Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,138,152	C→T	T261I (ACC→ATC)	flgJ →	putative peptidoglycan hydrolase FlgJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,138,152	0	C	T	100.0%	61.9 / NA	21	T261I (ACC→ATC)	flgJ	putative peptidoglycan hydrolase FlgJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/11);  total (10/11)

ACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATT  >  NC_000913/1137907‑1138392                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 actggactcggattGGGGACAACGGCAAGTCCGCCGCGAAAACGGAGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTAACTGAAATCACCGCGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGGCTGCAGCTCGTATCTGGAAGCCTTGTCGGAGTACGTTGAGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGGCGAtcgccg                                                                                                                                                                                                                                             <  1:264478/239‑1 (MQ=255)         cTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCg                                                                                                                                                                                                                                     >  1:210413/1‑251 (MQ=255)          tGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGACGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCgg                                                                                                                                                                                                                                    >  1:100885/1‑251 (MQ=255)                gggtcAACGCCAACTCCCCCGGGAAAACGGCGAGCCGAGCTATAACCTGTTTGGAGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCGCGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAgg                                                                                                                                                                                                                              <  2:5217/247‑1 (MQ=255)                                                agccgagcTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGcc                                                                                                                                                                                              <  1:58991/251‑1 (MQ=255)                                                            aCCTGTTTGGTGGCAAAGCCTCCGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGGTAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTTGGCTGTTAACCCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCAc                                                                                                                                                                                  <  1:259153/251‑1 (MQ=255)                                                                                     cAACGGGAAAGGGCCAGTTACTGAAATCCCCACGCCTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGAGAAGTTTCGCGTCTACAGCCCGTATCTGGAGGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCTCCAACATGa                                                                                                                                                         <  2:146304/251‑1 (MQ=255)                                                                                                      ttACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCg                                                                                                                                        >  1:22280/1‑251 (MQ=255)                                                                                                        aCTGAAATCACCACGACTGAATAAGAAAACGGCGCAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACATCTCGTATCTGGAAGCCTTGTCGGATTCCGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGCCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGAt                                                                                                                                      <  2:72435/251‑1 (MQ=255)                                                                                                           gAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTGAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAg                                                                                                                                   <  1:46314/251‑1 (MQ=255)                                                                                                           aaaaTCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTGCGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAg                                                                                                                                   <  2:200693/250‑1 (MQ=255)                                                                                                                caccacGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTAAGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACAAACATGATTCAGCAGATGAAATCGATAAGCGAc                                                                                                                               >  1:165751/1‑250 (MQ=255)                                                                                                                  ccacGACTGAATATGAAACCGGCGAAGCGAAGAAAGTAAAAGCGAAGTTCCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAg                                                                                                                            <  2:210413/251‑1 (MQ=255)                                                                                                                                            gcgaagAAAGTAAAAGCGAAGTTGCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTTCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGAGCCTCACTATGCCCGCAAATGCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACAt                                                                                                  <  1:178738/251‑1 (MQ=255)                                                                                                                                                                  ttCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACt                                                                            >  2:170176/1‑251 (MQ=255)                                                                                                                                                                                 cGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGAGTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCGACAGTATGAACAGTGATAATCTGTTCTGAATAACTCAAGTCCTGCggg                                                               <  2:182496/249‑1 (MQ=255)                                                                                                                                                                                    agcgggAAGCCTTGTCGGATTACGTTGGGCGTTTAACGCTTAACCCTCGCTACGCCGCCGGGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAATTCCGGCGGGTCGCt                                                          <  2:165751/247‑1 (MQ=255)                                                                                                                                                                                       tGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGcc                                                       >  2:222615/1‑251 (MQ=255)                                                                                                                                                                                              cTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGataat                                                 >  1:205825/1‑250 (MQ=255)                                                                                                                                                                                                   cGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGAAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCAGGCGGGTCGCTGCCGATAATACTCTg                                           >  1:237633/1‑251 (MQ=255)                                                                                                                                                                                                      aTTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGATCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTa                                         >  1:165724/1‑250 (MQ=255)                                                                                                                                                                                                                                            gccgTGACGATCGCCGCGAGTGCGGAACAGGTGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGAtt  >  1:219029/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 ACTGGAATCTGGTTGGGGGCAACGGCAAATCCGCCGCGAAAACGGCGAGCCGAGCTATAACCTGTTTGGTGTCAAAGCCTCTGGCAACTGGAAAGGGCCAGTTACTGAAATCACCACGACTGAATATGAAAACGGCGAAGCGAAGAAAGTAAAAGCGAAGTTTCGCGTCTACAGCTCGTATCTGGAAGCCTTGTCGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAACCTACAGTATGAACATTGATAATCTGTTCTGAATAACTCAAGTCCGGCGGGTCGCTGCCGATAATACTCTGTAATTGAAGGCTTATAAGGAACCTCCATGTCCAGCTTGATT  >  NC_000913/1137907‑1138392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑