Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,358,8590TC81.8% 17.2 / ‑5.1 11Y110C (TAT→TGT) puuPputrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (3/6);  minor base A (1/1);  total (4/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

CCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCA  >  NC_000913/1358622‑1359102
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   
ccAACGCATTCTCATTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGAAGAAGGGTAACACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGAAAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCgg                                                                                                                                                                                                                                        >  2:198293/1‑250 (MQ=255)
                            gAACTACCAGGAAGATAAACGCCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGTCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTg                                                                                                                                                                                                             <  1:12948/250‑1 (MQ=255)
                                          aTAAACCCCACCATTATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACtt                                                                                                                                                                                              <  1:226448/251‑1 (MQ=255)
                                                                        aGTACAAGCAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGCCGAAGGTTACCACCCACACCCACCGCGGCACTTCCGGGGAGAGGGCGGCGAGATAGATTTTTGCCAACAAGAAGATAATCATCGGCAAAAAGAGACAATCCagcagc                                                                                                                                                                                                                                          >  1:230049/1‑177 (MQ=255)
                                                                        aGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCagcagc                                                                                                                                                                                                                                          <  2:230049/177‑1 (MQ=255)
                                                                                                                          cgcgGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAAGCGACTTTTGCGCGGAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCacac                                                                                                              <  1:169369/251‑1 (MQ=255)
                                                                                                                                     aaaTGGCGACGAACGTTACCAACCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGAAAATCCAGCAGCGATGACCAGCCGACCATACATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGCACCGGCCTCCGGAAAATGGCGAACCAGTTTGCCGTAGATGATAGCgggcaaccgaaaacccgcccgagcc                                                                                                   >  2:51588/1‑228 (MQ=255)
                                                                                                                                            gacgaAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAAt                                                                                            >  1:42069/1‑251 (MQ=255)
                                                                                                                                                                                                 aatagatTTCCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAACCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGTTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTGTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCa                                       <  1:95521/250‑1 (MQ=255)
                                                                                                                                                                                                                acaaGACGTTAATCATCGGCAAAAAGAGACAACCCAGCAGCGATGCCCAGCAGACCATAAAGCCGACGGGCGGGTTAATCGACTTTGGCGCGTAGGTATAGTCCGACCCGGCCCCCGGAAACTTGCGAACAAGTTTGCCGTAGTTGATAGCGGTAAACAGCACATCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGGATCAAATACGGTCATCGGc                        <  1:46115/251‑1 (MQ=255)
                                                                                                                                                                                                                                      aaaGAGAAAATCCAGCAGCCAGGCCCAGCCGACCATAAACCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGGATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTGGCCGTAGCTGATAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCcatca  <  1:195365/251‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   
CCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCA  >  NC_000913/1358622‑1359102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑