Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,134,466	C→T	A222V (GCC→GTC)	flgF →	flagellar basal‑body rod protein FlgF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,134,466	0	C	T	91.3%	58.0 / NA	23	A222V (GCC→GTC)	flgF	flagellar basal‑body rod protein FlgF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (7/14);  minor base G (0/2);  total (7/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCA  >  NC_000913/1134227‑1134698                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         aaTCTCGGCGCTCAATCCGGGCTATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCTTTTAAGCGCAGAATCCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACTTCAATGCTGTTGCGGCAATGAGCGACAAGATTGGCAGCGCgcggc                                                                                                                                                                                                                               <  2:263304/251‑1 (MQ=255)         cgcTCAATCCGGTCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCTACATGATTGTCAGCGCGCGGCGTTTTGaa                                                                                                                                                                                                                       <  2:258693/251‑1 (MQ=255)                       gATCCGGCAAATGCGGTTGCGCCAGGAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCTGATGAAGGt                                                                                                                                                                                                         <  2:163620/251‑1 (MQ=255)                          ccGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGAt                                                                                                                                                                                                      >  1:151682/1‑251 (MQ=255)                          ccGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGGGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGGTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGa                                                                                                                                                                                                       <  1:31172/250‑1 (MQ=255)                                   aCGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATACCGTTGCGGCAATGAGCGACATGATTGTAAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGt                                                                                                                                                                                             >  1:10012/1‑251 (MQ=255)                                    ggggtGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACTACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGGTCTGGAATGCAGTAACGTCAATGCCGTTGCGTCAATGAGCGACATGATTGTCAGCGCGGGTCGGTTTGAAATGGAGATGAAGGTGAGCAGCAGCGTc                                                                                                                                                                                            <  2:44903/247‑1 (MQ=255)                                    cGGTTGCGCCAGTAGGGCGGCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTc                                                                                                                                                                                            <  2:119833/251‑1 (MQ=255)                                            ccAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGAGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATa                                                                                                                                                                                     >  1:121304/1‑250 (MQ=255)                                                 ggggCGTCTTAAACTGGTGAAAGCCGCGGGCAGCGAAGTGCAGCGCGGTGACGACGGCAATTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGGCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGGCGATGATAACGCAg                                                                                                                                                                               <  2:108059/250‑1 (MQ=255)                                                                 gTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAAct                                                                                                                                                               >  1:58954/1‑251 (MQ=255)                                                                        ccACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCg                                                                                                                                                        >  1:40231/1‑251 (MQ=255)                                                                           cGGGCTGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAATCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTTCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGGGCCAACCAACTGCTGTCGATg                                                                                                                                                     <  2:22027/251‑1 (MQ=255)                                                                            gggCAGCGAAGTGCAGCGCGGTGTCCACGGCACTTTTCGTTAAAGCGTCGAAACCCGGGCCAAGCGTGGGCGGGTAATGCAGTGAGAGCCAACCTTGCGTGGGATGTCGGGGGTTCTGGAAGGCAGTACCGTCAATGCCGTTGCGGCAATGAGCGACATGAGGGTCAGCGCGCGGAGTTGTGAAATGCAGATGATGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCTAACCAACTGCTGTCgctca                                                                                                                                                    <  2:220815/251‑5 (MQ=255)                                                                            gggCAGCGAAGTGCAGCGCGGTGACGTCGGCATGTTTCGTTTAAGCGCAGAAACCCAGGCCACGCTTGTGCCGGTACTGCAGGCAGATCCATCCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACGGCTGTCGATGa                                                                                                                                                    <  2:184335/251‑1 (MQ=255)                                                                               cAGCGAAGGGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGtt                                                                                                                                                 <  2:253926/251‑1 (MQ=255)                                                                                        gCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGaa                                                                                                                                          >  1:30497/1‑249 (MQ=255)                                                                                                gTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATg                                                                                                                                <  1:84096/251‑1 (MQ=255)                                                                                                        ggCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGtat                                                                                                                        <  1:64095/251‑1 (MQ=255)                                                                                                             ttttCGTTTAAGCGCAGAAACCCAGGCCACGTGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGTGGTTCTGGAATGCAGTAACTTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATTCATATGAAGGTGATTAGCAGTTTCGATGATAACGCAGGCCGTGCCAACTAACTGCTGGCGGTGAGTTAATTGAAAGGATACATGCCAAGTATAAGt                                                                                                                    <  1:257318/250‑1 (MQ=255)                                                                                                                                  ccAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGGCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTGGCCCGATGCGCAAGGTTATCg                                                                                              <  2:247497/251‑1 (MQ=255)                                                                                                                                                                                                                            ggCAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATg    >  1:226339/1‑251 (MQ=255)                                                                                                                                                                                                                              cAATGAGCGACATGATTGTCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGAGTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCGAGAAGACATATGCa  <  2:102074/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         AATCTCGGCGCTCAATCCGGGCGATCCGGCAAATACGGTTGCGCCAGTAGGGCGTCTTAAACTGGTGAAAGCCACGGGCAGCGAAGTGCAGCGCGGTGACGACGGCATTTTTCGTTTAAGCGCAGAAACCCAGGCCACGCGTGGGCCGGTACTGCAGGCAGATCCAACCTTGCGTGTGATGTCGGGGGTTCTGGAAGGCAGTAACGTCAATGCCGTTGCGGCAATGAGCGACATGATTGCCAGCGCGCGGCGTTTTGAAATGCAGATGAAGGTGATCAGCAGCGTCGATGATAACGCAGGCCGTGCCAACCAACTGCTGTCGATGAGTTAATTGAAAGGATACATGACAAGTATAAGTTGCCCGATGCGCAAGTTTATCGGGTCTATGGGGGCAATCGCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGAAGACATATGCA  >  NC_000913/1134227‑1134698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑