Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,146,384	A→G	V71A (GTA→GCA)	yceF ←	m(7)GTP pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,146,384	0	A	G	100.0%	62.1 / NA	21	V71A (GTA→GCA)	yceF	m(7)GTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/9);  total (12/9)

CGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATACCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTCCATCAGATATAGCG  >  NC_000913/1146152‑1146618                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           cgcgCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTAGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCaata                                                                                                                                                                                                                           >  1:183224/1‑250 (MQ=255)                                                    gCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGATTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCAGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGca                                                                                                                                                                        >  2:179819/1‑249 (MQ=255)                                                                           cAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAACCAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCggg                                                                                                                                                >  2:113665/1‑250 (MQ=255)                                                                              aaGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGACAGTCGAAGCACCAACTGTCGCGGTGAt                                                                                                                                             >  1:48971/1‑251 (MQ=255)                                                                                ggCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATtc                                                                                                                                           <  1:198058/251‑1 (MQ=255)                                                                                  cTCCACTTCTGTTTACAGCTGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGACGCTGGCTTTGCGTACTTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATACCGTGACGCCAGATATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATtcgt                                                                                                                                         >  2:39054/1‑251 (MQ=255)                                                                                    ccACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACGGTCGCGGTGATtcgtcg                                                                                                                                       <  2:18852/251‑1 (MQ=255)                                                                                            gTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCAAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTgg                                                                                                                               >  2:166357/1‑251 (MQ=255)                                                                                              ttGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCATGATAGAATGTGACGATATTGCCGCTTGCTTTGCGTAATTGCAGACGGTCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGATCACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTGTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTggg                                                                                                                              <  2:48971/250‑1 (MQ=255)                                                                                                   gATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTCCCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGTACCAATAATTAAATGa                                                                                                                                                                                                                  >  1:165107/1‑161 (MQ=255)                                                                                                   gATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCGGGTCTGAACCAATAATTAAATGa                                                                                                                                                                                                                  <  2:165107/161‑1 (MQ=255)                                                                                                                                agtccagtATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAaga                                                                                           <  1:113665/251‑1 (MQ=255)                                                                                                                                            acggTGACGCTATTGCCACTGGCTTTGCGTCATTGCAGCCGGGCATCTTCTTCCGTCAACGGTTTCCCGGTGATTTAGCCATCAAGCACACATGCCTGGTCTGAACCACTAATTAAATGATCCGGCTAACTTGACGCCAGAGCTTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGATGATTCGTCGCTGCGTGGGGGTTCGTCGACCTCTGGGGCTGCACATTCACACGCGATTTGCCGttt                                                                               >  2:121334/3‑251 (MQ=255)                                                                                                                                                      tatTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCaga                                                                        >  2:139153/1‑248 (MQ=255)                                                                                                                                                                    ttGCGTAATTGCAGACGGGCATTTTCTTCCGTTAAGGGTTTACCGGAGATTTCGCCATAAAGAACACATGCCTGGTCTGCACCAATAATTAAATGATCCGGATAACGTGACGACAGAGATTGCGCTTTTTATTGTGCCAGTCGAAGAACCACCTGTCGCGGAGATTCGTCGCTGCGTGGGGATTCGTCGCCCTCTGGTGCTGCACATTCAAAAGCGATTTGCAGTTTTTCCAGAAGCGCGCGACGCCATgg                                                       >  1:43536/1‑251 (MQ=255)                                                                                                                                                                          aaTTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAgg                                                  <  1:139153/250‑1 (MQ=255)                                                                                                                                                                                          tttCTTCAGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAATGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGAGGAGGCTAAAATCAGt                                 >  1:105048/1‑251 (MQ=255)                                                                                                                                                                                                ccGTTAACGGTTTACCGGTGATTTCGCCAGCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAgg                            <  2:161569/250‑1 (MQ=255)                                                                                                                                                                                                  gTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAAGAAGTTTAGGCat                         <  1:207060/251‑1 (MQ=255)                                                                                                                                                                                                   ttAAAGGTTTACCGGTGATTTAGCCATCAAGACCACATGCCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGCCGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGCGCTGCACATTCAACAGAGATTTGCAGTTTTTCCAGAAGAGCGCAACGCCATGGCGAGGTGGCGGCTCAAATAAGTTTAGGCat                         >  2:120006/1‑250 (MQ=255)                                                                                                                                                                                                                         gCCATCAAGAACACATGCCGGGTACGAACCAATAATTAAAGGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGGCAGTCGAAGCACCAAAAGTCGCGGTGATTCGCCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACAATCAAAAGAGATTTGCAGTTTTTCCATAAGAGCGCGACGCCATGTCGAGGTTGAGGCTAAAATAAGTTTAGGCATATTTTTTGCCATCAGATATAGCg  <  1:39054/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           CGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATACCTGGTCTGAACCAATAATTAAATGATCCGGATAACGTGACGCCAGAGATTGCGCTTTTTCTTGTGCCAGTCGAAGCACCAACTGTCGCGGTGATTCGTCGCTGCGTGGGGTTTCGTCGACCTCTGGTGCTGCACATTCAAAAGAGATTTGCAGTTTTTCCAGAAGAGCGCGACGCCATGGCGAGGTGGAGGCTAAAATAAGTTTAGGCATATTTTTTTCCATCAGATATAGCG  >  NC_000913/1146152‑1146618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑