| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,282,857 | C→T | R998R (CGC→CGT) | narG → | nitrate reductase A subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,282,857 | 0 | C | T | 95.2% | 52.5 / NA | 21 | R998R (CGC→CGT) | narG | nitrate reductase A subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (11/9); minor base G (1/0); total (12/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCC > NC_000913/1282617‑1283100 | cTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACCCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAgg > 2:59156/1‑251 (MQ=255) tGGAACACCCAGAGCGAGATGGATATGCTGCGTAAGCTCATCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCAGATGCTGCACACCGCAATTGATGCGGCAGAGATGCTCCTGCCCCTGGCCCCGGAAACCAACGGTCAGGTAGACGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGCAGATCCGCTTCCGGGAGATTCAGGc > 2:259728/1‑251 (MQ=255) ggATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTAtc < 1:4353/250‑1 (MQ=255) gAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGACTGGTCTGGAAGATGAACACGTTTCTTAc > 2:253103/1‑250 (MQ=255) ccAGCCGATGCTGAACCCCGCAATTGATGCGGCAGAGAAGATCCTGTCACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACTCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGTTCTGGAAGATGAACACGTTTCTTACAACGCCg < 2:173653/251‑1 (MQ=255) ccGATGCTGAACACCGCAATTTATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTTAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTTAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGtga < 1:255704/251‑3 (MQ=255) cGATGCTGAACACCGCAATTGATGCTGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAAGTTACCGGTCGGGACCATACGCATCTGGCGCTGAATAAATAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGTAATATTCACACGTTTCTGACAACGCCGGTTac < 2:258235/251‑1 (MQ=255) acCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTc > 1:70120/1‑250 (MQ=255) tGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGAAGCCGTGAAAGCCTGGGCTGCCCCGCACGCATTTACCGCTCGTGACCATACGCATATGCCGCTGAATAAAGAAGCCGAGACGATCCGCTTCCGTGATATTCAAGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACCCCAAAGTTCACGAGCTg > 1:127896/1‑250 (MQ=255) tGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAGGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACTTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctatct > 2:74646/1‑251 (MQ=255) ggCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgtc < 2:104524/251‑1 (MQ=255) ggTCAGGTAACCGCGACAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCCTACGCCTCTGGCGCTGAATAAAGCAGACGCGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAgg > 2:264421/1‑250 (MQ=255) gTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGTTCGTCAGCAACTGTCTCAGGAt > 2:217656/1‑251 (MQ=255) aaaGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCg > 2:92923/1‑251 (MQ=255) gTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAg < 1:135508/200‑1 (MQ=255) gTGACAATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAg > 2:135508/1‑200 (MQ=255) tGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgc > 2:264798/1‑251 (MQ=255) gACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTccgcc < 1:92923/251‑1 (MQ=255) gcTGAATAAAGAAGACGATAAGATCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGTCGTTCTCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGAGTTCGTTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCgg < 1:259728/251‑1 (MQ=255) agaagaGCCGCTTCCGTGATATTCAGGCACAGCCGCGCAAAATTATCTCCATCCCTACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGATCTGATCCTATGGCGTACGCTCTCTTGTCGTCAGCAACTGTATTAGGATCACCAGTTTATGCGTGATTTCGGTGAAAGCCGGCTGGTTGATCGTCCGCCGATCGACACCCGTTCGGTTAAAGAAGTGATAGGc < 1:158518/251‑1 (MQ=255) gCTTCCGTGATATTCCGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAAAGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATcc > 2:180595/1‑251 (MQ=255) | CTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACCAGTGGATGCGTGATTTCGGTGAAAGCCTGCTGGTTTATCGTCCGCCGATCGACACCCGTTCGGTGAAAGAAGTGATAGGCCAGAAATCC > NC_000913/1282617‑1283100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |