Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,709	A→G	V791V (GTA→GTG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,709	0	A	G	100.0%	55.1 / NA	21	V791V (GTA→GTG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/8);  total (13/8)

CGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTACTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCTGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACAT  >  NC_000913/2466‑2930                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                               cgctgAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGGTCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGGGGATGGTAATGATCAGCTGTTCAAAGTGAAAAATGGCGAAAACGACCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGcgcg                                                                                                                                                                                                                         >  2:82174/5‑251 (MQ=255)   cggAGGGTGATGTTGCCGCTTTTATGGGGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAAGGGCGAAAACGCCCTGGCCTTTTAGAGCCACTATTATCAGCCGCGGCCGTTGGTGCTGCGCgg                                                                                                                                                                                                                        <  2:215709/248‑1 (MQ=255)                               gAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAACCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTg                                                                                                                                                                                           >  1:3795/1‑251 (MQ=255)                                                                gcgcgTGGCGAAGGGCCGTGATGAAGGAAAAGGTTTGCGCTATGTTGGCAATATTGATGAAGAGGGTTCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCctct                                                                                                                                                          <  2:160886/251‑1 (MQ=255)                                                                   cgTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCAt                                                                                                                                                       <  1:263224/251‑1 (MQ=255)                                                                          aaGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGtt                                                                                                                                                <  2:3795/251‑1 (MQ=255)                                                                                  tgatgaAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCt                                                                                                                                        >  2:7167/1‑251 (MQ=255)                                                                                         ggataaGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGACTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAACTGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGACCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATgg                                                                                                                                 >  2:61394/5‑251 (MQ=255)                                                                                                        tATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGCAGTTAGGAGTCTGACATGGTTAAAGTTTATGccc                                                                                                                  >  1:17262/1‑251 (MQ=255)                                                                                                         aTGTTGGCAATATTGATGTAGATGGAGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTATCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTCTGcccc                                                                                                                 >  2:257595/1‑251 (MQ=255)                                                                                                                          gaAGATGGAGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAACATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTCAAGTTTATGCCCCGGCTTCCAGTGCCAat                                                                                                 >  2:129307/1‑250 (MQ=255)                                                                                                                            aGATGGTGCCTGCCGCGTGAAGATTGCCGCAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTCTCAGCCGCTGCCGTTGCTGCTGCGAGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATg                                                                                              >  1:244478/1‑251 (MQ=255)                                                                                                                                                      ccGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGCGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGGGCCAAAATGAGCGTCGGGTTTGATGTGCTCgggcc                                                                    >  1:1421/1‑249 (MQ=255)                                                                                                                                                          aGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGgcggcg                                                                <  1:257595/251‑1 (MQ=255)                                                                                                                                                                    aaTGAGCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCGGGCCGTTTATAGCCACTATTATCAGCCGCTGCCGTTAGTGCTGCGCTGatat                                                                                                                                                                                                                    <  2:138839/93‑1 (MQ=255)                                                                                                                                                                        aTCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGATAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTgg                                                 >  2:2401/1‑250 (MQ=255)                                                                                                                                                                                            aaTGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGGTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGAATTGCTCGgagttg                             >  2:32890/1‑248 (MQ=255)                                                                                                                                                                                                 cGAAAACGCCCTGTCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGGGCGGGCAATGACGTTACAGCTGCCTGTGTCTTTGCCGATCTGCTACGTACCCTCGCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTGCAGTGCCAATATGAGCGTCGGGTTTGATGGGCTCGTGGCGGCGGT‑GACACCTGTTGATGGTGCATTGTTCGGTGATGTAGTc                        <  2:176925/251‑1 (MQ=255)                                                                                                                                                                                                  gAAAACGCCCTGGCCTTTTATAGCCAATATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCACTATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTTGA‑ATCTGTTGGTGGTGCATTgtatggagttggaagc                        >  2:211390/1‑235 (MQ=255)                                                                                                                                                                                                           cTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAgg              >  1:1165/1‑251 (MQ=255)                                                                                                                                                                                                                       aGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACAt  <  1:2401/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                               CGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTACTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCTGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGGCGGCAGAGACAT  >  NC_000913/2466‑2930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑