| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,375,737 | G→A | A259T (GCC→ACC) | ycjQ → | D‑guloside 3‑dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,375,737 | 0 | G | A | 90.5% | 48.4 / ‑4.8 | 21 | A259T (GCC→ACC) | ycjQ | D‑guloside 3‑dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (10/9); minor base T (2/0); total (12/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
GCTAAACGCGCTGGCGCTTCTGTGGTGATTGGCGTCGATCCTATCGCCCATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTGCCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCA > NC_000913/1375491‑1375978 | gCTAAACGGGCTGGCGCTTCTGTGGTGATTGGCGTCGATCCTATCGCCCATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCaa < 2:36843/251‑1 (MQ=255) gcgcTGGCGTTTCTGTGGTGATTGGCGTCGATCCTATCGCCCATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCAGCTCCTATGTCGCGTTTACCAAGCCGttt < 2:53161/251‑1 (MQ=255) tGGCGATTCTGTGGTGATTGGCGTCGATCCTATCGCCAATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCCCCGCGGTCTGGCTTATGGCGGCACCATCTCCTTTGTCGCGTTTAACAAGCCGTTTgacg > 2:187096/1‑248 (MQ=255) ttCTGTGGTGATTGGCGTCGATCCTATCGCCCATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATATCCTATGGCGGGTTTACCAAGCCGTTTGCCGAAGGtt > 2:1243/1‑251 (MQ=255) cGTCGATCCTATCGCCCATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGGGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGCCTTATGGCGGCACCATCTCCTATGTCCCGTTTACCAAGCCGTTTGCCGCACGTTTTAACCTCGGAcgcg > 2:192138/1‑251 (MQ=255) aTCGCCCATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCAtt < 1:130798/250‑1 (MQ=255) atatTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCACAACGATGAACGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCAGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCAACATCTCCTTTGTCGCGGTTTCCAACCCGTTTGACCAAGGTTTTAACCTCGGACACGAAGAGCCTTTCCATAACGCCaaactt > 1:126290/1‑248 (MQ=255) atatTGCCCGTCGCCACGGCGCGGAGTTCTGCCGTAACCCCAGCGGCACTGATGTAGGTAAAGAGATCAAAACTCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCCCGGGCTGGCTTATGGCGGCACCAGCTCCGATGTCGCGTTTACCAATCCTTTTGCCGAAGGTTTTAACTTCGGGCGCGAAGCGCGTTTCAATAACGCCtaaagt < 2:242544/251‑3 (MQ=255) gcgcGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAACCGTTTGCCGAAGGGTTTAAACTCGGACGCGAACCGCATTTCAATAACGCCAAAATTGTCTTTTCTCGCCCGTGc > 2:149440/1‑251 (MQ=255) ggATTTTTGACTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGTTGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGt > 2:109671/1‑176 (MQ=255) ggATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCGGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGt < 1:109671/176‑1 (MQ=255) ggATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGAGTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTAGGGCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAGGCGCATTTCAATAGCTCCAAAATTGTCTTCTCTCGCGCGTGCAGcg < 1:103590/251‑1 (MQ=255) agaTCAAAACGCTGACCGGCAAGCAGGGTGCCGATGAGATTATCGAAACCAGCGGCTACGCCGACGCGCTACAATCGGCGCCCCGAGGTCTGGCTTATGGCGGCACCATCTCCTATGTCACGTTTACCAAGCAGTTTGCCGAAGGTGTTGAACTCGGACGCGCAGCGCATTTCAATAACGCCCAAATTGTCTTATCTAGCTCGTGCCGCGAACCGAACCCGGATTATCCGCGATGGAGCCGCAAGCGTAtt > 2:259208/1‑251 (MQ=255) aaaCGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAAACGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTgaagaa < 2:61331/251‑1 (MQ=255) gTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGGTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGAGTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCtgatg > 1:39931/1‑251 (MQ=255) tGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGGATTGAAGAAACCTGTTGGGAACTGCtgatg > 1:174835/1‑250 (MQ=255) gAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTg < 2:77163/251‑1 (MQ=255) aTCGGCGCTCCGCGGTCTGACTTATGGCGTCACCATCTCCTATGTCGCGTTTTCCAAGCCGTTTGCCGCAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCCGCGAACCGAACCCGGATTCTCCGCGCTGGGGCCGCAAGCGTATTTAAGAAACCTGTTGGGAAATGCTGATGAACGGGTATCTCCATTGCAAAGATTTAATCGACCCGGTGGGGACCt > 2:132278/1‑251 (MQ=255) tCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGCACCCGGATTATCCGCGCTGGAGCCGCAAGCGGATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGGGACCTTTGCCAAt > 1:14497/1‑251 (MQ=255) aTCTCCTATGTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGt > 1:88883/1‑251 (MQ=255) gTCGCGTTTACCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCa < 2:174835/251‑1 (MQ=255) | GCTAAACGCGCTGGCGCTTCTGTGGTGATTGGCGTCGATCCTATCGCCCATCGCTGTGATATTGCCCGTCGCCACGGCGCGGATTTCTGCCTTAACCCCATCGGCACTGATGTAGGTAAAGAGATCAAAACGCTGACCGGCAAGCAGGGTGCCGATGTGATTATCGAAACCAGCGGCTACGCCGACGCGCTGCAATCGGCGCTCCGCGGTCTGGCTTATGGCGGCACCATCTCCTATGTCGCGTTTGCCAAGCCGTTTGCCGAAGGTTTTAACCTCGGACGCGAAGCGCATTTCAATAACGCCAAAATTGTCTTCTCTCGCGCGTGCAGCGAACCGAACCCGGATTATCCGCGCTGGAGCCGCAAGCGTATTGAAGAAACCTGTTGGGAACTGCTGATGAACGGTTATCTCAATTGCGAAGATTTAATCGACCCGGTGGTGACCTTTGCCAATAGCCCGGAAAGCTATATGCAGTATGTCGACCAGCA > NC_000913/1375491‑1375978 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |