| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,138,533 | C→T | G52G (GGC→GGT) | flgK → | flagellar hook‑filament junction protein 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,138,533 | 0 | C | T | 91.7% | 24.4 / ‑6.2 | 12 | G52G (GGC→GGT) | flgK | flagellar hook‑filament junction protein 1 |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (5/6); total (6/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGCTGGGTTGGCAATGGTGTCTACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGA‑CAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGT > NC_000913/1138286‑1138739 | aGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGACAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGCTgg > 1:203229/1‑251 (MQ=255)aGTAGGAACATTGATAACCTGTTCTGAATAACTCAAGGCGGGCGGGGGGCTGCCAATAATACTCTGTAATTGAAGGCTTATAAGAAACCCCCATGCCCAGCTTGATTAATAACGCCAGGAGCGGACGGAGCGCGGCCCAGGCGGCTTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATGCCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTgg < 1:110374/251‑1 (MQ=255) gcggGTCGCTGCCGATAATCCTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCTACGTTTCTGGTGTGCAGCGTGAGTa < 1:254893/251‑1 (MQ=255) tGCCGCTAATACTCTGTAATTGACGGCTTATAAGGCACCTCCATGTCCAGCATGATTAATCACGCAATGAGCGTACTGAACGCGGCTCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATAACCGCCAAACCACTATTATGTCGCAGGCCAATCGCACGTTGGGCTCTGGCGGTTGGGTTGGCACAGGTGTCTACCTTTCTTGTGAGCTGCGGGTGTATGATGCggt > 2:261437/1‑249 (MQ=255) gAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCTACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATg < 2:205825/250‑1 (MQ=255) acggAACGCGGCCCAGGCGGCGTTAAATACGGAAAGTAATAATATCACCAGCTAAAGCGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCGGGCGGTTGGGTTGGCAATGGTGTCTACGTTTCTGGTGTGCAGCGTGAGTAGGATGCGTTTAGTTCCATCCAGTTACGTGCGGCGCAGACGCAAAGGAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGaaaa < 2:76467/248‑1 (MQ=255) aaCGCGGCCCACGCGGCGTTGAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGGGTCTATGTTTCTGGTGGGCAGCGTGTGGATGATGCGTTTCTTACCAACCCGTTACGGGCGGGGCCGACGCAAAGTAGAGGTCTGGCTGCCCGCTCTGAGCCGATGTCGCAAATCgg > 2:156997/1‑250 (MQ=255) ggCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCTACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGA‑CAata < 2:219029/251‑1 (MQ=255) cAGCGATAACTTTGCCGGATATACCCGCCAAACCACGATGATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCTACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAAGCGA‑CAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGc < 1:142997/251‑1 (MQ=255) ggATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCTACGTTTCTGGTGGGCAGCGTGAGGATGATGCGTTTATTACCAACCAGTTAAGGGCGGCGCAGACGCAAAGTAGGGGTCTGAATGCCCGCTATGAGCAGATGTCGCAAATCGA‑CAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGGTTTCTTCAcc > 1:105306/1‑249 (MQ=255) aaCCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGTGTCTACGGTTCTGGTGTGCAGCGTGCGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCCATGAGCAGATGTCGAAAATCGA‑CAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCCCCAGCCTGCAAACGCTgg > 1:27437/1‑251 (MQ=255) aCCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGTTGGGTTGGCAATGGGGTCTACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGAAGCAAAGTAGCGGTCTGACTGCCCGCTTTGAGCAGATGTCGAAAATCGAACAA‑ATGCTCTCCACCAGTACCTCCTCGCCGGCCACACCGATGCAGGATTTTTTCACCCACCTGCAAACGCTggg > 2:75753/1‑250 (MQ=255) | AGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATTAATAACGCCATGAGCGGACTGAACGCGGCCCAGGCGGCGTTAAATACGGCAAGTAATAATATCTCCAGCTATAACGTTGCCGGATATACCCGCCAAACCACTATTATGGCGCAGGCCAATAGCACGTTGGGCGCTGGCGGCTGGGTTGGCAATGGTGTCTACGTTTCTGGTGTGCAGCGTGAGTATGATGCGTTTATTACCAACCAGTTACGTGCGGCGCAGACGCAAAGTAGCGGTCTGACTGCCCGCTATGAGCAGATGTCGAAAATCGA‑CAATATGCTCTCCACCAGTACCTCTTCGCTGGCAACACAGATGCAGGATTTCTTCACCAGCCTGCAAACGCTGGT > NC_000913/1138286‑1138739 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |