Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,252,059	C→T	P332S (CCG→TCG)	dhaR →	DNA‑binding transcriptional dual regulator DhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,252,059	0	C	T	100.0%	37.0 / NA	14	P332S (CCG→TCG)	dhaR	DNA‑binding transcriptional dual regulator DhaR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/7);  total (7/7)

CAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAGCACCAGTTTATTGATGCGGTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCA‑TATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTCCTTATATCGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGT  >  NC_000913/1251847‑1252289                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       cAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAGCACCAGTTTATTGATGCGGTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCA‑TATGCCACAGGACGATTCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAggcg                                                                                                                                                                                                  >  1:31566/1‑251 (MQ=255) aaaTAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAGCACCAGTTTATTGATGCGGTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATCCCTTCGCTCA‑TATGCCACAGGACGATTCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAggcg                                                                                                                                                                                                  <  1:15035/250‑1 (MQ=255)  aaTAAAACAGGCACATCCGCTAAAACACGTAAAAGCAACCTTTGAAAGCCAGCACCAGTTTATTGATGCGGTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCA‑TAGGCCACAGGACGAGTCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAggcgg                                                                                                                                                                                                 <  2:218592/251‑1 (MQ=255)                                                               atagATGCGGGGATAACCCTTAAACCGAAAAAAGATACGCAGGGACCGATCTTTATTGTTTGGCTCCATCCGGTGGAACAAATGCGGCAGTGGATGACAAGATAATTAGGAAAAGTCAGCCATACCTCCGCTCT‑GATGCCACAGGACAATTCGCAACGCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTACCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAg                                                                                                                                    <  1:136327/248‑1 (MQ=255)                                                                         tGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCA‑TATGCCACAGGACGATTCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAAtt                                                                                                                          >  2:79009/1‑251 (MQ=255)                                                                          ataaaCCTTTAAACCGATAATAGAAACGAAGGGACCCGGCTTTATTTTGTTGCTCCATCCTGTGAAACATATGCGGCAGTTGGTGACCAGTAAATTAGGAAAAGTCACCCATCCCTTCGCTCA‑TATGCCCCAGGACGATTCGAAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTc                                                                                                                         <  2:219575/248‑1 (MQ=255)                                                                                        cGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCA‑TATGCCACAGGACGATTCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAg                                                                                                                                    <  1:125665/226‑1 (MQ=255)                                                                                        cGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCA‑TATGCCACAGGACGATTCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAg                                                                                                                                    >  2:125665/1‑226 (MQ=255)                                                                                          ataataGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCA‑TATGCCACAGGACGATTCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAagcgagcg                                                                                                         >  1:38278/1‑251 (MQ=255)                                                                                                                               tCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCA‑TATGCCACAGGACGATTCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTCCTTATATCGCCGTCAATTGTGAGtt                                                                     >  1:81811/1‑250 (MQ=255)                                                                                                                                                    ggCAGTTGATGCCCAGTAAATTAGGAAAAGTCAGCCTTCCCTTCG‑TCAATATGCCACAGGACGATTCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTCCTTATATCGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCg                                               <  1:257782/251‑1 (MQ=255)                                                                                                                                                            atgaCCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCA‑TATACCACAGGACGATTCGCAAACCCGACGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTCCTTATATCGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAAtt                                       >  1:35155/1‑251 (MQ=255)                                                                                                                                                                                   cAGCCATACCTTCGCTCA‑TATGCCACAGGACGATTCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTCCTTATATCGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGATCGCACGGAc                 >  2:36409/1‑250 (MQ=255)                                                                                                                                                                                                  ccA‑TATGCCACAGGCCGATTCGCCAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTTCTAAGTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTTCTTATATCGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGt  <  1:225479/249‑1 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       CAATAAAACAGGCACATCCGCTCAAACACGTAGAAGCAACCTTTGAAAGCCAGCACCAGTTTATTGATGCGGTGATAACCCTTAAACCGATAATAGAAACGCAGGGAACCAGCTTTATTTTGTTGCTCCATCCTGTGGAACAGATGCGGCAGTTGATGACCAGTCAATTAGGAAAAGTCAGCCATACCTTCGCTCA‑TATGCCACAGGACGATCCGCAAACCCGCCGCTTGATTCATTTTGGTCGCCAGGCGGCGCGCAGTAGCTTTCCTGTCCTGCTTTGTGGAGAAGAGGGCGTGGGCAAGGCACTGCTAAGTCAGGCAATTCATAATGAAAGCGAGCGTGCTGCAGGTCCTTATATCGCCGTCAATTGTGAGTTATATGGTGATGCTGCGCTGGCGGAAGAATTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGT  >  NC_000913/1251847‑1252289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑