Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,760	T→C	A808A (GCT→GCC)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,760	0	T	C	100.0%	75.7 / NA	25	A808A (GCT→GCC)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/10);  total (15/10)

GCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTACTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCTGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCT  >  NC_000913/2529‑3005                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       gcgcgTGGCGAAGGGCCGTGATGAAGGAAAAGGTTTGCGCTATGTTGGCAATATTGATGAAGAGGGTTCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCctct                                                                                                                                                                                                                                     <  2:160886/251‑1 (MQ=255)    cgTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCAt                                                                                                                                                                                                                                  <  1:263224/251‑1 (MQ=255)           aaGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGtt                                                                                                                                                                                                                           <  2:3795/251‑1 (MQ=255)                   tgatgaAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCt                                                                                                                                                                                                                   >  2:7167/1‑251 (MQ=255)                          ggataaGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGACTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAACTGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGACCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATgg                                                                                                                                                                                                            >  2:61394/5‑251 (MQ=255)                                         tATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGCAGTTAGGAGTCTGACATGGTTAAAGTTTATGccc                                                                                                                                                                                             >  1:17262/1‑251 (MQ=255)                                          aTGTTGGCAATATTGATGTAGATGGAGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTATCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTCTGcccc                                                                                                                                                                                            >  2:257595/1‑251 (MQ=255)                                                           gaAGATGGAGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAACATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTCAAGTTTATGCCCCGGCTTCCAGTGCCAat                                                                                                                                                                            >  2:129307/1‑250 (MQ=255)                                                             aGATGGTGCCTGCCGCGTGAAGATTGCCGCAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTCTCAGCCGCTGCCGTTGCTGCTGCGAGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATg                                                                                                                                                                         >  1:244478/1‑251 (MQ=255)                                                                                       ccGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGCGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGGGCCAAAATGAGCGTCGGGTTTGATGTGCTCgggcc                                                                                                                                               >  1:1421/1‑249 (MQ=255)                                                                                           aGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGgcggcg                                                                                                                                           <  1:257595/251‑1 (MQ=255)                                                                                                         aTCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGATAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTgg                                                                                                                            >  2:2401/1‑250 (MQ=255)                                                                                                                             aaTGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGGTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGAATTGCTCGgagttg                                                                                                        >  2:32890/1‑248 (MQ=255)                                                                                                                                  cGAAAACGCCCTGTCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGGGCGGGCAATGACGTTACAGCTGCCTGTGTCTTTGCCGATCTGCTACGTACCCTCGCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTGCAGTGCCAATATGAGCGTCGGGTTTGATGGGCTCGTGGCGGCGGT‑GACACCTGTTGATGGTGCATTGTTCGGTGATGTAGTc                                                                                                   <  2:176925/251‑1 (MQ=255)                                                                                                                                   gAAAACGCCCTGGCCTTTTATAGCCAATATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCACTATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTTGA‑ATCTGTTGGTGGTGCATTgtatggagttggaagc                                                                                                   >  2:211390/1‑235 (MQ=255)                                                                                                                                            cTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAgg                                                                                         >  1:1165/1‑251 (MQ=255)                                                                                                                                                        aGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACAt                                                                             <  1:2401/251‑1 (MQ=255)                                                                                                                                                                              cgtttgtgctgcgcggtttaggTGCTGGCAAGGACGTTACAGCTTCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATTTGCTCGGGGCTGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCTGCTGAGCCATTCTGTCTCCCCACCCTCGGAcc                                                       <  2:149474/231‑2 (MQ=255)                                                                                                                                                                                                     gCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAg                                <  1:61394/251‑1 (MQ=255)                                                                                                                                                                                                              gACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑Ga                                                                                                                                      >  1:49815/1‑140 (MQ=255)                                                                                                                                                                                                              gACGTTACAGCTGCCGGTGGCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGggcgttggtga                                                                                                                                       <  2:49815/140‑8 (MQ=255)                                                                                                                                                                                                                        cTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGGTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTGGCCCATAACCCGCCGGCAGAAACACGGGAAAATATCGt             >  2:190412/1‑251 (MQ=255)                                                                                                                                                                                                                               tgtCTTTGCCGATCTGCTAAGTACCCTCTCATGGAAGTTAGGAGTCTGACTTGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCAAGGGGAAATATCGtttctc        >  1:239931/1‑246 (MQ=255)                                                                                                                                                                                                                                   tttGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGGTAAGCTGCCGTCAGAACCACGGGAAAAGATCGTTTTTCATTGCt  >  2:151071/1‑251 (MQ=255)                                                                                                                                                                                                                                    ttGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGc   <  2:1165/249‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       GCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTACTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCTGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACATTCAGTCTCAACAACCTCGGACGCTTTGCCGATAAGCTGCCGTCAGAACCACGGGAAAATATCGTTTATCAGTGCT  >  NC_000913/2529‑3005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑