Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,323,798	G→A	K193K (AAG→AAA)	rnm →	RNase AM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,323,798	0	G	A	100.0%	37.7 / NA	15	K193K (AAG→AAA)	rnm	RNase AM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/7);  total (8/7)

CGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAGGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGT  >  NC_000913/1323581‑1324043                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      cgcAAATTCCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCtt                                                                                                                                                                                                                      <  2:63674/251‑1 (MQ=255)     aaTACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCGTAGTg                                                                                                                                                                                                                  <  1:222418/251‑1 (MQ=255)                 gcTGGAAGGCGCACAACGACTGGCGCATGGCGGCGCAGTGACGGGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTGAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATGGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCGGGGCGGGACAATCTTAGTGCTAAATGGCTGa                                                                                                                                                                                                      <  2:89068/251‑1 (MQ=255)                                                        gactcgcgGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTGATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAAGCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCAGTTTGCCGAACACCACGGTGAc                                                                                                                                                                <  2:65654/246‑1 (MQ=255)                                                                gTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGa                                                                                                                                                        >  1:180128/1‑250 (MQ=255)                                                                                                                 ggATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGccc                                                                                                      >  2:62652/1‑251 (MQ=255)                                                                                                                                    cTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGCCAGCAACAttt                                                                                   >  1:225675/1‑251 (MQ=255)                                                                                                                                             gggAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTAGTTATGTCATTCAGCATTCTGGCGGTAAAGCGGGGCTAGCGCATCCTGGGCGGTACAATCTTAGTGCTGAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACTGTGACGCGATGGAAGTCGCGCAGGGCCAGCTATCGCCCGATGAACGGGCCCAGCTGGCGTCTCTTGCGCGTCAGCATCAGTTATGGGcttc                                                                          <  2:138338/251‑4 (MQ=255)                                                                                                                                                     cGGATACGTTCCGCCACAGTGGTGTACAATAGGCCCAGCTATTGATGTACTTCATCATTCTGGCGGTAAAGAGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGCACACCACGGAGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAACTGGCGGCCCTTGCGCGTCAGCATCATTTACGGGCATCACTAGGAt                                                                  >  2:141183/1‑251 (MQ=255)                                                                                                                                                                                   aGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCg                                    <  1:1837/251‑1 (MQ=255)                                                                                                                                                                                                    tcattcatCATTCTGGCGGTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAActc                   >  1:131973/1‑251 (MQ=255)                                                                                                                                                                                                                  ggCGGTAAAGCGGTGCTAGCTCGTCCTGGGCGTTACAATCGTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCATCATCATTTAGGGGCATCACAAGGATCTGAGTGTCATCAGCCATTGCCGTGGATCGAACTGGGTCggatactcgtgcttcccgc        <  2:180128/248‑19 (MQ=255)                                                                                                                                                                                                                     ggTAAAGCGGTGCTAGCTCATCCTGGGCGGTACCATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGt  >  2:124969/1‑251 (MQ=255)                                                                                                                                                                                                                     ggTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCACTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGt  >  1:36623/1‑251 (MQ=255)                                                                                                                                                                                                                     ggTAAAGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGCGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGGGACGCGATGGAAGTAGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCCTGCCCGTGGATCGACATGGGGCGTAAACTCTGGCAGCCCGCAGGCGt  >  2:163832/1‑251 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                      CGCAAATACCTGGGGCGCTGGAAGGCGCACAACGACTGGCGCAGGGCGGCGCAGTGACGCGCGGTCATTTTGCGCGTTTCCTTGTTGAGTGCGGCAAAGCCAGTTCAATGGCGGATGTCTTTAAAAAGTATCTGGCGCGCGGGAAAACCGGATACGTTCCGCCACAGTGGTGTACAATAGAACAAGCTATTGATGTCATTCATCATTCTGGCGGTAAGGCGGTGCTAGCTCATCCTGGGCGGTACAATCTTAGTGCTAAATGGCTGAAAAGATTGGTTGCGCATTTTGCCGAACACCACGGTGACGCGATGGAAGTCGCGCAGTGCCAGCAATCGCCCAATGAACGTACCCAGCTGGCGGCCCTTGCGCGTCAGCATCATTTATGGGCATCACAAGGATCTGATTTTCATCAGCCATGCCCGTGGATCGAACTGGGTCGTAAACTCTGGCTGCCCGCAGGCGT  >  NC_000913/1323581‑1324043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑