Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,176,602	C→T	P392P (CCC→CCT)	lolC →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,176,602	0	C	T	100.0%	89.4 / NA	29	P392P (CCC→CCT)	lolC	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/20);  total (9/20)

TATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAG  >  NC_000913/1176368‑1176839                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              tATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGt                                                                                                                                                                                                                                <  1:68184/250‑1 (MQ=255)          aGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAg                                                                                                                                                                                                                      <  1:261994/251‑1 (MQ=255)             ggCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATa                                                                                                                                                                                                                        >  1:17515/1‑246 (MQ=255)                cAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTg                                                                                                                                                                                                                >  2:213115/1‑251 (MQ=255)                                   gggATCCTGGGCGCGGCGCTTGGCGCACTGCTTGCCATGCAGTTAAATAATCTGATGCCGATAAGCGGCGTCCTTCTTGATGGCGCGGCGCTGCCGGTGGCTATCGACCCTTTTCAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCAGTGCGCTCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTATTGCAATGCGACATCCtgt                                                                                                                                                                                             <  2:4972/249‑1 (MQ=255)                                                       tGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAAGCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAgg                                                                                                                                                                          <  1:93543/250‑1 (MQ=255)                                                          cgcCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAgg                                                                                                                                                                      >  1:87121/1‑250 (MQ=255)                                                            cccTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCGGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAgtgt                                                                                                                                                                    <  2:26789/251‑1 (MQ=255)                                                                      cagccagTTAAATAATCTGATGCCGATAGTCGGCGTCCTGCTTGATGGCGCGGCGCTGTCGGAGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCCCCACTCAACCTCCTGAGGCTTTACGTTATGAATACGATCCTGCTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGCGTGCAAACCg                                                                                                                                                            >  2:129225/1‑249 (MQ=255)                                                                                            ccGATAATCGGGGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCa                                                                                                                                    <  1:93743/251‑1 (MQ=255)                                                                                             cGATAATCGGCGTCCTGCTGGAGGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAg                                                                                                                                   <  1:228334/251‑1 (MQ=255)                                                                                                       cGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa                                                                                                                          >  2:247190/1‑250 (MQ=255)                                                                                                              cTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGCCATTCATATTGCGCTGGTGGCGATGGCTATCGAGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGCAAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGGGCTGCACACTGTCAGTTTCAGCGTCGGCGAAGGTGac                                                                                                                   >  1:20214/1‑249 (MQ=255)                                                                                                                            gcTGCCGGGGGCTACCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGAtcgtc                                                                                                    <  2:17515/251‑1 (MQ=255)                                                                                                                               gCCGGTGGCTATCGAACCTTTACAGGTCATTGTTTTTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTTTACGCTTTCCCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGAtcgtcg                                                                                                   <  1:152863/249‑1 (MQ=255)                                                                                                                                 cGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCACTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAg                                                                                               <  1:220091/251‑1 (MQ=255)                                                                                                                                                  ttACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTaaa                                                                               >  1:206925/1‑250 (MQ=255)                                                                                                                                                       ggTCATTGTTATTGCGCTGGGGGGGATGGCTATCGCGCTGCTGTCTCCGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGCTTCCGGTAAAAGTAcc                                                                         <  1:202251/251‑1 (MQ=255)                                                                                                                                                              gTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTtgctg                                                                   <  1:128652/250‑1 (MQ=255)                                                                                                                                                               ttATTGCGATGGTGGCGCTGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTtgctgc                                                                  >  2:249764/1‑250 (MQ=255)                                                                                                                                                                tATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCCCTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTtg                                                                      >  2:235041/1‑245 (MQ=255)                                                                                                                                                                tATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCGGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTtg                                                                      <  1:235041/245‑1 (MQ=255)                                                                                                                                                                              cgagggcgaTCGCGCTGCTGTCTACGCTTTCCCCCTCAGGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCTCCTGCTGGGCGGGCt                                                  <  2:22760/243‑1 (MQ=255)                                                                                                                                                                                   gCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATa                                             <  2:106417/251‑1 (MQ=255)                                                                                                                                                                                    gtATCGCGCTGTTGTTTACGCTTTACCCTTCAGGGCGCGCGGCCGCCACTCAACCTGCTGGGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATAc                                            <  2:39439/250‑1 (MQ=255)                                                                                                                                                                                                                 tCATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTaa                 <  1:213115/249‑1 (MQ=255)                                                                                                                                                                                                                  cATGGCGCGCTGCCGCCACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATgg              <  2:206925/251‑1 (MQ=255)                                                                                                                                                                                                                              ccgccACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAg  <  1:247190/251‑1 (MQ=255)                                                                                                                                                                                                                              ccgccACTCAACCTGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAg  <  1:129225/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              TATGGTGCAAGGGGCCAGCGCCGGGATTATCGGTGCGATCCTCGGAGCGGCGCTTGGCGCCCTGCTTGCCAGCCAGTTAAATAATCTGATGCCGATAATCGGCGTCCTGCTTGATGGCGCGGCGCTGCCGGTGGCTATCGAACCTTTACAGGTCATTGTTATTGCGCTGGTGGCGATGGCTATCGCGCTGCTGTCTACGCTTTACCCTTCATGGCGCGCTGCCGCCACTCAACCCGCTGAGGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAG  >  NC_000913/1176368‑1176839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑