Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,133,273	G→A	G234S (GGC→AGC)	flgE →	flagellar hook protein FlgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,133,273	0	G	A	100.0%	52.3 / NA	20	G234S (GGC→AGC)	flgE	flagellar hook protein FlgE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/9);  total (11/9)

CATCGATGCAGATCAACCTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGACGGTACGGTTGTCGGCAACTATTCCAACGAACAAACCCAACTGCTGGGG  >  NC_000913/1133028‑1133509                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             cATCGATGCAGATCCCCCCGAATTCCAGTGATCCGATTCCTACTGTAACGCCATTCAGCGCCACCAATGCGGATAGCTATAACAAACCAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCCGTGATCCAAACAGCATTGCGAAGACAGCGCCAACACTGGAATTTACTGCTAATAGcac                                                                                                                                                                                                                                          >  2:111559/1‑250 (MQ=255)                      aTTCCAGTGATCGGCTTCCTACTGTTACGCCATTCAGCCCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGAGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGACTTGCGAAGACAGCGACAACACTGGAATATCATGCAAATAGAACATTAACGGGTGGTGCGATGGCg                                                                                                                                                                                                                    >  2:52198/1‑251 (MQ=255)                                                                   aTGCGAATAGCTATAACAAAACAGGTTCGGAGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTGAAAACCGGGGATAATAACTGGCCGGTCTACACCCAGGATAGCAGTGATCCAAACCGCATTGCGAAGGCAGCGACAACACTGGAATTTAATGCTAATAGCACATTAGTGGATGGTGCGATGGCGCATAATATCGCAACCGGCGCAATTAAAGGTGCATAACCCGCCACg                                                                                                                                                                       >  1:51543/1‑251 (MQ=255)                                                                              tataACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATAGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTg                                                                                                                                                              >  2:69007/1‑249 (MQ=255)                                                                                                         ttCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACCCCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATAGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATa                                                                                                                                  <  1:149669/250‑1 (MQ=255)                                                                                                          ccgccAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTGAAGCCTGGGGATAATAACTGGCAGGTCTCCACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATAGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGACCCCGCCTCGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATAcc                                                                                                                                <  1:111559/247‑1 (MQ=255)                                                                                                           cGACAGTAAGGTTAATGCTCATGACATGGGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTCCACCAAGGATAGCAGTGATACAACCAGCATTGCGAAGACAGCGACAACACGGGAATTTAATGTTAATAGCACATTAGTGGAGGGTGCGATGCCGAATAATATCGGAACCGGCGCAATTAACGTTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATAcc                                                                                                                                <  2:10294/250‑1 (MQ=255)                                                                                                                      gTAATGCTCATGACATGAGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATAGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAat                                                                                                                    >  1:206664/1‑251 (MQ=255)                                                                                                                            cTCATGACATGAGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATAGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATtgtg                                                                                                              >  1:113125/1‑251 (MQ=255)                                                                                                                              catgacatgaGCGTCTACTTGGTGAAGACCGGGGATAATAACTGGCAGGTCTACCCCCAGGATAGCAGTGATCAAAACAGCATTGCGAAGACAGCGAAAACACTGGAATTTAAGACTAATAGCACATTAGGGGATGTTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATtgtg                                                                                                              <  2:79819/249‑1 (MQ=255)                                                                                                                                     tgaGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATAGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAaccacc                                                                                                     >  2:17987/1‑251 (MQ=255)                                                                                                                                              actgtgggaaaaCCGGGGATAATAACTGGCAGGTCTACACCCGTGATAGAAGTGATCAAAACAGCATGGCGAAGGAACCGAAAACATTAGAAATTAATGCAAATAGAATATTAGTGGATGGTTCGATGGCGAATAATAGCGAAACCGTTGCAATTAACGGACCAGATACCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGTCAACCACCCAGAAc                                                                                               <  2:14558/237‑1 (MQ=255)                                                                                                                                                       aGACCGGGGATACTAACTGGCAGGTCTACACCCAGGCTAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATAGCACATTAGTGGATGGAGCGCTGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGACTGCGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGACAGGCTACAAACCg                                                                                   >  2:126914/1‑251 (MQ=255)                                                                                                                                                          ccGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATAGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGc                                                                                >  1:251236/1‑251 (MQ=255)                                                                                                                                                                                             aGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATAGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGacggtac                                             <  1:168346/251‑1 (MQ=255)                                                                                                                                                                                             aGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATAGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGacggta                                              <  1:163497/250‑1 (MQ=255)                                                                                                                                                                                                  tGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATAGCACATTAGTGGATGGTGCGATGGAGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCgg                                                                                  >  2:181428/1‑209 (MQ=255)                                                                                                                                                                                                  tGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATAGCACATTAGGGGATGGTGCGATGGCGAATAATATCGCACCCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCgg                                                                                  <  1:181428/209‑1 (MQ=255)                                                                                                                                                                                                                        aCAGCGACAACACTGGAATTTAATGCTAATAGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGACGGTACGGTTGTCGGCAACTATTCCAACGAACa                  <  1:17972/251‑1 (MQ=255)                                                                                                                                                                                                                                        aaTTTAATGCTAATAGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGACGGTACGGTTGTCGGCAACTATTCCAACGAACAAACCCAACTGCTgggg  >  1:78352/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                             CATCGATGCAGATCAACCTGAATTCCAGTGATCCGCTTCCTACTGTTACGCCATTCAGCGCCAGCAATGCGGATAGCTATAACAAAAAAGGTTCGGTGACTGTTTTCGACAGTCAGGGTAATGCTCATGACATGAGCGTCTACTTTGTGAAGACCGGGGATAATAACTGGCAGGTCTACACCCAGGATAGCAGTGATCCAAACAGCATTGCGAAGACAGCGACAACACTGGAATTTAATGCTAATGGCACATTAGTGGATGGTGCGATGGCGAATAATATCGCAACCGGCGCAATTAACGGTGCAGAACCCGCCACGTTTAGTCTGAGCTTCCTCAACTCCATGCAGCAAAATACCGGCGCTAACAATATTGTGGCAACCACCCAGAACGGCTACAAACCGGGCGATCTGGTGAGTTATCAAATCAATGATGACGGTACGGTTGTCGGCAACTATTCCAACGAACAAACCCAACTGCTGGGG  >  NC_000913/1133028‑1133509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑