Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,252,663	C→T	S533F (TCT→TTT)	dhaR →	DNA‑binding transcriptional dual regulator DhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,252,663	0	C	T	100.0%	46.8 / NA	16	S533F (TCT→TTT)	dhaR	DNA‑binding transcriptional dual regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/4);  total (12/4)

ATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCG  >  NC_000913/1252431‑1252878                                                                                                                                                                                                                                         |                                                                                                                                                                                                                        aTTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGACAGGCa                                                                                                                                                                                                        >  2:234572/1‑250 (MQ=255)       gTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGAttt                                                                                                                                                                                                 >  1:168128/1‑251 (MQ=255)            aGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAAc                                                                                                                                                                                            >  1:265828/1‑251 (MQ=255)             gTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGCACt                                                                                                                                                                                           >  1:174906/1‑251 (MQ=255)                                 ggACCTCACAATGCTGGAGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCCGCATTCCGGCGCCGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGCTGCCCTCGCTCGCCTGGGTTTTTGTGCATGGCCAGGAAACGATTTTGAACTTTACAGCGTCATCGAAAATc                                                                                                                                                                       >  2:21698/1‑251 (MQ=255)                                                   ggAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGAt                                                                                                                                                      >  1:84943/1‑250 (MQ=255)                                                   ggAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAAATACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCCTGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGAt                                                                                                                                                      >  2:10733/1‑250 (MQ=255)                                                                tgtAGTCGCCAGCTGTATTAGCCTCTGCATGCATGTGAAATTACCACCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTGTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTc                                                                                                                                         <  1:24403/248‑1 (MQ=255)                                                                                           catgcatTTGCAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTAAGGCGCTGGTGAATAACAAATTAAGCAGACTTGAAAAACGCTTCTCTAAGCGATTGAAAATTGATAACCATGCCCTAGCTCGCCTGGTTTTTTGTGCATGGACAGGCCACGATTTTGAACTTTACCGCGACATCGAGAATCTTGCTCTGAGTGGTGATAACGGGGGCATTCGAGTCAATGATTTGCCGCAACATCTggt                                                                                                             >  1:153955/1‑249 (MQ=255)                                                                                           aatgcatTTGAAAATACCATCCCGCATCTGCGTATGCGGCGTGGCCGCATTCCGGCGCTGGAGAATAACAAATTACGCAGTCTTGAAAAACGCATCTCTACGCGGCTGAAAATGGATGACGATGACCTAGATCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGGCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCCTACGCGTCGGTGAGTTACCGGAACATCTGtt                                                                                                             >  1:153949/2‑251 (MQ=255)                                                                                            atgcctTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAACTTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGttt                                                                                                            >  1:240249/1‑251 (MQ=255)                                                                                                          ccccacccGCCTCTGCTTATGCGGCGTGGCATCTTTCCGGCGCGGTTGAATAACAAATTACGCATGCTTGAAAAACGCTTCTCTACGCGGCTGAAGATTGATGACGATGCCCGCGCTCGCCTGGGTTTTTGTTCATGGCCAAGGAACGATTTTGAGGTTTGCAGTTTCAGCTAGAATCTTTCTGTGAGTAGTGATAACGGGCGGATTCGCGTCAGCGATGTTCCGGAACATcgatctaccgggcgcgcgac                                                                                              <  2:112870/246‑20 (MQ=255)                                                                                                                                                      ggTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTg                                                  <  1:2809/251‑1 (MQ=255)                                                                                                                                                                      cGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAaga                                  <  1:14653/251‑1 (MQ=255)                                                                                                                                                                        cAGTCTTGAAAAACGATTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAgg                                >  2:181575/1‑251 (MQ=255)                                                                                                                                                                                                      gAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTTTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCg  >  1:9108/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                        ATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCATGCATTTGAAATTACCATCCCGCCTCTGCGTATGCGGCGTGGCAGCATTCCGGCGCTGGTGAATAACAAATTACGCAGTCTTGAAAAACGCTTCTCTACGCGGCTGAAAATTGATGACGATGCCCTCGCTCGCCTGGTTTCTTGTGCATGGCCAGGCAACGATTTTGAACTTTACAGCGTCATCGAGAATCTTGCTCTGAGTAGTGATAACGGGCGCATTCGCGTCAGTGATTTGCCGGAACATCTGTTTACCGAGCAGGCGACAGATGATGTCAGCGCCACCCGCCTTTCCACCAGTCTGTCATTTGCGGAAGTTGAAAAAGAGGCAATTATTAACGCAGCCCAGGTCACAGGCG  >  NC_000913/1252431‑1252878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑