Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,130,210	G→A	N218N (AAC→AAT)	flgA ←	flagellar basal body P‑ring formation protein FlgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,130,210	0	G	A	100.0%	54.5 / NA	18	N218N (AAC→AAT)	flgA	flagellar basal body P‑ring formation protein FlgA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/10);  total (8/10)

GCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGAT  >  NC_000913/1129986‑1130429                                                                                                                                                                                                                                 |                                                                                                                                                                                                                            gcttgcGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATGCCCATCTGCATc                                                                                                                                                                                                   <  2:129390/251‑1 (MQ=255) gcgtgcGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTGCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTGATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTGATAAGAATATTCCCATCTGCATc                                                                                                                                                                                                   <  2:230218/248‑1 (MQ=255)                                                     ccgccCGGATGGTCGTTACCGGCGCGTCCGAGGTTTCGCGCGGTTGACCGGTGCTTACAGGCTTCAGAGGCGAAGGGCGCTCAATACTCATGGTTTATTCCTCATTGCGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGAAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCACCAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTcagt                                                                                                                                               >  2:170116/1‑248 (MQ=255)                                                              tGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAAcg                                                                                                                                                                                            >  1:105269/1‑197 (MQ=255)                                                              tGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAAcg                                                                                                                                                                                            <  2:105269/197‑1 (MQ=255)                                                                              ggcAGTGGGTTCGGGCGGGTGAACGGTGATGACAGGCTGCAGAGGGGAAGTGCGAGCGATGCGCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGGTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCt                                                                                                                      <  2:232030/249‑1 (MQ=255)                                                                                 aGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGAc                                                                                                                   <  1:47536/251‑1 (MQ=255)                                                                                           cgcgGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGtt                                                                                                         >  1:17504/1‑251 (MQ=255)                                                                                                                               gTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGAc                                                                     <  1:97949/251‑1 (MQ=255)                                                                                                                                                    tATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCg                                                >  1:211024/1‑251 (MQ=255)                                                                                                                                                    tATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTAGCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTAccc                                                 <  1:250635/250‑1 (MQ=255)                                                                                                                                                            aTTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTTTGCGACGGCTTCATTGTTCAGCGCCTGACCTTCTGc                                                                                                            <  2:14903/183‑1 (MQ=255)                                                                                                                                                            aTTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTg                                                                                   <  1:50182/208‑1 (MQ=255)                                                                                                                                                            aTTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTg                                                                                   >  2:50182/1‑208 (MQ=255)                                                                                                                                                            aTTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCACATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGc                                                                                                            >  1:14903/1‑183 (MQ=255)                                                                                                                                                                                  gtTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGtt                                                                                                                              >  1:97175/1‑143 (MQ=255)                                                                                                                                                                                  gtTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGtt                                                                                                                              <  2:97175/143‑1 (MQ=255)                                                                                                                                                                                                   aaGGGACGGGTAATCTTTAACAGCTTACAGATTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGAt  >  1:93621/1‑250 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                            GCTTGCGCGTCGCTTAACGTCACACTGGTGCTGGTGGAGGCGGTTGTTTTTGCCGCCCGGCTGTTCGTTACCGGCGCGTCAGTGGTTTCGCGCGGTTGAACGGTGCTTACAGGCTTCAGAGGCGAAGTGCGATCAATACTCATGGTTTATTCCTCATTGAGGGCGCTTTTATCATGTGTTGCTTATTTATCGGCAAGGGACGGGTAATCTTTAACAGCTTACAGGTTTATAAGAATATTCCCATCTGCATCAACAACGCCGCTGACTACCTGTCCCGATACCATGCGCACCCGCGCATTCTGTGCGACGGCTGCATTGTTCAGCGCCTGACCTTCTGCGTTGGCGCTAAACCCATCACCGCTGGCGATCACATTGACGCGTTGTCCCGCTTTTACCCGCCATGCCTGGCGAAACTGGGTTAACTGGATAGGTTGATCGGGTGAT  >  NC_000913/1129986‑1130429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑