gCTTGATAAAAAACAAAAAATATTTCACTAGACAGGAGAATTTATATTGCGCCCGTTACGTGGGCTTCGACTGAAAATCAGAAAGGAGAAAACACC‑TATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGCTCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGtt > 2:215312/1‑251 (MQ=255) tGATCAAAAACAAAAAAAATTGCTCTCGACAGGAGTAGTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACC‑TATGACGACTTACGAGCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCTGTCACACTGGCAAGATCCTGTCGATTAATACTGAAGGTCTGACCGCTTAGCAAATCCGCCGCGGAAAAACCGTAgtttgttaaggtggt < 2:233936/250‑13 (MQ=255) tatTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTAGGCTTCGACTGTAAATCGGAAAGGAGAAAACACC‑TATGACGCCATACGAACGTACCCATAACGCAATTACCCCTGGCAGCCGTGTTATGGTTAGAGGCACCGGTCCCACTGGCAAGATCATGTCGATTGATACTGAAGGTCTGACCGCTGCACAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTAGTAAAGAGAAACTGGaacc > 1:15605/1‑250 (MQ=255) cGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACC‑TATGACGACCTACGATCGTAACCGTAACGCAATCACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGAcc < 1:32359/249‑1 (MQ=255) tttATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACC‑TATGACGACCTTCGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGAcc < 1:146659/168‑1 (MQ=255) tttATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACC‑TATGACGACCTTCGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGAcc > 2:146659/1‑168 (MQ=255) agaagaaaCACCCTATGACGACCTACGATCGTAACCTGACCGCAATTCCCCCGGGCAGCCGTGTTATGGTTAGCGCCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTGTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTTAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCttt < 2:144089/246‑1 (MQ=255) aaCACC‑TATGACGACCTACGCTCGTAACCATAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACtt > 1:217529/1‑251 (MQ=255) tATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACt > 1:95712/1‑100 (MQ=255) tATGACGACCTACGATCGTAACCGTAACGCAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACt < 2:95712/100‑1 (MQ=255) cAATTACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCAt > 1:30283/1‑251 (MQ=255) | CTTATGGCTCATGCATGCATCAAAAAAGATGTGAGCTTGATCAAAAACAAAAAATATTTCACTCGACAGGAGTATTTATATTGCGCCCGTTACGTGGGCTTCGACTGTAAATCAGAAAGGAGAAAACACC‑TATGACGACCTACGATCGTAACCGTAACGCAATCACCACTGGCAGCCGTGTTATGGTTAGCGGCACCGGTCACACTGGCAAGATCCTGTCGATTGATACTGAAGGTCTGACCGCTGAGCAAATCCGCCGCGGAAAAACCGTAGTTGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCAT > NC_000913/1629084‑1629493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |