Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,201,550	C→A	T18P (ACC→CCA)	ymfI →	uncharacterized protein YmfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,201,550	0	C	A	87.5%	19.7 / NA	8	T18T (ACC→ACA)	ymfI	uncharacterized protein YmfI
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (1/6);  total (2/6)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTATCCACAGAAGGTCGATTGTCAGCCTCAACCTTAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGACCAAAA‑AATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTCTGCTTCCTTAGTTGGTAACGATT  >  NC_000913/1201315‑1201791                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   ggtATCCACAGAAGGTCGATTGTCAGCCTCATCCTGAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGGTATGTGCCTGTCTTTTCACCACTTCAGGCGCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTGAGTATATTTGAAGACACAGCCGAACAGCCAAAAA‑AATTTtaagaa                                                                                                                                                                                                                                    <  2:22225/251‑1 (MQ=255)                        cAGCCTCAACCTTAACCCGCGTGCGCTGCGCCGCATTCACCCCAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGAGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAA‑AATTTGAAGAATTAATGCTTAACTTTCTGTAcc                                                                                                                                                                                                              >  2:255718/1‑250 (MQ=255)                                                                                                           aTGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAA‑AATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCcaa                                                                                                                           <  1:65248/250‑1 (MQ=255)                                                                                                                                  cAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAA‑AATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGTTATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCa                                                                                                   <  2:14786/251‑1 (MQ=255)                                                                                                                                          gtggtATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAA‑AAGTTGAAGAAGTAATGCTTAACGTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATACCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTaa                                                                                           <  2:96774/251‑1 (MQ=255)                                                                                                                                                    ggAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAA‑AATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCgg                                                                                  <  2:21257/250‑1 (MQ=255)                                                                                                                                                                                                                    aTTTGAAGACACAGCCGAACAGCCAAAAA‑AATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTCTGCTTcc                  <  1:255718/250‑1 (MQ=255)                                                                                                                                                                                                                                   cGAACAG‑CCAAAACAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTCTGCTTCCTTAGTTGGTAACGAtt  >  2:129942/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   GGTATCCACAGAAGGTCGATTGTCAGCCTCAACCTTAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGACCAAAA‑AATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTCTGCTTCCTTAGTTGGTAACGATT  >  NC_000913/1201315‑1201791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑