Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,676	C→T	F780F (TTC→TTT)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,676	0	C	T	100.0%	57.8 / NA	21	F780F (TTC→TTT)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/7);  total (14/7)

CTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTACTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCTGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGG  >  NC_000913/2444‑2918                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    cTGTGCTGCCCGCAGAGTTTAACGCTGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGAGGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATc                                                                                                                                                                                                                                     >  1:249246/1‑249 (MQ=255)                       cgctgAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAGCTCGACGGTCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGGGGATGGTAATGATCAGCTGTTCAAAGTGAAAAATGGCGAAAACGACCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGcgcg                                                                                                                                                                                                             >  2:82174/5‑251 (MQ=255)                         cggAGGGTGATGTTGCCGCTTTTATGGGGAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAAGGGCGAAAACGCCCTGGCCTTTTAGAGCCACTATTATCAGCCGCGGCCGTTGGTGCTGCGCgg                                                                                                                                                                                                            <  2:215709/248‑1 (MQ=255)                                                     gAATCTGTCACAGCTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAACCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTg                                                                                                                                                                               >  1:3795/1‑251 (MQ=255)                                                                                      gcgcgTGGCGAAGGGCCGTGATGAAGGAAAAGGTTTGCGCTATGTTGGCAATATTGATGAAGAGGGTTCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCctct                                                                                                                                              <  2:160886/251‑1 (MQ=255)                                                                                         cgTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCAt                                                                                                                                           <  1:263224/251‑1 (MQ=255)                                                                                                aaGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGtt                                                                                                                                    <  2:3795/251‑1 (MQ=255)                                                                                                        tgatgaAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCt                                                                                                                            >  2:7167/1‑251 (MQ=255)                                                                                                               ggataaGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGTGACTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAACTGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGACCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATgg                                                                                                                     >  2:61394/5‑251 (MQ=255)                                                                                                                              tATGTTGGCAATATTGATGAAGATGGTGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGCAGTTAGGAGTCTGACATGGTTAAAGTTTATGccc                                                                                                      >  1:17262/1‑251 (MQ=255)                                                                                                                               aTGTTGGCAATATTGATGTAGATGGAGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTATCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTCTGcccc                                                                                                     >  2:257595/1‑251 (MQ=255)                                                                                                                                                gaAGATGGAGCCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAACATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTCAAGTTTATGCCCCGGCTTCCAGTGCCAat                                                                                     >  2:129307/1‑250 (MQ=255)                                                                                                                                                  aGATGGTGCCTGCCGCGTGAAGATTGCCGCAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTCTCAGCCGCTGCCGTTGCTGCTGCGAGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATg                                                                                  >  1:244478/1‑251 (MQ=255)                                                                                                                                                                            ccGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGCGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGGGCCAAAATGAGCGTCGGGTTTGATGTGCTCgggcc                                                        >  1:1421/1‑249 (MQ=255)                                                                                                                                                                                aGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGgcggcg                                                    <  1:257595/251‑1 (MQ=255)                                                                                                                                                                                          aaTGAGCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCGGGCCGTTTATAGCCACTATTATCAGCCGCTGCCGTTAGTGCTGCGCTGatat                                                                                                                                                                                                        <  2:138839/93‑1 (MQ=255)                                                                                                                                                                                              aTCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGATAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTgg                                     >  2:2401/1‑250 (MQ=255)                                                                                                                                                                                                                  aaTGGCGAAAACGCCCTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGGTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGAATTGCTCGgagttg                 >  2:32890/1‑248 (MQ=255)                                                                                                                                                                                                                       cGAAAACGCCCTGTCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGGGCGGGCAATGACGTTACAGCTGCCTGTGTCTTTGCCGATCTGCTACGTACCCTCGCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTGCAGTGCCAATATGAGCGTCGGGTTTGATGGGCTCGTGGCGGCGGT‑GACACCTGTTGATGGTGCATTGTTCGGTGATGTAGTc            <  2:176925/251‑1 (MQ=255)                                                                                                                                                                                                                        gAAAACGCCCTGGCCTTTTATAGCCAATATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCACTATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGTTGA‑ATCTGTTGGTGGTGCATTgtatggagttggaagc            >  2:211390/1‑235 (MQ=255)                                                                                                                                                                                                                                 cTGGCCTTTTATAGCCACTATTATCAGCCGCTGCCGTTGGTGCTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCCGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAgg  >  1:1165/1‑251 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    CTGTGCTGCCCGCAGAGTTTAACGCCGAGGGTGATGTTGCCGCTTTTATGGCGAATCTGTCACAACTCGACGATCTCTTTGCCGCGCGCGTGGCGAAGGCCCGTGATGAAGGAAAAGTTTTGCGCTATGTTGGCAATATTGATGAAGATGGCGTCTGCCGCGTGAAGATTGCCGAAGTGGATGGTAATGATCCGCTGTTCAAAGTGAAAAATGGCGAAAACGCCCTGGCCTTCTATAGCCACTATTATCAGCCGCTGCCGTTGGTACTGCGCGGATATGGTGCGGGCAATGACGTTACAGCTGCCGGTGTCTTTGCTGATCTGCTACGTACCCTCTCATGGAAGTTAGGAGTCTGACATGGTTAAAGTTTATGCCCCGGCTTCCAGTGCCAATATGAGCGTCGGGTTTGATGTGCTCGGGGCGGCGGT‑GACACCTGTTGATGGTGCATTGCTCGGAGATGTAGTCACGGTTGAGG  >  NC_000913/2444‑2918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑