| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,130,803 | C→T | A21T (GCG→ACG) | flgA ← | flagellar basal body P‑ring formation protein FlgA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,130,803 | 0 | C | T | 95.5% | 57.5 / NA | 22 | A21T (GCG→ACG) | flgA | flagellar basal body P‑ring formation protein FlgA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (14/7); minor base G (0/1); total (14/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 | |||||||||||
GCATCGCGGCAACCACATAATTTCCTGTGGCCTGTACATTAACCTGTAAATATCGTTTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACT‑GAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGAATTTTGATACCTGCGGAGGAGATAT > NC_000913/1130557‑1131019 | gCATCGCGGCAACCACATAATTTCCTGTGGCCTGTACATTAACCTGTAAATATCGTTTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGAGATTGCTCGTCGTa < 1:70334/251‑1 (MQ=255) aaTTTCCTGTGGCCTGTACATTAACCTGTAAATATCGTTTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTTCTCATCGAAAACAATGGCTGCTCGCATAGCGGGAGGAGATTGGGGGCTGTACGAATAACAACAAGAACCTCATCCCCTACCCCCGCGAGCTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGAGGTTGCTCGTCGGTCTTATCGGACT‑GAACAgg > 1:48516/1‑250 (MQ=255) ccTGTACATTAACCTGTAAATATCGTTTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTTAGATTGCTCGTCGTACTTAACGGACT‑GAACAGTATCGCGATGATc < 1:46375/251‑1 (MQ=255) gTACATTAACCTGTAAATATCGTTTGGCGTTACCGCAGCGTGCCAACACATTCACATTGCACCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAAACTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAAGTGCGATGTGAGATTGCTCGTCGTACTTAACGGACT‑GAACAGTATCGCGATGATCGcc < 1:192409/251‑1 (MQ=255) aaCCTGTAAATATCGTTTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGACTTATTGCTCATCGAAAGCAATAGCTGCTCGCATGGGGGGAGTAGATTTGGCGCTGTAAGAATAAAAACACGGAACTCATCAATTACCCCCGCGAGTTGGGTGCTAAAAAAATTTGTG‑AAATGGGGTGTGGGATTGTTCGTCGTACTTTATGGGCTAGAAC‑GTATCGCGGTGATCGACACGCtcc > 2:28212/1‑249 (MQ=255) aaaTATCGTTTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGATGCTCGCATGGCGGTAGAAGATTGGGGGCTGTACGAAAAGAAAAACGAACCTCATCACTTAACCCCGCGGGTTTGGCGCTTAAAAA‑ATTGTGCAATTGCGCTGTGAGAATGCTCGTCGTACATAAAGGACT‑GAACAGGATCGCGATGATCGCCACGCCACGGTttat > 1:63080/1‑251 (MQ=255) atcttttGTCGTTACCGCAGCGTGCCAACACATTCACATGGCCCCACAGGCGGGAATTATTGCGCAGCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATGGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACT‑GAACAGTAGCGCGATGATCGCCACGCTACGTTTtattat < 2:118742/246‑1 (MQ=255) gTCGTTACCGCAGCGTGCCAACACATTCACATTGCCTCACAGGCGGGAATTAGTGCTCATCGAAAGCAATGGCTGCTCGCATGGCTGTAGTAGATTGGGCGCTGTTCGAATAGAAACACGAACCTCATCACTTTCCCCCGCGAGTTGGGCTCGAAAAAA‑GTGGTGCAATTGCGAGGTGAGATTGCTCGGCGTGCGTAACGGACT‑TAACAGTAGCGCTATGATCGCCACGGTACGTTTTATTATCAGCAtt < 2:260483/250‑1 (MQ=255) gTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACCAACCTCATCACTTAACCCCCCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGGGAGATTGCTCGTCGTACTTAAAGGACT‑GAACAGTATCGCGATGATCGCCCCGCTCCGTTTTTTTATCAGCAttt > 1:32743/1‑251 (MQ=255) gAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGAACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACT‑GAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCCTTTTCGCCCCCAGCCATTTCTACAACGTGACTTGTACCTGTCCGCAATGACCATCAACGGCAt > 2:194508/1‑250 (MQ=255) aaGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACT‑GAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATaa > 1:79982/1‑250 (MQ=255) aTGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACT‑GAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCg < 2:32743/251‑1 (MQ=255) agtagATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACT‑GAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTcc > 1:72623/1‑251 (MQ=255) gattgagagcTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGAGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACT‑GAAAAGTATCGCGATGATCGCCACGTTACGTTTTATTATCAGAATTTTCTCCCCCAGCCATTTCTACAACGTGAATTGCACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTccgc > 2:76004/9‑249 (MQ=255) gcTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAACTGCGCTGCGAGATCGCTCGTCGTACTTAACGGACT‑GAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGACCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCACCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAAcg > 1:255299/1‑249 (MQ=255) aCGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGCCT‑GAACAGTATCGCGATGATCGCCACACTAAGTTTTATTATCAGCATTTTCCCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAACTAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGt > 1:194832/1‑251 (MQ=255) cGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACT‑GAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGt > 1:37639/1‑250 (MQ=255) aGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACT‑GAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAgg < 1:265127/250‑1 (MQ=255) cTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGCGATTGCTCGTCGTACTTAACGGACT‑GAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGAACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGa > 1:204492/1‑250 (MQ=255) ccccGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTAACGGACT‑GAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGAAGTTTg > 2:209999/1‑251 (MQ=255) cgcTAAAAAA‑TGTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTACCGGACT‑GAACAGTGTCGCGATGATCGCCACGCTCCTTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGGGAATTGTTCCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGAATTTTGATACCTGCggagga < 2:63080/251‑1 (MQ=255) aaaaa‑GTTGTGCAATTGCGATGTGAGATTGCTCGTCGTACTTACCGGACT‑GAACAGTCTCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGCTAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGAATTTTGATACCTGCGGAGGAGatat > 2:132345/1‑251 (MQ=255) | GCATCGCGGCAACCACATAATTTCCTGTGGCCTGTACATTAACCTGTAAATATCGTTTGTCGTTACCGCAGCGTGCCAACACATTCACATTGCCCCACAGGCGGGAATTATTGCTCATCGAAAGCAATGGCTGCTCGCATGGCGGTAGTAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAA‑GTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACT‑GAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAAGGCATTTAAGCTGATGGCAGAATTTTGATACCTGCGGAGGAGATAT > NC_000913/1130557‑1131019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |