Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	774	T→C	T146T (ACT→ACC)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	774	0	T	C	100.0%	74.6 / NA	27	T146T (ACT→ACC)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/6);  total (21/6)

GCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGG  >  NC_000913/530‑1018                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     gCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATc                                                                                                                                                                                                                                                <  1:204942/251‑1 (MQ=255)       ccGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCCGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCCGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGACAATGTCGATCGCCATTATGGCCGGCGTCTTAGAAGCGCGCGGTCACCACGTTACCGTTATCGATCCg                                                                                                                                                                                                                                          >  1:115924/1‑251 (MQ=255)       ccGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAACATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCg                                                                                                                                                                                                                                          >  1:107211/1‑251 (MQ=255)         gAACGTATTTTTGCCGGACATTTGACGGGACTCGCCGCCCCCAAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTCGTTTGTTGGTGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGCTCGCCATTATGGCCGGCGAATTAGAAGCGAGCGGTGACAACGTTACCGTTATCGATCCGGt                                                                                                                                                                                                                                        >  2:154458/1‑251 (MQ=255)             gTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGa                                                                                                                                                                                                                                     >  2:49906/1‑250 (MQ=255)                 ttttGCCGAACTTTAGACGGGACACGCCGCCACCCAGCCGGTATTCCCGCGTGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACAGGTCCTGCATGGCATTAGTTTGTTGGGGAAGTGCCCGGATAGCATCAACGCTGCGATGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGAGTATAAGAAGCGCGCGGTCACAACGCTACCGTGATCGATCCGGTCGaaaaa                                                                                                                                                                                                                                 <  2:116622/250‑1 (MQ=255)                  tttGCCGAACTTTTGACGGGAATCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAAct                                                                                                                                                                                                                               >  1:190389/1‑251 (MQ=255)                    tGCCGAACTTTTGACGGGACTCGCCGCCGCCCCGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAACTAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAActgc                                                                                                                                                                                                                             >  1:221229/1‑251 (MQ=255)                              ttGACGGGACTCGCCGCCGCCCAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTgg                                                                                                                                                                                                                    <  2:63484/250‑1 (MQ=255)                                   gggccTCGCCGCCGCACAGCCGGGAGTCCCGCTGGCGCAGTTGAAATCTTTCGTCGAACAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATGAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGAGCGCCATTTTGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCagt                                                                                                                                                                                                              <  2:258596/247‑3 (MQ=255)                                     gACTCGCCGCCGCCCAGCCGGGATTCCCGCGGGCGCAATTGCAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGGTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGCAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACCACGTTACCGTTATCGATCCGGACGACAAACTGCTGGCAGTGGGGCATTAc                                                                                                                                                                                                            >  1:18218/1‑251 (MQ=255)                                                   cAGCCGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGt                                                                                                                                                                                              >  1:175880/1‑251 (MQ=255)                                                      ccGGGATTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGa                                                                                                                                                                                           <  2:190653/251‑1 (MQ=255)                                                                   tGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGTCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTcc                                                                                                                                                                              >  1:160789/1‑251 (MQ=255)                                                                     gcgcAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATCAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTcccc                                                                                                                                                                            >  2:164034/1‑249 (MQ=255)                                                                      cgaaaTTGAAAACTTGCGTCGATCAGGAATTTGCCCAAAGAAAACATGTCCTGTATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGTCCGGCGTAGTAGAAGCGCTCGGTCCCAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGTGGCATTACCTCGAATCTATCGTCGATATTGCTGAGTcctcc                                                                                                                                                                           <  2:246942/248‑4 (MQ=255)                                                                            tGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCCTCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGt                                                                                                                                                                     >  1:33188/1‑251 (MQ=255)                                                                                             cAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCAt                                                                                                                                                    >  2:225840/1‑251 (MQ=255)                                                                                                gAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGATGCGCTGATTTGCCGTGGCGAGAACATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCAGGCAGTGGGGCATTACCTCGAATCTACCGACGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTcc                                                                                                                                                 >  1:160064/1‑251 (MQ=255)                                                                                                                 aCATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGc                                                                                                                                >  1:59395/1‑251 (MQ=255)                                                                                                                   aggtacTGCAAGGCATTAGGTTGTTGGGGCAGTGCCCGGATAGAATCAACGCTGCGCTGATTTGCCGTGGCGAGAAGATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTAACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGACGGCTTGCGGCAAGTCGCATTCAGGATGATCACATGGTGCTg                                                                                                                              >  2:236889/6‑251 (MQ=255)                                                                                                                      tCCTGCATGGCATTAGTTTGGTTGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGGATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTCCCGTCGATATTGCTGCGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGATGATCACATGGTGCTGATg                                                                                                                           >  2:3377/1‑251 (MQ=255)                                                                                                                       ccTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCAACCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATgg                                                                                                                          >  1:61806/1‑251 (MQ=255)                                                                                                                                                                                        gCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACATCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGGACTTGGACGCAACGGTTCCGacta                                                          >  2:202399/1‑250 (MQ=255)                                                                                                                                                                                                                       cGTATTAGCAGCGCGCGGTCACTACGTTACCGTTCTCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGCGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTActccgcg                                                   >  2:127266/1‑222 (MQ=255)                                                                                                                                                                                                                                            caACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGAATTgg      >  1:9183/1‑250 (MQ=255)                                                                                                                                                                                                                                                gTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTACGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGCGATTTggacgg  >  1:250914/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     GCGATGCCGAACGTATTTTTGCCGAACTTTTGACGGGACTCGCCGCCGCCCAGCCGGGGTTCCCGCTGGCGCAATTGAAAACTTTCGTCGATCAGGAATTTGCCCAAATAAAACATGTCCTGCATGGCATTAGTTTGTTGGGGCAGTGCCCGGATAGCATCAACGCTGCGCTGATTTGCCGTGGCGAGAAAATGTCGATCGCCATTATGGCCGGCGTATTAGAAGCGCGCGGTCACAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGG  >  NC_000913/530‑1018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑