Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,341,542	G→A	A329T (GCG→ACG)	lapB →	lipopolysaccharide assembly protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,341,542	0	G	A	100.0%	74.3 / NA	25	A329T (GCG→ACG)	lapB	lipopolysaccharide assembly protein B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/14);  total (11/14)

GTCAGCGAAACGCTGGAAATGTTGCAAACCTGCTATCAGCAGTTGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAA  >  NC_000913/1341299‑1341757                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                        gTCAGCGAAACGCTGGAAATGTTGCAAACCTGCTATCAGCAGTTGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACTGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGgaaga                                                                                                                                                                                                                  <  2:198865/251‑1 (MQ=255)  tCAGCGAAACGCTGGAACTGTTGCAAACCTGCTCTCAGCCGTTGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGTTTACCACTTAAATGAAACGgaaga                                                                                                                                                                                                                  >  2:40681/1‑250 (MQ=255)   cagcggaccgctggacctgtttaaaaCCTTCTATCAGCAGTTGGGTACAACTACCGCATGGGCAGAATTCCTGCAGCGTGTGGTGGAAGAGACCACAGGTACCGATGCTGACTTGATGCTTGCTGATATCACCGAAGCGCGCGACGGAAGTGAGGCAGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGCTTACAACTTAAATGAAACGgaaga                                                                                                                                                                                                                  >  2:266434/24‑249 (MQ=255)                             ccTGCTATCAGCAGTTGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGtg                                                                                                                                                                                      >  1:124376/1‑251 (MQ=255)                                    tCAGCAGTTGGGTAAAACTGCCGAATGGGCCGAATTCCTGCAGCGTGCGGAGGACGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGCTCTCATCGCAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTCAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTg                                                                                                                                                                               >  2:258599/1‑251 (MQ=255)                                          gTTGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACCCCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTGAATGGATTACCACTTAAAGGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATgg                                                                                                                                                                         <  1:267876/251‑1 (MQ=255)                                                               ggCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACgtagt                                                                                                                                                     >  1:84953/1‑250 (MQ=255)                                                                    aaTTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCt                                                                                                                                               >  2:84781/1‑251 (MQ=255)                                                                           gCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTa                                                                                                                                          <  2:22402/249‑1 (MQ=255)                                                                            cAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCg                                                                                                                                       <  1:48997/251‑1 (MQ=255)                                                                                       ggAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATAGTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAAGGGATGACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTAGCGTTGCCAGAAAt                                                                                                                            <  2:18048/251‑1 (MQ=255)                                                                                        gAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCAGCGAAGCGCGCGACGGTAGTGAGGCCGCACATGTCTATATTACGCGCCAGCGTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGGTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGGTGGCGAGAAGGTACGGAGTAAGCCTCGTTATCGTTGCCAGaaagg                                                                                                                           <  2:57180/251‑3 (MQ=255)                                                                                                 acCGGTGCCGATTCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGTTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGGTTTAc                                                                                                                  <  1:48429/251‑1 (MQ=255)                                                                                                       gCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCAt                                                                                                             <  1:132284/250‑1 (MQ=255)                                                                                                                          ttgctGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGt                                                                                         >  1:164072/1‑251 (MQ=255)                                                                                                                                        gAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGc                                                                           >  2:232693/1‑251 (MQ=255)                                                                                                                                                                          atatTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCtt                                            <  1:258599/248‑1 (MQ=255)                                                                                                                                                                                         ttCAGAGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGAAATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAAttttt                           >  2:131491/1‑250 (MQ=255)                                                                                                                                                                                              cGTCATCCGACCATGCGTGTGTTCCATAAGTTACTGGCTTACCAATTAAATGAAACGGAAGAAGGGCGTGACACAGCGAGCCTGATGGTGCTGCGTGACATGCTTGGCGAGAAGGTACGTAGTACGACTCGTTATCGTTGCCAGACATGTGGTTTTACCGCATACACCCTCTCCTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTTACCCGTTTCGCGGTCTTGATGGCCTGTTATTTTTaaaat                      >  2:128884/1‑249 (MQ=255)                                                                                                                                                                                               gTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGTGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTaaaaaaa                    <  1:148114/251‑1 (MQ=255)                                                                                                                                                                                                    ccGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATcc                <  1:232693/250‑1 (MQ=255)                                                                                                                                                                                                        ccATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACtt           <  1:40681/251‑1 (MQ=255)                                                                                                                                                                                                        ccATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCACACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACtt           >  2:193220/1‑251 (MQ=255)                                                                                                                                                                                                              gtgtgtTCCATAATTTAATGGATTACCACTTAAATGAAACGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGTGCCTGGTCATCCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTGTAAAAAAATCCGACTTTAGTTa     <  2:128558/251‑1 (MQ=255)                                                                                                                                                                                                                  gtTCCATAAGTTAATGGATTACCACTTAAATGAAACGGATGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTacaa  <  2:164072/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                        GTCAGCGAAACGCTGGAAATGTTGCAAACCTGCTATCAGCAGTTGGGTAAAACTGCCGAATGGGCAGAATTCCTGCAGCGTGCGGTGGAAGAGAACACCGGTGCCGATGCTGAATTGATGCTTGCTGATATCATCGAAGCGCGCGACGGTAGTGAGGCCGCACAGGTCTATATTACGCGCCAGCTTCAGCGTCATCCGACCATGCGTGTGTTCCATAAGTTAATGGATTACCACTTAAATGAAGCGGAAGAAGGGCGTGCCAAAGAGAGCCTGATGGTGCTGCGTGACATGGTTGGCGAGAAGGTACGTAGTAAGCCTCGTTATCGTTGCCAGAAATGTGGTTTTACCGCATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAA  >  NC_000913/1341299‑1341757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑