Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,253,002	C→T	intergenic (+17/+83)	dhaR → / ← ycgV	DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,253,002	0	C	T	100.0%	49.3 / NA	18	intergenic (+17/+83)	dhaR/ycgV	DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/9);  total (9/9)

CGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTGTACTTCTCCCGGG  >  NC_000913/1252775‑1253245                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    cGAGCAGGCGACATATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTAt                                                                                                                                                                                                                              <  2:84943/251‑1 (MQ=255)   aGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGTCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTAttt                                                                                                                                                                                                                            <  2:168128/251‑1 (MQ=255)      aGGCGACAGAGGATGTCAGCTCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGTTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGAGCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCAt                                                                                                                                                                                                                         <  2:121506/251‑1 (MQ=255)         cGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTcc                                                                                                                                                                                                                      <  2:233233/251‑1 (MQ=255)                                                              gTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACtt                                                                                                                                                                 <  1:181575/251‑1 (MQ=255)                                                                            aaTTATTAACGCAGCCCAGGTCACAGGCGGTTGCATTCAGGAAATGTCGGCTTTACTTGGGGTCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGGATTGATGCAGGGCAGGTTAAGCGCCGGGTATGAAAGACAGAAAAGATTTTTTATACATCCGAGTGATCTGTATTTCATTCCTGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGGTTGTCTTTCCCGCACTTTAAAGCTGAAGCg                                                                                                                                                    >  2:242606/1‑250 (MQ=255)                                                                                  taACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAACTGAAGCGATAtcct                                                                                                                                             >  1:50692/1‑251 (MQ=255)                                                                                       cAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTTGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGGGATCTGTAGTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGtt                                                                                                                                        <  2:240249/251‑1 (MQ=255)                                                                                            cAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGCTCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACAAGGCCTTGATGCAGGGCAGTTTAAGCGCCCGGTATGGAAGGCAGGAACGATTTTTGGTACATCAGAGGGATCTGTATTTTATTCCGGCGCACGCTAACAATTTTTAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTTAAGCGGTATTCTCCGTTGACtt                                                                                                                                   >  1:116693/1‑251 (MQ=255)                                                                                             aGGTCACAGGCGGTCGCTTTCAGGAAATGTCCTCCTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGAAACATGGAATTGGTGGAGGGCCGTTTAAACGCCGGGTCTGAAAGAAAGAAACGATTTTTTATACATCAGAGTGGACTGTATTTCATTCCGGCGCACGCTAACAATTTTTAGGATCGTTTAAGGGCTTTACTATCCCGCACCTAAAAGCTGAAGAGATATCCTCCGTTTACttt                                                                                                                                  >  2:39155/1‑250 (MQ=255)                                                                                                         gTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCaa                                                                                                                       >  1:170189/1‑250 (MQ=255)                                                                                                                         gTCGGCTTTACTTGGGATCGGCCGCACTACGCTGCGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAGGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCgtg                                                                                                      >  1:8722/1‑251 (MQ=255)                                                                                                                                                                      gAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGc                                                          >  1:56999/1‑250 (MQ=255)                                                                                                                                                                                               cAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTGGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGc                                <  2:50692/251‑1 (MQ=255)                                                                                                                                                                                               cAGTTTAAGCCCCGGTTATGAAAGACAGAAACGATTTTTGATTCATCAGATTTATCTTGATTTCTTTCCGTCGCACGCTAACAATTTCCAGCATCGTTTAAGGGCTTGTCTAACCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTAACTGGCTTCGGATCAAAGAGGTTACCCTGCGTGTAATCCGCTGCGAGATAGATTGTGTGCTGTTGGTTATCCGGGGCACTTACTCCCACACCGTTATTCCAGc                                <  2:9108/251‑1 (MQ=255)                                                                                                                                                                                                              ggaTGAAAGACAGACACGAATTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGTAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTCTACTGTGCACTGCCTCCCACGCCTTTATTCCAGCCATTACCTTTGaacc                 >  2:147809/3‑249 (MQ=255)                                                                                                                                                                                                                    aaGACAGAAACGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAAGTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTGCCCTGCGTGTAATCCGCTTCTAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTGTACt           <  1:21698/251‑1 (MQ=255)                                                                                                                                                                                                                             aCGATTTTTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACCATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTCAAAGCTGACGCGATATCCTCCGTTGCCTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACTCCGTTATTCCAGCCATTACCTTTGAAACTGTACTTCTCCCggg  >  1:269346/1‑251 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    CGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCGGTCGCATTCAGGAAATGTCGGCTTTACTTGGGATCGGCCGCACTACGCTGTGGCGGAAAATGAAGCAACATGGCATTGATGCAGGGCAGTTTAAGCGCCGGGTATGAAAGACAGAAACGATTTCTGATACATCAGAGTGATCTGTATTTCATTCCGGCGCACGCTAACAATTTTCAGCATCGTTTAAGGGCTTGTCTATCCCGCACTTAAAAGCTGAAGCGATATCCTCCGTTGACTTGCTTCTGATCAAAGAGGTTACCCTGCGTGTAATCCGCTTCGAGATAGAATGTGTGCTGTTTGTTATACTGTGCACTGACTCCCACGCCGTTATTCCAGCCATTACCTTTGAAACTGTACTTCTCCCGGG  >  NC_000913/1252775‑1253245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑