Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,147,792	T→A	S58T (TCG→ACG)	plsX →	putative phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,147,792	0	T	A	100.0%	37.1 / NA	15	S58T (TCG→ACG)	plsX	putative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (8/7);  total (8/7)

TGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCA  >  NC_000913/1147550‑1148025                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                          tGCGTGATGAAGCTTAGTAAGGATTTTCCCCAGGCAACTGGGGAACGACCAAACCGGGCGGCGACGCTACCTTGACCCGTCTAACACTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTTAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTg                                                                                                                                                                                                                                   >  2:54319/1‑251 (MQ=255)                         tttCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGGCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGGCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGcagtcagt                                                                                                                                                                                                            <  2:179673/250‑1 (MQ=255)                                         gggAAAGACCAAACCGGGCGGCGACGATACCTTGACACGGCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTGTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTTATGccc                                                                                                                                                                                           <  2:103924/251‑1 (MQ=255)                                                  cAAACCGGGCGGGGGCGATGCCTTGAAACGTCTAACCCTGGCGTTAGAAGTCATGGGAAGGGATTGTGGCCCGTCCCTGACCATGCCTGCAACATTGCAGGCCCTGAATTCTAATTCGCAACTCCCTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCAGTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGc                                                                                                                                                                                  <  1:2950/251‑1 (MQ=255)                                                  cAAACCAGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGATAGATGTCATGGGAGGGGATTTTGGCCATTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTAAGTTATCGCCAGTGATGCCAGGCCTTCGc                                                                                                                                                                                  >  2:149556/1‑251 (MQ=255)                                                                                     cccTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATgcgc                                                                                                                                               >  1:131897/1‑251 (MQ=255)                                                                                              tggatgacgagggAGGGGCTTTTGGATCTTCCGAGACAGTGCCTGGAGCATTGCAGGCACTGGATTATAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGACATCACGCCATTACTTGCTAAAGCTGCCTTTGAACAACGAAAGCGTCCGCAGATTATTCCTGCGCAGGCAGTTATCGCCAGTGATGCCCGGCCTTAGCCAGCTATCCGCGCCAGTCGTGGGAGGTCAAGGCGCGTGGcccgg                                                                                                                                      >  2:54479/11‑249 (MQ=255)                                                                                                   gTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCt                                                                                                                                 >  1:123378/1‑251 (MQ=255)                                                                                                   gTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCt                                                                                                                                 >  1:29515/1‑251 (MQ=255)                                                                                                                       cccTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAgcgc                                                                                                             >  1:140771/1‑251 (MQ=255)                                                                                                                                 aCAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTgtgt                                                                                                   <  2:194060/251‑1 (MQ=255)                                                                                                                                                               ccTAAGTCGCAACTTACTCTGTTTTTAGCCTGCAATTCCAACGCCATCACGCCATGTCTTGCTAAAGCTGACTGTGAACAACGTACGCGTCTGCAGATTATTCCTGTGCAGTCAGTTATCGCCAGTGATGCACGGTCTTCGCAAGCTATCCGCGCAAGTCGTTGTAGTGCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTAGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATggg                                                                     <  2:25052/250‑1 (MQ=255)                                                                                                                                                                                                  taccgacggactCACGCGATTAATCGCTAAAGCTGACTTTTTACCACGTACGCATTTGCAGCTTATTTCTGGGCAGTCAGTTATCGCCAGTGAGGGCCGCCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCCATGCGCGTGCCCCTGGAGCTGGTGAAAGACGCCCGAGCGCACGGCTGTGTACGTGCCGGTAATACCGGGGAGCTGATGGGGCTGACAAAATTATTCCTCACGACCCTGTAggggc                                  >  2:90632/12‑250 (MQ=255)                                                                                                                                                                                                           cATCCCGCCATTGCTTGCGAAAGCGGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCGAGCCGCGCCTGTCGGGGGAGTTCAAGGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATGCCGGGGCGCTGATGGGGCTGGGAAAATTATTACTCAAGCCCCTTGAGGGGATTGAGCGTc                         <  2:123378/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaCTTTGAACACCGTACGCTTCTGCAGATTATTCCTGCGCCGTCAGTTATCGCCAGTGAGGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCTTGGGAGTTCAATGCTCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACca  <  2:61560/248‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                          TGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCA  >  NC_000913/1147550‑1148025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑