| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,201,548 | A→C | T18P (ACC→CCA) | ymfI → | uncharacterized protein YmfI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,201,548 | 0 | A | C | 87.5% | 13.0 / ‑3.1 | 8 | coding (52/342 nt) | ymfI | uncharacterized protein YmfI |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (1/6); minor base . (1/0); total (2/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01 | |||||||||||
GGTATCCACAGAAGGTCGATTGTCAGCCTCAACCTTAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGACCAAAA‑AATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTCTGCTTCCTTAGTTGGTAACGATT > NC_000913/1201315‑1201791 | ggtATCCACAGAAGGTCGATTGTCAGCCTCATCCTGAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGGTATGTGCCTGTCTTTTCACCACTTCAGGCGCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTGAGTATATTTGAAGACACAGCCGAACAGCCAAAAA‑AATTTtaagaa < 2:22225/251‑1 (MQ=255) cAGCCTCAACCTTAACCCGCGTGCGCTGCGCCGCATTCACCCCAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGAGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAA‑AATTTGAAGAATTAATGCTTAACTTTCTGTAcc > 2:255718/1‑250 (MQ=255) aTGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAA‑AATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCcaa < 1:65248/250‑1 (MQ=255) cAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAA‑AATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGTTATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCa < 2:14786/251‑1 (MQ=255) gtggtATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAA‑AAGTTGAAGAAGTAATGCTTAACGTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATACCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTaa < 2:96774/251‑1 (MQ=255) ggAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGCCAAAAA‑AATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCgg < 2:21257/250‑1 (MQ=255) aTTTGAAGACACAGCCGAACAGCCAAAAA‑AATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTCTGCTTcc < 1:255718/250‑1 (MQ=255) cGAACAG‑CCAAAACAATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTCTGCTTCCTTAGTTGGTAACGAtt > 2:129942/1‑251 (MQ=255) | GGTATCCACAGAAGGTCGATTGTCAGCCTCAACCTTAACCCGCGTGCGCTGAGCCGCATTCACCACAACGCTAAGGATTCTCTCTGGTTGAAAATACTTAGCTGTTATGTGCCTGTCTTTTCACCACTTCAGGCTCGGTGGTATGCTGGAGTTCTCACACAGCCAGCAAGCAAGGAAACTTAATGAACCAGTTTTATGTTCACGTTCGTCTATTTGAAGACACAGCCGAACAGACCAAAA‑AATTTGAAGAATTAATGCTTAACTTTCTGTACCAGAAAACAGTTAAAGAGTCTGACGATAGCTGCTGCAGACTGATTCCAGAGGGATATATCCTCAAAAGTACAATGAACTGCCAACAAATCCTTGATCAAACATTTTCAATTGCTAACAGTGCCGGTGTTGACGCAAATATATTTGTCTGTAAATTTGAACAAAGCGCATGCTTACTTCCGTCTGCTTCCTTAGTTGGTAACGATT > NC_000913/1201315‑1201791 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |