Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	867	C→T	S177S (AGC→AGT)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	867	0	C	T	100.0%	32.1 / NA	13	S177S (AGC→AGT)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/0);  total (13/0)

CAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCT  >  NC_000913/622‑1106                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                cAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCAt                                                                                                                                                                                                                                            >  2:225840/1‑251 (MQ=255)    gAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGATGCGCTGATTTGCCGTGGCGAGAACATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCAGGCAGTGGGGCATTACCTCGAATCTACCGACGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTcc                                                                                                                                                                                                                                         >  1:160064/1‑251 (MQ=255)                     aCATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGc                                                                                                                                                                                                                        >  1:59395/1‑251 (MQ=255)                       aggtacTGCAAGGCATTAGGTTGTTGGGGCAGTGCCCGGATAGAATCAACGCTGCGCTGATTTGCCGTGGCGAGAAGATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTAACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGACGGCTTGCGGCAAGTCGCATTCAGGATGATCACATGGTGCTg                                                                                                                                                                                                                      >  2:236889/6‑251 (MQ=255)                          tCCTGCATGGCATTAGTTTGGTTGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGGATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTCCCGTCGATATTGCTGCGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGATGATCACATGGTGCTGATg                                                                                                                                                                                                                   >  2:3377/1‑251 (MQ=255)                           ccTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCAACCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATgg                                                                                                                                                                                                                  >  1:61806/1‑251 (MQ=255)                                                                                            gCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACATCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGGACTTGGACGCAACGGTTCCGacta                                                                                                                                                  >  2:202399/1‑250 (MQ=255)                                                                                                                           cGTATTAGCAGCGCGCGGTCACTACGTTACCGTTCTCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGCGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTActccgcg                                                                                                                                           >  2:127266/1‑222 (MQ=255)                                                                                                                                                caACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGAATTgg                                                                                              >  1:9183/1‑250 (MQ=255)                                                                                                                                                    gTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTACGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGCGATTTggacgg                                                                                          >  1:250914/1‑250 (MQ=255)                                                                                                                                                                                                      cGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTACGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGCCGGCCGTTGACGGGGGCTATACCTGCGGCCCGCGTCAGGTGCCCGATGCGAGgt                                       >  1:20979/1‑251 (MQ=255)                                                                                                                                                                                                             aCCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTT‑ACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGa                                 >  1:135321/1‑249 (MQ=255)                                                                                                                                                                                                                                           aTTGCGGCAAGTCGCATTCCGGCTGATCACATGGAGCTGATGGCAGGTTTCACCGCCGGTAATGACAAAGGCGAACTGGTGGAACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGAGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGCCAGGTGCCCGGTGCGAGGTTGTTGTAGTCGATGTCCTACCAGGAAGCGATGGaact  >  1:117149/1‑248 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                CAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCT  >  NC_000913/622‑1106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑